Track 1: Nutrition and Basic Science
Universidad Catolica San Antonio de Murcia (UCAM), Spain
Title: Virtual screening for the discovery of novel bioactive compounds: Application to blood anticoagulants and nutraceuticals
Horacio Pérez-Sánchez graduated in Theoretical and Computational Chemistry from the University of Murcia (Spain) in 1999, where he also obtained his PhD degree in 2006, which consisted in the development and implementation of computational methodologies for the study of macromolecular systems. He performed research in several European Institutions such as the Institute of Structural Biology (CEA, Grenoble, France), and at the Karlsruhe Institute of Technology (Karlsruhe, Germany). Currently he is full-time Researcher and Principal Investigator of the "Bioinformatics and High Performance Computing" research group at the Catholic University of Murcia (Spain). He has co-authored more than 50 international conference contributions and journal papers, book chapters and European patents. He has served as a reviewer for several conferences/journals/book editorials. His primary research interests are in the areas of bioinformatics, mainly drug discovery, and its exploitation in parallel architectures. He has participated in several projects funded by the European Commission and the Spanish and German Ministries of Science.
The discovery of novel anticoagulants without side effects is one of the main problems of haemostasis. Ever since the discovery of the anticoagulant properties of hirudin from the leech saliva, the increasing relevance of thromboembolic diseases has encouraged a continuous search for new compounds with anticoagulant activity, which have led to the development of the new commercially available anticoagulants. One of the targets for prophylaxis and treatment of thromboembolic diseases is the plasma anticoagulant antithrombin. This protein circulates in blood in a metastable conformation, in which the reactive centre loop is partially inserted and is only activated by heparin and heparan sulfate glycosaminoglycans on the injured subendothelium. Accordingly sulfated polysaccharide heparin chains with different size, from unfractionated to the essential pentasaccharide, have been used with more or less success in anticoagulant therapy and thromboprophilaxis. In the last decades new molecules able to bind antithrombin have been identified. The strategies used in this search have been based mainly on the synthesis or chemical modification of existing drugs, or in the application of natural compounds with similar properties to those currently used. Examples for such compounds are lignins and flavonoids, highly sulfated small organic ligands that seem to have similar properties to heparins. Therefore, the discovery of novel or improved drugs for given diseases or special groups of patients, is a very slow and expensive process. Nevertheless, recent results obtained by the speaker demonstrate the discovery using Virtual Screening (VS) of a novel molecular scaffold, different to the previous ones based on heparin. Using this alternate approach, a large database of millions of chemical compounds is screened in-silico and affinity-ranking is used to identify some at least weakly-binding molecules for further refinement. Aided by ever-increasing computational power, VS is an appealing and cost-effective approach to tap into the wealth of available structural information. Consequently, novel and enhanced VS methodologies, conveniently exploited in innovative drug discovery strategies can lead to significant and quantifiable developments. In addition, examples of the application of this methodology to the characterization and discovery of bioactive compounds present in food will be given.
Director of the Research Centre on Health & Nutrition CEIDSS
Title: Socioeconomic factors on school aged children´s overweight and obesity in the context of a community based program - MUN-SI, Portugal: A contribution for the EU project OPEN
Ana Rito is the Director of the Research Centre on Health & Nutrition CEIDSS. She graduated in Nutritional Sciences (Portugal), obtained a Master Degree in Medical Sciences (UK) and her PhD in Public Health (Brazil). From 2008 she undertook 2 Post-Doc at the National Institute of Health Doutor Ricardo Jorge – Portugal, one in the EU project EConDA- Economics of Chronic Diseases" led by the UK Health Forum. Former Vice President of Scientific Committee of the Platform Against Obesity (Ministry of Health) and the Head of the Bachelor Degree in Nutritional Sciences – University Atlântica, she is currently the Principal Investigator (PI) of Projects based on the development of creative answers to tackle childhood obesity of a municipality-level. She is the PI for Portugal, of the EU project “OPEN” and of the study COSI of the WHO/Europe and a member of its Executive Committee. She is also a member of European Networks and recent collaborations include the Copenhagen Business School and Metropolitan University College (DK).
Portugal is one of the countries within the WHO European Region with higher prevalence of childhood overweight (over 30%) and obesity but not all children are affected equally by the burden of obesity and poor health. Children in families that do not have adequate resources are more likely to be obese and face a greater burden of ill health than children who grow up in families that are better off. Based on the rationale that local governments exert an important and decisive role in counteracting childhood obesity, MUN-SI program was developed in Portugal. MUN-Si is an on-going community-based program at local level which aims to promote lifestyles changes in the long-term, and it is also a member of the EPODE International Network. Along with other 11 European programs, MUN-SI is a partner in the EU project “OPEN”, an innovative project to scale up efforts to prevent childhood obesity across Europe. In order to fully contribute to the design of local actions to promote active living and healthier dietary behaviours, and detailed children´s nutritional status assessment, evaluation and association of its multivariable factors including socio-economic was needed to support following interventions. The objective of this study was to address the association between socioeconomic indicators and children´s nutritional status (NS) of the two rounds of the MUN-SI NS surveys (2009 and 2011). It was performed an observational cross-sectional study of elementary school children from five cities. A total of 2726 children (round 1 (R1) =1126; round 2 (R2) =1600) aged between 6-12 years old were evaluated. The childhood overweight and obesity prevalence was assessed according to WHO criteria, adopted by the Ministry of Health of Portugal. Low socioeconomic status was defined according to three criteria: family income, parents occupation and education level. These socioeconomic characteristics were obtained by a self response questionnaire developed within the MUNSI program. In R1 (2009), the prevalence of overweight was 39.4% (n = 444), of which 15.8% (n = 178) were obese. In the second round (2011) similar prevalence were obtained (40.8% of overweight, in which 16.9% obese). In both rounds, 69% of the families had a low income (<1500 €). Mother's education level was mainly up to 12th grade (79%) and more than 50% of fathers had an education level up to 9th grade. Parents occupation were manly (>45%) unqualified or semi-qualified. In R1, a family income of 0-1500 € was associated with higher obesity prevalence (OR=2.37; IC95%: 1.11-5.02). In R2, no significant association between obesity prevalence and low family income (p=0.494) was observed. Families where the parents had a non-qualified or semi-qualified occupation had a higher probability (mother - OR≥3.4; father - OR≥2.7) to have children with obesity. Low education level of the parents was also proved to be associated with higher childhood obesity prevalence. This study observed that low socioeconomic status is an important risk factor to development of childhood obesity in Portuguese population. These results support that further intervention is needed on low socio-economic families.
Maria Jose Frutos Fernandez
Universidad Miguel Hernández de Elche, Spain
Title: Polyphenols processing stability and interactions with other compounds
María José Frutos-Fernández has completed her PhD in Biology in 1995 from University of Murcia and has 15 years of professional activity as researcher and professor in food quality and safety. She is the deputy director of the High Polytechnic School of Orihuela, and professional member of the Institute of Food Technologists, of the IFT Annual Meeting Scientific Program Advisory Panel and of R&D expert panels for international agencies. Her main field of research is functional foods. She has led European and national projects on bioactive ingredients. Her current research focuses on Probiotic vegetable foods and viability after digestion.
Polyphenols are compounds of the secondary metabolism that can be attributed a bioactivity mainly due to their properties as antioxidants or metal chelators. Numerous studies demonstrate their benefits in the prevention of several types of cancer and cardiovascular diseases, together with their anti-inflammatory and antimicrobial activity. These compounds are abundant in the diet because they are widely distributed in nature as part of fruits, legumes and vegetables.
There are numerous factors that can affect to the type and content of biologically relevant polyphenols, during crop, storage and processing. Vegetable processing involves different operations and physico-chemical treatments that can lead to changes in the composition, in particular of polyphenols. These compounds can also be added during functional food development and therefore different strategies are investigated to improve their stability. The way of being metabolized depends mainly upon their chemical form that determines their solubility, and this property can also be modified by processing or intended biotransformation. Polyphenols can also interact with other food compounds as proteins and lipids with consequences on their bioactivity. Polyphenol-protein interactions could have a marked effect on the bioavailability and function of polyphenols and could have some effects on physical properties or texture characteristics in foods. Anthocyanins are polyphenolic pigments that can be also affected by food composition, thermal treatment and other factors. Polyphenols can also interact with gut micro biota with implications on their digestive absorption, metabolism and bioactivity.
University of Barcelona, Spain
Title: Nutritional biomarkers after polyphenol-rich diets: From database development to clinical trials application
Mireia Urpi-Sarda has completed her Ph.D at the age of 30 years from University of Barcelona. She did predoctoral stages in the Institute National de la Recherche Agronomique, INRA (France) and postdoctoral studies from several stages in Aberystwyth University (United Kingdom), Perugia University (Italy) and Hospital Clinic of Barcelona (Spain). She is an associate scientist (Ramon y Cajal Program) in the Biomarkers and Nutritional & Food Metabolomics Research Group in the Nutrition and Food Science Department in the University of Barcelona. She has published more than 50 papers in reputed journals and more than 10 international book chapters.
The knowledge of in vivo polyphenol metabolites derived from the consumption of polyphenol rich foods, including those that could come from microbiota metabolism, would help and could be a key to understand these benefits. New screening strategies need to be developed to evaluate these metabolites as biomarkers of intake. The discovery of biomarkers of intake through metabolomics approaches is essential to associate dietary intake (considering their bioavailability) and diet-related risk factors for diseases in nutritional epidemiological studies. Phenol-Explorer 2.0 database has been created to integrate data on polyphenol metabolites with pharmacokinetic data in vivo studies in humans and experimental animals. This database is useful for planning experiments to elucidate the effects of polyphenols and health and to respond to current needs to the rapid identification of polyphenolic biomarkers in complex fingerprints obtained in metabolomic experiments. This database was applied to the metabolism of polyphenol-rich foods consumption to predict the wine polyphenol metabolic space. After developing a flow-chart search strategy, 97 metabolites were retrieved related with wine consumption corresponding to host and microbial metabolites of several wine polyphenols. The linking of metabolites among them generated a global pathway map of wine polyphenols metabolism which does not exist in current available resources or databases. The use of Phenol-Explorer 2.0 in helping in the identification of biomarkers of polyphenol-rich foods consumption will improve in the understanding of polyphenol health effects.
Universite de Bordeaux, France
Title: Comparative study between seeds of spine and spineless Opuntia ficus-indica
Nadia Chougui is a teacher at the University of Bejaia (Algeria) and she is preparing her doctoral thesis programmed for this month. She has published 3 papers and others, are under process. She works with other laboratories (Nancy, France), mainly, on Opuntia ficus-indica valorization.
Seeds of spine and spineless Opuntia ficus-indica varieties were investigated. This comparative study aimed to find out if the presence or the absence of spines could have an effect on the seeds composition. Morphological and physical characteristics of the fruits were first registered. Seeds of each variety were separated, dried and reduced to powder. This powder was subjected to oil and phenolic extractions. Determination and characterization of fatty acids and phenolic compounds were realized using, respectively, gas chromatography and liquid chromatography coupled to mass spectrometry (LC-MS (n)) and to nuclear magnetic resonance (LC-NMR) approaches. In addition, an evaluation of the antioxidant and the antiradical activities of the phenolic extracts were investigated. The statistical analysis of the obtained data revealed significant differences at p≤0.05. Indeed, higher oil rate was obtained in the spineless variety and GC analysis of the seed oils showed high percentages of total unsaturated fatty acids (TUFA), particularly linoleic acid up to 63%. The highest level of TUFA was also found in the spineless variety, contrarily to the total saturated fatty acids (TSFA) level. The phenolic profile of the seeds of both varieties displayed a high complexity, with more than 20 compounds detected at 330 nm. However, the phenolic compounds amounts were much higher in the spine variety as well as the antioxidant and the antiradical activities. The data indicated that seeds of both varieties contain various components that constitute a source for natural substances. The spineless variety presents a good source of oil while the spine one contains more antioxidants.
Marina M Belluci
University Estadual Paulista, Brazil
Title: The influence of magnesium deficiency on bone remodeling cells
Marina Montosa Belluci graduated at School of Dentistry at Araraquara – UNESP (2006). Master in Periodontics, School of Dentistry at Araraquara – UNESP (2006 - 2008) and Doctorate in Dentistry from the Universidade School of Dentistry at Araraquara – UNESP (2008 - 2012). Held part of her doctorate degree at Vrij Universiteit - Netherlands (2010-2011). Postdoctoral at School of Dentistry at Araraquara – UNESP (2012 - 2013).
It is still not clear how magnesium (Mg+2) deficiency affects bone remodeling system. Bone marrow cells from long bone of mice were seeded on plastic and on bone in medium containing different concentrations of Mg2+ (0.8 mM which is 100% of the normal value, 0.4, 0.08 and 0 mM). The effect was evaluated on osteoclasts precursors to its viability and proliferation rate after 3 and 6 days, as was mRNA expression of osteoclastogenesis-related genes and Mg2+-related genes. After 6 days of incubation, the number of tartrate resistant acid phosphatase-positive multinucleated cells (TRACP+-MNCs) was determined, and the TRACP activity of the medium was measured. Osteoclastic activity was assessed at 8 days by resorption pit analysis. Also it was evaluated, in vivo, Mg2+ deficiency on mandibular bone loss associated with ligature-induced periodontitis. For this study, ten rats were randomly divided into two groups: control - animals fed a standard diet; Mg- animals fed with 90% of Mg2+ deficiency diet. After 60 days on the diets, all animals received ligature, and were euthanized after 30 days. The lumbar vertebrae were collected for micro-CT evaluation. The mandibles were collected for immuhistochemistry analysis. The in vitro study showed that Mg2+ deficiency resulted in increased numbers of osteoclast-like cells. Increased osteoclastogenesis due to Mg2+ deficiency was reflected in higher expression of osteoclast-related genes. However, resorption per osteoclast as well as TRACP activity were lower in the absence of Mg2+. The in vivo study demonstrated a bone mass loss in Mg2+ deficiency rats. The immunohistochemistry showed an increased expression of INOs+ cells and decreased OCN+. In conclusion our data suggest that Mg2+ deficiency in vitro altered osteoclast numbers and activity. In vivo studies suggests that the inflammation stimulus during Mg2+ deficiency may play an important role not only on osteclasts but also on osteoblasts cells and oxidative stress.
Centro Universitário Metodista IPA, Brazil
Title: Grape juice: Its compounds and health benefits
Caroline Dani graduated from the University Center BIOMEDICINA Feevale (2004). She completed her Master’s in Biotechnology from the University of Caxias do Sul (2006) and Ph.D. in Biotechnology from the University of Caxias do Sul (2008). She is currently a full Professor at Methodist University IPA, working on undergraduate and Graduate Stricto sense, Biosciences and rehabilitation and Reablilitação and Inclusion programs. She is a Coordinator of the Graduate Program in Bioscience and Rehabilitation and defended her dissertation and thesis based on the benefits of grape juice. She has experience in the area of nutrition, with emphasis on Biochemistry of Nutrition, acting on the following topics: juice, organic, conventional, polyphenols, antioxidant, grape juice and oxidative stress, flavonoids, portal hypertension.
Experimental data have increasingly suggested that cellular oxidative damage has a relevant pathophysiological role in several types of human diseases, such as atherosclerosis and cancer. In order to minimize oxidative stress, our cells have developed a complex biochemical redox mechanism, consisting of both enzymatic and non-enzymatic components. Moreover, the diet, especially the consumption of fruits and vegetables, also has an important role in the maintenance of physiological redox equilibrium. These foods supply several antioxidants, including several polyphenolic compounds to the body. Grapes are rich in phenolic compounds, such as flavonoids (catechin, epicatechin, quercetin, anthocyanins, procyanidins), and resveratrol (3,5,4`-trihydroxy-stilbene), which are mainly found in red grape products. Nowadays, many studies showed the grape juice’´s health benefices. All findings suggest that grape juices induce significant antioxidant, neuroprotec, hepatoprotec, antiplaquetary, antitumoral, antimutagenic, antigenotoxic activities and also, increase the memory , and this may be an important issue for further investigations in the area of biochemical functional foods.
Wageningen University, The Netherlands
Title: The nutrient oxygen: challenge tests and metabolic health
Prof.dr.ir. Jaap Keijer obtained his MSc degree in 1985 from Wageningen University and his PhD in 1991 from the University of Amsterdam, the Netherlands. He was appointed full professor and chair of Human and Animal Physiology (HAP) at Wageningen University in 2008. Jaap Keijer is a molecular physiologist and an expert in the molecular response analysis of functional food components, in particular those that target energy and substrate metabolism. He has published over 120 international peer reviewed publications, was chair of COST Action MITOFOOD and is a WP leader of FP-7 BIOCLAIMS (BIOmarkers of metabolic robustness for CLAIMS on foods).
Nutrition importantly contributes to our health through long term effects on homeostasis. These effects are difficult to identify for two reasons. First, effects size of individual food components usually is small and second, health rather than disease is targeted. To improve sensitivity of analysis tests are implemented that challenge metabolism. Thus, the flexibility of metabolism to respond to a challenge is examined, rather than a steady state. Since metabolic flexibility is directly related to health, this is an effective manner to examine effects of functional food components on health status. I will present our data on non-invasive metabolic challenge studies. Two type of challenges are performed. First, the response to a glucose challenge using indirect calorimetry is used.The second type of challenge is a restriction of the amount of oxygen that is available, which represents a non-dietary nutritional challenge. In addition to parameters related to energy metabolism obtained by using indirect calorimetry, transcriptome and metabolome data are generated to evaluate effects. Data from dietary interventions in mice and humans will be shown.
Kobe Pharmaceutical University, Japan
Title: Recent advances in Vitamin K metabolism
Toshio Okano has completed his PhD at the age of 27 years from Osaka University, Osaka, JAPAN and worked for two years as a visiting scholar at Cambridge University, MRC Molecular Biology, and Cambridge, UK. He is the full professor of the Department of Hygienic Sciences, Kobe Pharmaceutical University. He has published more than 200 papers in reputed journals and has been serving as a board member of Kobe Pharmaceutical University and the Vitamin Society of Japan.
Pylloquinone (vitamin K1: K1) is a major form of dietary vitamin K, but the form of vitamin K that exists at the highest concentrations in tissues of animals and humans is menaquinone-4(vitamin K2: K2). Despite this great difference, the origin of tissue K2 had not been clarified until recently (Okano T, et al, J Biol Chem, 2008). We have demonstrated that K1 is converted to menadione(vitamin K3: K3) in the intestine, followed by delivery of K3 to tissues and subsequent conversion to K2 by UbiA prenyltransferase domain containing 1(UBIAD1) (Hirota Y, Okano T, et al, J Biol Chem, 2013) that has been recently identified as a novel human K2 biosynthetic enzyme (Nakagawa K, Okano T, et al, Nature 2010). K2 is known to act not only as a cofactor for -glutamyl carboxylase but also as a ligand for the steroid and xenobiotic receptor (Suhara Y, Okano T, et al, J Med Chem, 2012). In addition, K2 is known to induce apoptosis in tumor cells and suppresses oxidative damage to oligodendrocyte precursor cells, suggesting that K2 is an active metabolite of K1. To fully understand the function of K2, we have generated both conventional and organ-specific ubiad1 knockout mice. Conventional ubiad1 knockout mice died during embryogenesis. On the other hand, conditional ubiad1 knockout mice were alive several months after birth, and K2 was not detected at all in their gene-targeted organs. I will introduce our data related to phenotypes with organ-specific ablation of ubiad1 and discuss on physiological roles of K2.
Hospital Carlos Haya, Malaga
Title: Role of dietary fats in Ghrelin and GLP-1 metabolism
Francisca Rodriguez Pacheco completed her PhD in Biology in the year 2007 from University of Córdoba, Spain. She owns an 11 years of professional activity as researcher in molecular and Cellular Endocrinology. She also completed Post doctoral studies in Carlos Haya Hospital in Malaga, in the area of Endocrinology and Nutrition. She is a Professional member of the IBIMA Institute Biomedical of Málaga. She has participated in numerous congresses and published 11 papers in reputed journals and serving as an editorial board member of repute. Her main field of research is on Mediterranean Diet and its beneficial effect on diabetes and obesity.
Background and objectives:Ghrelin is a orexigenic peptide secreted primarily by the stomach. Its regulation by diet is not sufficiently well known and its relationship with other anorectic péptides. The overall objective of this work is to study human ghrelin and GLP-1 response to 3 breakfasts differ depending on the type of fatty acid consumed in the diet.
Methods:This study was conducted in 10 normal weight patients who have taken three different breakfast for 3 consecutive weeks, one in a week. The breakfast consisted of 50g of bread + 25cl of oil, three different types: extra virgin olive oil, refined olive oil and sunflower oil. Blood samples were collected before the intake (time = 0 min) and at 15, 30, 60, 90.120, 150 and 180 min after intake of food.
Results:Ghrelin secretion inhibition after meal is different depending on the type of fatty acid in the diet and this inhibition is simultaneous to the stimulation of the anorexigenic peptide as GLP-1. At 15 minutes from the intake of food there is a falling ghrelin levels that is els similar to the three types of fat ingested. But breakfast with extra virgin olive oil keeps ghrelin levels lower than any of the other two fats at 180 min of collection. The highest levels of GLP-1 are obtained at 15 min of intake started reaching the highest point when the patient eats extra virgin olive oil.
Conclusions:A diet enriched with olive oil increases the inhibition of ghrelin secretion and the increase of GLP1 secretion.
Acknowledgements:The partner company in the gift of olive oil has been GROUP HOJIBLANCA, who would greatly appreciate your cooperation. Obesity CIBER, CIBER of Diabetes and Metabolic Diseases (Ministry of Science and Innovation). Sara Borrell Program (Ministry of Education and Science).
University of Allahabad, India
Title: Addressing nutritional issues through mushroom biotechnology
Mohan Prasad Singh is presently working as Professor in Biotechnology at University of Allahabad (India). After completion of M. Phil. and qualifying NET he joined as Lecturer in HNB Garhwal University in Botany in 1992. In 1998 he completed his Ph.D. and in 2000 he moved to the Department of Biotechnology at VBS Purvanchal University, Jaunpur where he became Reader in 2000 and Professor in 2008. Prof. Singh has been carrying out his research on various aspects of edible oyster mushroom over last two decades. He has improved production and nutritional content of mushroom and suggested the effective way to get rid of environmental problems through this mushroom. He has published 21 research papers, edited three books and has been serving as an editorial board member of repute. His research area is Mushroom nutraceuticals, biodegradation & bioremediation.
The issues of population, production and nutrition are linked. Population explosion has led to multifarious problems in which food scarcity and malnutrition have drawn attention of scientists all around the globe. Acute malnutrition is glaring reality in developing countries because of unavailability or less availability of protein in staple food. Mushroom cultivation and mushroom biotechnology can be low cost, viable multipurpose technology to deal with malnutrition and related problems. Generally edible mushrooms possess all the three properties of food – nutrition, taste and physiological functions. They contain high quality protein that can be produced with greater biological efficiency. They are rich in vitamins, minerals and fibre and have low crude fat content with high amount of unsaturated fatty acids. The protein content of fresh mushroom varies from 1.75 to 5.9% with an average value of 3.5 to 4%. On dry weight basis mushrooms contain about 19-35% protein as compared to 7.3% in rice, 9.4% in corn, 12.7% in wheat and 38.1% in soybean. Besides, mushroom protein contains all the nine essential amino acids required by human beings. The amino acids content can be enhanced by applying the tool of mushroom biotechnology. By using the vegetable and agricultural wastes as supplements, protein content in fruit bodies become higher and essential amino acids show significant increase. Similarly, by adding sodium selenite to compost, the selenium content in mushroom fruit bodies can be enhanced which is an essential micronutrient and a necessary cofactor for glutathione enzyme system for removing free radicles from the body, thus, reducing oxidative damage.
IVF Centers Prof Zech, Austria
Title: Antioxidant supplementation of subfertile men improves top blastocyst rate in couples undergoing IVF/ICSI
Johannes Wogatzky studied medicine at the universities of Hamburg Heidelberg / Germany and Basel (Switzerland) and has completed his M.D. with a doctoral thesis about stress coping and arrhythmias at the age of 29 years from Heidelberg University/ Germany. He gathered medical work experience in Germany, Switzerland, England, New Zealand and Australia. After a degree in sportsmedicine, family medicine and acupuncture he became an O&G consultant in 2006 and has directed his focus of interest to reproductive medicine from 2008 as the team leader physician at the IVF Centers Prof Zech in Bregenz/Austria, specializing onandrology and lifestylefactors impacting reproductive wellness.
A man’s ability to father a child is closely associated to the quality of his semen. In subfertile men the use of antioxidant supplementation has been discussed as a means to improve semen quality. There are, however, only few studies assessing the impact of supplementation on fertility treatment outcome. In this study we present data on the treatment outcome of 92 couples undergoing IVF/ICSI. Semen samples were collected on their 1st cycle. During this cycle neither of the male patients had taken micronutritional supplementation.These data were compared with the 2nd cycle after the men have been taken an antioxidative supplementation (Fertilovit® M) for 3-6 months. Semen analysis were evaluated according to WHO and specific motile sperm organelle morphology examination (MSOME) criteria Additionally, the fertilization Rate (FR), Blastocyst rate (BR), top-blastocyst rate (tBR) as well as pregnancy- (PR) and ongoing pregnancy- (oPR) rates were assesed. In the total number of patients we found significant improvements of WHO sperm criteria after the intake of the micronutrient as well as an increase of class I spermatozoa according to MSOME criteria (p< 0.005) showing a clear impact of micronutriens even on the molecular level (vacuolization) of spermheads. Although the average age of the female patients had increased significantly (36.8 y. to 38.1 y. p<0.03), normally associated with a lowering embryoquality, we observed a rise in the BR after the men had been supplemented orally with the antioxidative preparation. The number of top-blastocysts increased significantly (p<0.05) We also found a rise in PR and oPR.
Farag Ali Saleh
King Faisal University,
Title: Antibacterial activity of date palm (Phoenixdectylifera L.) Fruit at different ripening stages
Saleh, A. F. has completed his Ph.D at the age of 32 years from Cairo University. He is the associated Prof of food science and nutrition. He has published more than 26 papers in reputed journals and serving as an editorial board member of repute. He is member in many professional societies. He has attended more than 15 national and international conferences and scientific symposium.
In vitro and in situ antibacterial activity of aqueous, ethanol, and ether extracts of three varieties of palm date (Khulase, Sheshi and Rezaz) in different maturation stages (Biser, Rutab and Tamer) were evaluated against some food borne pathogens. Gram positive bacteria showed higher sensitive against most extracts than Gram negative bacteria. Among the Gram positive bacteria, Listeria monocytogenesATCC 7644 and StaphylococcussaprophyticusATCC 15305 recorded highest sensitive against most extracts. The ethanol followed by water extracts of Biser stage had a stronger antibacterial activity than other maturation stage for all varieties against Staph.saprophytic. The results of minimum inhibitory concentration (MIC) shows that Staph.saprophyticus was very sensitive to the lowest concentration of ethanol extract of Rezaz variety at Biser stage (RBOH), (MIC, 3.75 mg/ml). RBOH was able to reduce the count of Staph.saprophyticus in minced camel meat by above of 1 log cycle during storage at 5ᵒC. This extract (RBOH) contained highest amount of phenolics (2035.3 mg/100g) compared to other extracts.
Z. O. Apotiola
Lagos State Polytechnic, Nigeria
Title: Baking and storage stability of Vitamin A in wheat flour
Mr. Zaccheaus Olasupo Apotiola holds B.Sc degree in Food Science and Technology from Obafemi Awolowo University and Msc in Food Quality Control/Quality Assurance from Federal University Agriculture, Abeokuta in 2007. He worked in standard organization of Nigeria from 1980 – 1996 and had about 15 standards to his credit. He joined Lagos State Polytechnic in 2001 and had published about 20 research works up to date.
The objective of the work was to determine the vitamin A level in two brands of wheat flour PWF and WWF stored at room temperature for six months and the flour was used to bake bread PBG and WBH. The vitamin A content of the flour and bread samples was carried out every month using high performance liquid chromatography. The vitamin A content of the flour samples reduced significantly with time. The PWF ranged from 12.84 to 1.09mg/kg and the WWF ranged from 9.96 to 1.13mg/kg. The vitamin A content of the bread sample also reduced significantly with time. The PBG ranged from 3.01 to 0.45mg/kg and WBH ranged from 2.57 to 0.64mg/kg.
Kouassi Kafui Codjo
University of Lome, Togo
Title: Protein-energy nutritional status of 103 cirrhotic patients versus 67 witness, hospitalized in campus teaching hospital’s hepato-gastroenterology service of Lome (Togo)
Kouassi has completed first, his four years biomedical study in the High School of biological and food of University of Lomé and after his Ph.D in the same University in collaboration with the biochemistry department of Dijon’s University (France). He has also the University of Lorraine’s (France) diploma of Quality Management and some certificates of the University of Cambridge’s Institute of Health Improvement (USA). He is the Responsible of Biochemistry department of the clinical laboratory of the teaching hospital of campus and had published in health nutrition field. He has participated at the ASLM French conference as a speaker.
The goals of this study are to evaluate the impact of the clinical severity degree of cirrhosis on the protein-energy nutritional status. Also the relationship between the patients lipids consumption and theirs nutritional state diagnosis by reliable stool as the Harpenden skinfold caliper. It is a case-witness transversal prospective study carried out between March 1st and September 15st, 2012 including 170 hospitalized adult patients. Other non-anthropometric parameters used were: serum prealbumin, albumin, PCR (Protein C reactive) and orosomucoïd. A week/24-hour recall dietary assessment method was used. The Child-Pugh classification was used to establish the clinical severity of liver disease. Forty nine patients and thirty seven other were respectively classified in group B and C according to Child-Pugh classification. 96% and 74% respectively of the patients in group B and C were severely undernourished according to the triceps skinfold thickness (TST). The prevalence of the under nutrition within the patients was 82% versus 52% of the witness (p < 0.05). About 90% of the subjects of this study didn’t consummated Poly-unsaturated fat acids by day. In the Child-Pugh group C 86% of the patient consummates lipids just one time by day and 87% of group A twice by day. Often the patients in group C are advised to do not consummate proteins. We must be awarded of the nutritional status of patients. Because of the injury of the liver, reliable parameters non-secreted by its, must be used to evaluate the protein-energy nutritional state and appropriate advice's should be given.
Moshood Abiola Polytechnic, Nigeria
Title: Health and hypertension status of civil servants in Abeokuta south west Nigeria
Nupo Sunday Sedodo is a seasoned nutritionist. He is currently a lecturer in the department of Nutrition and Dietetics in Moshood Abiola Polytechnic. He is a member of Nutrition Society of Nigeria and the General Secretary of the Ogun State Chapter of Nutrition Society of Nigeria. He has many publications in reputable journals both local and international to his credit. He had been a resource person in both local and international conference. He is happily married to Mrs Abosede Nupo and Blessed with beloved Israel of God.
This study aimed at determining the prevalence of hypertension among civil servants. A cross sectional study was carried out among 500 civil servants in Abeokuta. The blood pressures of the subjects were measured using sphygmomanometer. The results of the BMI showed that 4% were underweight, 54% had normal weight, 26 % were overweight, 16% were obese. WHR revealed that 78% had low risk of malnutrition. 12 % had moderate risk while 10% had high risk. Blood pressure measurements revealed that 20% of the subjects were at pre hypertension stage, 6% had stage 1 hypertension while 2% had stage 2 hypertension.
Track 3: Nutritional Deficiency & Disorders
Track 4: Nutritional Therapy & Treatment
Target Nutrition Therapy®, USA
Title: Target nutrition therapy® for eating disorders- Nutritional healing + brain chemistry balancing
Founder of Target Nutrition Therapy®, Christina Santini treats health a-z, specializing in the brain and biochemical rebalancing for different types of disordered eating behaviors and addictions. Christina Santini has worked at private hospitals for eating disorders and addictions in Denmark and The US, including brain health expert Eric Braverman, MD, developing nutritional programs based on analyzing blood labs in addition to interpreting the BEAM (Brain Electrical Activity Map). Christina Santini has a professional bachelor’s in Human Nutrition + Coaching from Metropol University Copenhagen and is a Naam Yoga Therapist from the Naam Healing & Research Center in Los Angeles.
Christina Santini will talk about what the unique brain chemistry imbalances are for various types of disordered eating behaviors. Focusing on how to treat these respective imbalances from a nutritional perspective to balance the biology behind the disease. During this session you will get tools from the trade on how to assess factors and lab markers that are connected to neurotransmitters. Neurotransmitters act like communicators and send off messages of health or disease – they also regulate our emotions incl. our eating behaviors and food preferences. We will explore the brain chemical factors for developing and maintaining a disordered eating pattern and how to break it. Christina Santini will go over cases and show how lab values, family medical history in addition to environmental and dietary triggers contribute to different types of disordered eating behaviors (Anorexia, Bulimia, Binge Eating Disorder and Food Addiction).
Following questions will be answered:
• How do I assess a client's brain chemistry based on family medical history, blood labs, eating behaviors and other behavioral abnormalities?
• What are the brain chemical traits of the different types of eating behaviors: Anorexia, Bulimia, Binge Eating Disorder + Food Addiction?
• Which nutritional strategies match the different brain chemical imbalances and how to implement them in order to still break the typical eating disordered black-white addictive thinking and food-phobic pattern?
• Which early intervention steps can be taken to prevent a genetic disposition for disordered eating from manifesting itself?
Aida A. Korish
King Saud University, Saudi Arabia
Title: Camel Milk Attenuates the Biochemical and Morphological Features of Diabetic Nephropathy: Inhibition of Smad1 and Collagen Type IV Synthesis
Prof. Aida A. Korish got her PhD degree from the College of Medicine- Alexandria University- Egypt at 2002. She is a Professor of physiology, College of medicine, Alexandria University. She is currently a visiting Associate Professor of Physiology, College of medicine, King Saud University. She has published more than 25 papers in reputed journals and has been serving as a global reviewer of many prestigious journals. She is interested in studying the blood biomarkers of renal diseases, the role of oxidant stress in renal diseases and the role of the alternative medicine in the treatment of chronic health problems.
Diabetic nephropathy (DN) is a common microvascular complication of diabetes mellitus (DM) that worsens its morbidity and mortality. There is evidence that camel milk (CM) improves the glycemic control in DM but its effect on the renal complications especially the DN remains unclear.
: Using STZ-induced diabetes, we investigated the effect of CM treatment on kidney function, proteinuria, renal Smad1, collagen type IV (Col4), blood glucose, insulin resistance (IR), lipid peroxidation, the antioxidant superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH). In addition renal morphology was also examined
Rats with untreated diabetes exhibited marked hyperglycemia, IR, high serum urea and creatinine levels, excessive proteinuria, increased renal Smad1 and Col4, glomerular expansion, and extracellular matrix deposition. There was also increased lipid peroxidation products, decreased antioxidant enzyme activity and GSH levels. Camel milk treatment decreased blood glucose, IR, and lipid peroxidation. Superoxide dismutase and CAT expression, CAT activity, and GSH levels were increased. The renoprotective effects of CM were demonstrated by the decreased serum urea and creatinine, proteinuria, Smad1, Col4, and preserved normal tubulo-glomerular morphology.
Beside its hypoglycemic action, CM attenuates the early changes of DN, decreased renal Smad1 and Col4. This could be attributed to a primary action on the glomerular mesangial cells, or secondarily to the hypoglycemic and antioxidant effects of CM. The protective effects of CM against DN support its use as an adjuvant anti-diabetes therapy
Massey University, New Zealand
Title: Dairy products in the global market: Naturally occurring opportunities to enhance animal and human health through an understanding of the functionality of dairy products
Jean is the Leader of Dairy Systems Research in the Institute of Agriculture and Environment, at Massey University. She completed a PhD and teacher training in the UK, and has been teaching and researching for over 20 years I the UK and now New Zealand, where she joined the Institute of Food, Nutrition and Human Health, specialising in Animal Nutrition and Lactation. Jean has held the posts of Major Leader of Animal Science, has published papers and book chapters in her specialized area, is a country representative on EU projects, sits on a number of committees, and both holds journal editorial and review posts.
New Zealand has a rapidly expanding dairy cattle population, currently standing at over 6.5 million, outnumbering the human population by 1.2:1. Over 90 % of the dairy products are exported, mainly as milk powder, butter and cheese, which supply the global commodity market, with an ever increasing portfolio of high quality and functional food markets. The national dairy herd consists mainly of cross bed (Friesian and Jersey) cattle, lowering the risk of milk protein gene mutation. Cattle are grazed already round on fresh pasture, increasing the concentration of functional fatty acids and CLA in milk. NZ soils are selenium deficient, however increasing global demand for milk has elevated milk price and greater profitability from intensification can be achieved and depends on supplemental feed quality, how well animals are managed and incorporated into the pasture system. This paper will discuss the areas of the dairy system within which pasture, supplementary feeds, forage quality and diet composition are used to increase milk product production and supply the expanding function dairy food market.
Ana Lívia de Oliveira
Federal University Juiz de Fora, Brazil
Title: Nutritional and systemic inflammatory activity in patients with colorectal cancer who underwent evaluation supplementation with symbiotic
Ana Lívia de Oliveira graduated in Nutrition in Federal University of Ouro Preto - UFOP, evidence of specialization in Clinical Nutrition by the Brazilian Association of Nutrition - ASBRAN, specialization in Human and Health Sciences at UFJF and Master of Health at the Federal University of Juiz de Fora - UFJF. She is currently professor of the Nutrition course UFJF. Experience in teaching, clinical nutrition, oncology nutrition, management of hospital food and nutrition consulting service.
Nutritional depletion is observed in patients with colorectal cancer in advanced stages, even mediated with appropriate weight. This loss may be associated with factors co - morbidities such as: reduction of increased immunity from infections, impaired wound healing and muscle weakness. The presence of malignant tumor stimulates the metabolic response to acute phase with increased positive proteins such as C-reactive protein. Currently a nutritional strategy used in cancer patients is the use of prebiotics and probiotics or a mixture of the two – symbiotic.
A prospective longitudinal study with a sample of 9 adult patients of both sexes with a diagnosis of colorectal cancer in the postoperative phase , undergoing cancer surgery and the use of symbiotic one sachet daily in the Outpatient Hospital was made Maria José Reis Baeta - ASCOMCER in Juiz de Fora MG - Brazil from May 2008 to May 2009 . Proteins such as C-reactive protein and albumin - anthropometric data and laboratory tests for the evaluation were collected. Data analysis was performed by means of descriptive and inferential statistics. The protocol was approved by the Ethics Committee of the Federal University of Juiz de Fora - MG under number 391/2007.
The results suggest a positive effect of the symbiotic on systemic inflammation in patients with colorectal cancer, in view of the lower proteins such as C-reactive protein levels at study completion. Seems to be a potential symbiotic on nutritional status by other terms and dosage. Further studies should address the use of symbiotic supplements in colorectal cancer.
Alvaro L. Ronco
IUCLAEH School of Medicine, Pereira Rossell Women’s Hospital, Uruguay
Title: Nutriequalization: Attempting to improve survival in cancer patients through nutritional modifications
Alvaro Ronco graduated as M.D. in Uruguay (1986) and worked at the Uruguayan National Cancer Registry (1987-2004), focusing research mainly on nutrition and cancer. He works on breast cancer at the Women’s Hospital (Montevideo, 2004- ). Associated Professor of Cancer Epidemiology (IUCLAEH School of Medicine, Maldonado, Uruguay, 2008-). Ronco is Opinion Leader in Breast Cancer Epidemiology at the S.I.S. (Strasbourg, 2003- ). Eight times awarded by the Uruguayan National Academy of Medicine, author/coauthor of >220 scientific works, (̴130 papers in international journals), he published Nutritional Epidemiology of Breast Cancer (Springer 2011), the first book in the world about it.
Despite the therapies, the effects of previous nutrition in cancer patients will remain in time, somehow facilitating the circumstances which enabled the tumor development. On the other hand, certain protective nutritional factors might also be effective in delaying tumor progression in an already diagnosed cancer.
In 2004-2013 many published studies showed the advantages of improving diet for patients diagnosed with some types of cancer, regarding the disease-free and the overall survival. There were also communications reporting an improvement when some nutritional guidelines were followed focusing on the management of overweight/ obesity. According to specialized literature, a healthy diet and lifestyle not only can inhibit carcinogenesis but they can also show a high impact on cancer progression and survival.
With the same aim, we propose a nutritional strategy in order to collaborate with the cancer therapy, based on several items which were drawn from selected data obtained in local case-control studies: intake of red and white meat, dairy foods, oils and fats, vegetables and fruits; fat-to-muscle ratio, and selected laboratory tests. Unlike these latter, the selection of items might represent population-specific features. Our objective is to perform a nutritional reprogramming in a tailored way, changing the exposure of the putative risk and protective factors to the lowest possible risk level. Since no studies indicate that a prudent dietary style is pejorative for health, we are trying to change an inadequate previous nutritional pattern into an adequate one in order to make feasible a change of the prognosis and quality of life.
Osama A. Fekry
Dar Alshefa hospital, Egypt
Title: Impact of nutritional modification on Egyptian patients with fatty liver combined with HCV
Was born in 1981, Cairo, Egypt. He works as the head of clinical nutrition department at "Dar Alshefa hospital" , one of the leading hospitals at the ministry of health , being the first formal clinical nutrition department , He had his master degree in Hepatology and Gastroenterology (Cairo university) in 2011 and his thesis was about " the impact of nutrition in living donor liver transplantation" which was the first of its type among the research in Egypt and won a prize from the (EASLGD) Egyptian Association for the study of liver and gastrointestinal diseases as the best research. His background as a Hepato-gastroenterologist affected his vision of clinical nutrition, Once he finished his ESPEN Diploma (European society of enteral and parenterl nutrition) in 2011, he founded the Fatty liver unit at the National Hepatology and Tropical medicine Research Institute (NHTMRI), to be the first and the only unit that deals with such a disease in a country that suffers from a burden of liver diseases. Now he is doing his medical doctorate in "Nutrition and liver cirrhosis".
Background: Egypt is the highest prevalence of Hepatits C viral infection all over the world. Estimated to be 14.7% of the whole population, on the other hand, the prevalence of obesity is getting higher, according to the WHO 70 % of the Egyptians are overweight or obese.
Fatty liver is observed in 75 % of obese persons and 35 % may progress to NAFLD.
Hepatic steatosis in hepatitis C virus (HCV)-infected patients has been shown to enhance the progression of liver fibrosis and decrease the response to antiviral Therapy. Multiple investigations are done to diagnose and grade the extend of fibrosis , liver biopsy is the most accurate , however many non-invasive parameters are suggested to trace the degree of fibrosis and its reversal like aspartate aminotransferase (AST)-to-platelet ratio index, AST/alanine aminotransferase (ALT).
Aim: Nutritional intervention is a must for patients of fatty liver and especially those of Chronic HCV planned for pegylated interferon therapy.
Conclusion: Nutritional intervention by means of reduction of body weight is reflected by improvement of the patient liver profile including non invasive parameters of fibrosis.
Universidade Federal de Pernambuco, Brazil
Title: Nutritional status early in life can permanently change brain functions
Angela Amancio-dos-Santos is graduated at Nutrition (1998), master at Nutrition (2001) and Ph.D. at Nutrition (2006). All her academic titles were held from Universidade Federal de Pernambuco, Brazil. During her Ph.D., she developed one year of training at Princeton University (Princeton, USA) and had her Post doctoral studies at Université Paris Descartes (Paris, France). She has experience in Nutrition, focusing on Malnutrition and Physiological Development, acting on the following subjects: Serotoninergic system, Central nervous system development, Cortical spreading depression.
Nutritional conditions early in life constitute one of the factors that can influence brain functioning. Initially, it is showed the effects of hypercaloric diet intake on brain electrophysiology. Wistar rats were suckled by dams fed a high-lipid (cafeteria) hypercaloric diet during the lactation period. After weaning, part of the pups remained in the high-lipid diet until adulthood (group ‘full-life’ FL), and the other part received the control (lab chow) diet (group L). A third group received the hypercaloric diet only at adulthood (group Ad). When the animals were adults, electrophysiology was analyzed. Brain excitability was reduced in the groups L and FL, but not in the group Ad, in comparison with a control group (C), fed the lab chow during the entire life. Electrophysiological changes observed in adulthood were associated with the hypercaloric dietary treatment during brain development. Secondly, neurogenesis was investigated in rats under dietary essential fatty acid (EFA) deficiency during development. Wistar rat dams received diets containing 5% fat either from coconut-oil (EFA-deficient) or soybean-oil (control). After weaning, pups received lab chow diet until adulthood. EFA-deficient group presented reduced body weight. Additionally, animals from both dietary conditions were treated with the antidepressive fluoxetine (10 mg/kg, i.p.) during three weeks. In following, their hippocampi were labeled to doublecortin (DCX), a marker of adult neurogenesis. The EFA-deficient fluoxetine group presented a higher number of DCX-positive cells in comparison with the control EFA-deficient saline group. Thus, EFA deficiency early in life persistently changes cerebral response to the fluoxetine.
Sultan Qaboos University, Oman
Title: From chemoprevention to identification of potential therapeutic targets and biomarkers for breast cancer
Somya Shanmuganathan is currently pursuing her third year PhD in Genomics of Breast Cancer at the Sultan Qaboos University, Oman. Prior to her PhD, she completed her Master of Business (Merit) at the Australian National University (Australia), Master of Biotechnology at the University of Queensland (Australia) and Master of Science in Applied Microbiology at the VIT University (India). She has been a reviewer for a number of articles in several reputed international journals and is currently involved in several breast cancer research works.
Over the years, several phyto-compounds have been extensively used in Complementary Alternative Medicine (CAM) studies, individually and often at higher doses to kill cancer cells. Based on the combination and synergism theory, we had previously demonstrated that a combination of Resveratrol and Indole-3-Carbinol synergized and killed a maximum number of Breast Cancer cells. In the present study, we have tested various combinations of 10 well known phytochemicals, used at bioavailable levels, for their effect on cell growth and proliferation of the MDA-MB-231; breast cancer (BC) cell line and MCF-10A; normal breast epithelium as control cell line. The results revealed a super combination of 7 phyto-compounds (7SC), that synergized and induced 100% clearance of the BC cells but did not affect the normal breast epithelial cells. Next, in order to understand the underlying molecular mechanisms of this ‘synergism’ effect, microarray analysis will be conducted on the mRNA collected from the 7SC treated and control cells at 12 and 24 hour time points. Ongoing in vivo experiments aim to evaluate the efficacy of the 7SC phyto-compound treatment in preventing tumor growth using xenograft mouse BC model, and further validate the functional relevance of these genes in BC cell growth and survival. The data supports our hypothesis that the 7SC could be used in CAM as a dietary supplement approach against BC, and further identify genes that have the potential to serve as biomarkers and gene candidate to guide the design of appropriate anti-BC therapeutic strategies.
Shahid Beheshti University of Medical Sciences, Iran
Title: Food groups intake and multiple sclerosis disease: A case control study
Bahram Rashidkhani has completed his PhD at the age of 35 years from Karolinska Institutet in Sweden. He is currently an Associate Professor in Shahid Beheshti University of Medical Sciences. He has published more than 35 papers in reputed journals and has been serving as an editorial board member of repute.
Multiple sclerosis (MS) etiology is unknown, but different studies support the role of nutritional factors as one of the important factors in MS etiology. To provide further information on the issue, we examined the relation between food group’s consumption and MS risk in a population-based case-control study. This case–control study was conducted in hospitals in Tehran city (capital of Iran). A total of 70 patients with MS (12 men and 58 women, aged 17–62 years) and 140 controls (matched for age and sex) underwent face-to-face interviews. Dietary data were assessed by 125-item semi-quantitative food frequency questionnaire. Multivariate logistic regression was used to estimate OR and 95% CI. After modifying the effects of confounding variables, People who consumed the highest amount of: Fruits, tomato and other vegetables had significantly a less chance of getting affected by MS disease, respectively; 68% (OR: 0.32; 95% CI: 0.13-0.79), 82% (OR: 0.18; 95% CI: 0.05-0.65) and 61% (OR: 0.39; 95% CI: 0.16-0.93). On the other hand, people with the highest level consumption of non-liquid oils and cola, had significantly a greater chance to get affected by this disease, respectively; 1.58 times (OR: 2.58; 95% CI: 1.05-6.33), 1.87 times (OR: 2.87; 95% CI: 1.17-7.02). The outcome results of this study support the possible effects of nutrition in preventing MS disease.