Gulsum Gizem Topal
Hacettepe University, Turkey
Title: The Effect of Probiotic Bacteria on Aflatoxin M1 Detoxification in Phosphate Buffer
Biography
Biography: Gulsum Gizem Topal
Abstract
Statement of the Problem: Aflatoxin M1 (AFM1) is a major toxic and carcinogenic molecule in milk and milk products. Therefore, it poses a risk for public health. There are some studies that probiotics have a binding ability to AFM1, so that they can remove the AFM1 from yoghurt. The aim of this study is to evaluate the AFM1 binding ability of some probiotic bacteria in phosphate buffer saline.
Methodology & Theoretical Orientation: The PBS samples artificially contaminated with AFM1 at concentration 100 pg/ml were prepared with probiotics bacteria that including monoculture (L. plantarum, B. bifidum ATCC, B. animalis ATCC 27672) and binary culture (L. bulgaricus + S. thermophiles, B. bifidum ATCC + B. animalis ATCC 27672, L. plantarum+B. bifidum ATCC, L. plantarum+ B. animalis ATCC 27672). The samples were incubated at 37°C for 4 hours and stored for 1, 5 and 10 days. The toxin was measured by the ELISA.
Findings: The highest levels of AFM1 binding ability (63.6%) in PBS were detected yoghurt starter bacteria, while L. plantarum had the lowest levels of AFM1 binding ability (35.5%) in PBS. In addition, it was found that there was significant effect of storage on AFM1 binding ability in all groups except the one including B. animalis (p<0.05).
Conclusion & Significance: Results demonstrate that AFM1 detoxification by probiotic bacteria has a potential application to reduce toxin concentrations in yoghurt. Moreover, probiotic strains can react with itself as synergic or antagonist.