Chien-Hua Huang
National Pingtung University of Science and Technology, Taiwan
Title: Study on the protective effects of tocotrienols in SH-SY5Y cells
Biography
Biography: Chien-Hua Huang
Abstract
Tocotrienols and tocopherols, naturally occurring isoforms of vitamin E, are found abundantly in food such as palm oil, rice bran oil, corn, oats, barley, rye and wheat. Tocotrienols have four different isomers: α-, β-, γ- and δ-forms. The present study was designed to evaluate the protective effect of tocotrienols against methylglyoxal (MG)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. In order to assess the protective effect of tocotrienols, α-tocotrienol (α-T3), γ-tocotrienol (γ-T3) and δ-tocotrienol (δ-T3) were administered to SH-SY5Y cells for 2 hours prior to the induction of cytotoxicity by MG, and the cell viabilities of SH-SY5Y cells were analyzed by MTT assay. The cell viability of SH-SY5Y cells were reduced to 40% by 24 h treatment of 0.5 mM MG, and two hours pretreatment of α-T3, γ-T3 and δ-T3 increased the cell viability to 75%, 53% and 82%, respectively. It is shown that T3 has protective effect on the neuro-toxicity induction by MG. Among T3, δ-T3 has the best protective activity. The future work needs to be performed to elucidate the neuro-protective mechanism of T3.