10^th World Congress on Nutrition & Food Sciences May 29-31, 2017 Osaka, Japan

Chien Hua Huang is a first year master student in the Food Science Department at National Pingtung University of Science and Technology. She has a great passion for food science and has the expertise in cell and animal experiments and biochemical techniques such as Western blotting. Chien Hua Huang is studying the protective effect of Corbicula fluminea extracts in RGM 1 cell against indomethacin induced gastric mucosal injury. She has published four posters, one of which was presented at the ISNFF International Conference in 2016

Tocotrienols and tocopherols, naturally occurring isoforms of vitamin E, are found abundantly in food such as palm oil, rice bran oil, corn, oats, barley, rye and wheat. Tocotrienols have four different isomers: α, β, γ and δforms. The present study was designed to evaluate the protective effect of tocotrienols against methylglyoxal (MG)induced cytotoxicity in human neuroblastoma SHSY5Y cells. In order to assess the protective effect of tocotrienols, αtocotrienol (αT3), γtocotrienol (γT3) and δtocotrienol (δT3) were administered to SHSY5Y cells for 2 hours prior to the induction of cytotoxicity by MG, and the cell viabilities of SHSY5Y cells were analyzed by MTT assay. The cell viability of SHSY5Y cells were reduced to 40% by 24 h treatment of 0.5 mM MG, and two hours pretreatment of αT3, γT3 and δT3 increased the cell viability to 75%, 53% and 82%, respectively. It is shown that T3 has protective effect on the neurotoxicity induction by MG. Among T3, δT3 has the best protective activity. The future work needs to be performed to elucidate the neuroprotective mechanism of T3.

Min Hyun Suk has her expertise in teaching and research in Community Health Nursing. Her main study involves figuring out the real health status and affecting factors, and developing strategies in health promotion among adolescents and elderly people.

Backgrounds: Adolescence refers to the psychosocial, emotional, cognitive, and moral transition from childhood to young adulthood. Adolescence offer health care providers unique opportunities for health promotion and preventive services. Purpose: The purpose of this study was to provide basic data for strategies in maintenance of optimal body weight among adolescents. Methods: This study was a descriptive study based on a secondary analysis of raw data from the 9th Korea Youth Risk Behavior Web based Survey completed in 2013. The survey has been conducted annually with a complex design to identify the current health status and health risk behaviors of Korean adolescents enrolled in middle and high schools. A total of 799 schools were participated in the survey, and, in total, 72435 students responded (response rate: 96.4%). The adolescents were divided into low weight, normal weight, overweight, and obesity groups according to body mass index (BMI). Differences in food intakes were analyzed. Results: In obesity group, breakfast skipping rate was highest (χ2=27.13, p<0.001). Fruits and vegetable intakes were lowest in low weight (χ2=177.79, p<0.001) and normal weight (χ2=26.43, p<0.001) groups, respectively. Low weight group significantly intakes more carbonated soft drink (χ2=42.24, p<0.001), fast food (χ2=40.59, p<0.001), ramen (χ2=314.29, p<0.001), and snacks (χ2=641.87, p<0.001) than other groups. Conclusions: It was expected that intake of fast food, carbonated soft drink, ramen, and snacks would be higher in overweight and obesity groups. However, the results of this study showed that the intakes of fast food, carbonated soft drink, ramen, and snacks were higher in low weight group in difference with usual expectation. Therefore in the development of programs or policies for maintenance of optimal body weight among adolescents, the real status of food intake should be carefully surveyed and considered.

Ki Rim Kim is a student and an affiliate of Kyungpook National University, South Korea

Nicotine is a major toxic component of tobacco used through cigarette smoking and has been recognized to result in oxidative tissue injury as an important risk factor for cardiovascular diseases, lung related diseases and cancers. Prunus persica fruit has been used for the treatment of degenerative disorders such as hypermenorrhea, dysmenorrhea and infertility in Asia region including China, Korea and Japan. In this study, we investigated the protective effect of Prunus persica fruit extracts (PPFE) against chronic nicotine induced tissue damages in animal model. Male ICR mice were injected intraperitoneally with nicotine (5 mg/kg body weight) and then orally administered with saline or PPFE (250 or 500 mg/kg body weight) once daily for 36 days. Chronic nicotine administration induced nephrotoxicity and hepatotoxicity in mouse serum. Nicotine also caused an increase in level of malondialdehyde (MDA) as a lipid peroxidation product, and decreases in glutathione (GSH) level and activities of antioxidant enzymes, including superoxide dismutase, glutathione peroxidase and catalase, in liver and kidney tissues. PPFE treatment, however, decreased MDA level, and recovered GSH level and activities of antioxidant enzymes. In addition, PPFE inhibited nicotine induced histological changes and nitrotyrosine expression in tissues. These results suggest that PPFE may protect against tissue damages chronically exposed to nicotine.

Yu Ri Kang is a Doctoral Student at Hannan University, Daejeon, Korea and her research productivity as a graduate student is outstanding. She had very balanced experiences on both study (Hannam University, Korea; Exchange program from Framingham State University, Framingham, USA, 2013 and 2015) and research field (number of manuscript and poster presentation). Her major study field is bioactive polyphenols from natural plant foods inhabited in Jeju and US area and emerging issues in modern Food Science studies, especially in US.

Onion (Allium cepa L.) is one of the most widely used vegetable as food or medicinal plant due to its health benefits. It has been reported that onion and its extracts have antibacterial, antidiabetic and antioxidant activity. In our previous study, we confirmed that inhibitory activities of some onion cultivars against rat intestinal α glucosidase and porcine pancreatic α amylase were increased by heat treatment process (121°C, 30 min). In this study, heat treated sample was purified by cation exchange and gel permeation chromatography. Its structural characteristics were investigated by nuclear magnetic resonance (NMR) spectroscopy (1H, 13C, H–H COSY, HSQC, TOCSY, and NOESY). Furthermore, we investigated anti hyperglycemic activity of heat treated Jeju onion (Allium cepa L.), which is one of the yellow onion species grown in Jeju (Korea), in normal Sprague Dawley (SD) rat model. Heat treated sample was purified by column chromatography using cation exchange resin to obtain a purified product of a powdered form. To confirm the structure and purity of the sample, purified sample was subjected to NMR analysis, LC MS/MS, and HPLC system. The structure was elucidated on the basis of extensive spectroscopic analysis, including 1D, 2D NMR and ESI MS. Non heated onion had no significant change in postprandial blood glucose levels compared to control group in normal Sprague Dawley (SD) rat model. Meanwhile, heat treated onion significantly decreased the postprandial blood glucose levels 8.7% at 30 min (p<0.01) when compared to control group. These results suggest that development of heat treatment process with onion may provide useful dietary strategy for managing postprandial hyperglycemia.

Seong Soo Roh His major is traditional Korean medicine, and he has lectured and studied pharmacology and toxicity in Korean medicine at Daeguhaany university. In addition to natural drug research, he is also focusing on research on functional food ingredients and natural cosmetic materials. Now he is the editor in chief of The Korea Association of Herbology and serves as an academic member and editor of internationally renowned journals. He has built this model after years of experience in research, evaluation, teaching and administration both in hospital and education institutions.

We conducted experiments about immunopotentiating with powder of silkworm as food and immunity promoting materials (Angelica Gigas Root, Eucommiae cortex, turmeric, cinnamon, Alpinia oxyphylla Miquel, Achyranthes bidentata Blume, liriope radix, Japanese lady bell, chives, ulmoides). In order to measure the effect of enhancing the immune function, a sample of silkworm powder and composite materials were orally treatment for 2 weeks and T lymphocyte proliferation and IL 2, IL 4, and IFN gamma secreted by T lymphocyte activation, degree of nitric oxide uptake and total spleen, lymphocyte, and peritoneal macrophage counts were measured in macrophages. That is, in vitro cytotoxicity was observed when the cells were co cultured in silkworm powder and immunoprecipitation candidate treatment for 24 hours. As a result among 10 kinds of immunopotentiating material, 100 ug/ml Eucommia ulmoides, 400 ug/ml, 200 ug/ml cinnamon, 100 ug/ml liriope radix, 100 ug/ml chives, and Acanthopanax 100 ug/ml were chosen based on the results of spleen cell proliferation ability. The control group treated with Concanavalin A (Con A) for activating splenocyte T cells and the control group treated with Con A and candidate material samples were simultaneously treated to determine whether synergic effect of T cell proliferation occurred. As a result, group treated with each combination extract of Silkworm and Liriope radix, Cinamori cortex and Eucommiae cortex showed a synergistic effect on splenic T cell proliferation compared to group only treated with Silkworm. In addition, the expression levels of cytokines were increased in the silkworm cinnamon, silkworm liriope radix , and silkworm Eucommia ulmoides treated groups compared with silkworm alone group. In conclusion, it can be concluded that an equal amount of silkworms and mosquitoes can be obtained to enhance the immunity enhancing effect

Mi Ri Park is a PhD candidate and has great experience on functionality of dairy originated foods including milk and probiotics for promoting human health. In recent, she is focusing on the molecular mechanisms of probiotic strain and its fermented products for anti aging activity. In addition, she has interests in central nervous system (CNS) function by modulating signaling pathways of the microbiota gut brain axis. She has taken a novel viewpoint in this review by specifically highlighting that the gut microbial composition may be a key player for various metabolic diseases, and this may thus have critical impacts on CNS function, with significant implications for brain function and behavior.

The osteoporosis primary definition is a skeletal disorder of post menopausal women or of old people. But, recent definition is, a systemic skeletal disease characterized by low bone mass and the micro architectural deterioration of bone tissue, with a consequent increase in bone fragility and susceptibility to fracture. Here, we focused on evaluating the noble functionality of fermented milk with selected probiotics for anti osteroporosis using in vitro and in vivo assays. Among selected probiotic bacteria, Lactobacillus plantarum B719 exhibited higher (p<0.05) proliferation in MC3T3 E1 osteoblast cells, and was thus selected for subsequent analysis, involving for the comparison of osteoprotective effects with L. plantarum B719 fermented milk product (FMP B719). Furthermore, the effects of FMP B719 were investigated with medically ovariectomized (OVX) rats at the age of eight weeks. Results also demonstrated that assessment items for bone health including bone mineral density (BMD) in FMP B719 group are significantly improved compared to that of OVX groups (p<0.05). Interestingly, metagenome analysis showed that the taxonomic composition at the phylum level showed a considerable alteration in fecal microbiota induced by FMP B719. Taken together, we proposed that FMP B719 could be a potential candidate for prevention of osteoporosis, improvement of bone remodeling as well as inhibition of bone resorption through influencing dynamics of gut microbiota.

Sang Don Ryu has great experience on detection and characterization of foodborne pathogens in food microbiology research. In recent, he focused on the prevalence and molecular pathway of various foodborne pathogens including Listeria monocytogenes, Staphylococcus aureus, Camphylobacter jejuni, Salmonella typhumurium, and pathogenic E. coli isolated from dairy foods and environments. Especially, his outstanding results may help monitor and control the spread of bacterial species through the farm environment, which is a natural reservoir for these organisms

The entry route of foodborne pathogens including EHEC O157:H7 from the dairy farm environment into raw milk and dairy foods may come from several different vectors, and influence on the human health. Here, we employed HT 29 intestine epithelial cells (IECs; in vitro) and C. elegans (in vivo) as host model systems and compared the specific host responses during EHEC O157:H7 infections using whole transcriptome analysis. To elucidate the immune pathways stimulated by EHEC O157, we employed quantitative real time polymerase chain reaction (qRT PCR), transgenic worms, and RNAi. After EHEC O157:H7 infections, the regulation of gene encoding detoxification process, stress response, immune response and oxidative stress associated proteins were immediately remodeled. Among them, we showed that the genes encoding cytochrome P450 (CYP450) family were dramatically induced by >10 fold during infection of EHEC O157:H7 is common in both host models. Importantly, C. elegans mutants lacking CYP450 genes were highly susceptible to EHEC O157:H7 infections comparing to wild type N2. Consistent with susceptibility tests, qRT PCR results showed that CYP450 loss of function mutations significantly affected the transcriptional induction of antimicrobial peptide genes such as clec 60. Taken in their totality our results provide critical insights for host strategy to exclude EHEC O157:H7 pathogenesis in gastro intestinal (GI) tracts through cytochrome P450 family.

Chun Jung Chen has devoted his effort in the study of viral encephalitis and neurological degeneration. By culturing primary neural cells and establishing diseased models from rodents, pathological mechanisms and therapeutic strategies are his interests. He has built many types of primary neural cells and diseased animal models of central and peripheral nervous system after years of experience in research, evaluation, teaching and administration both in hospital and university.

Statement of the Problem: Neuroinflammation has a central role in common pathologies of brain diseases. Japanese encephalitis, which is caused by infection with mosquitotransmitted Japanese encephalitis virus (JEV), is an example of an immunopathological disease closely associated with pathological disruption of the BBB and severe neuroinflammation. Those immunopathological changes correlate well with the onset and disease progression of Japanese encephalitis and represent promising targets for therapeutic control. With a view to gaining a better understanding of the pathogenic process and developing therapeutic options for Japanese encephalitis, further investigations of the underlying neuroinflammatory mechanisms during the course of JEV infection are needed. DHA possesses nutritional and pharmaceutical properties with a broad range of applications, including antiinflammation. Since dietary and nutraceutical supplements of DHA are increasingly becoming acceptable options for health promotion and/or therapeutic treatments, the purpose of this study was to investigate the effect of DHA on Japanese encephalitisassociated neuroinflammation. Methodology & Theoretical Orientation: Cultured neuron/glia containing 40% neurons, 40% astrocytes, and 20% microglia were prepared from cerebral cortices of SpragueDawley rats. JEV NT113, a JEV strain isolated from mosquito, was propagated in C6/36 cells and used to infect cultured neuron/glia. Findings: Infection of cultured neuron/glia with JEV caused a profound reduction of neuron viability and the neurotoxicity was attenuated by DHA. The neuroprotective action of DHA was accompanied by inhibition of NO, TNFa, and IL1b production. Mechanistic studies revealed that JEV infection elicited an elevation of tolllike receptor 7 (TLR7)/MyD88 signaling axis leading to activation of NFB and AP1 as well as expression of cytokines. We further identified that HMGB1 released from JEVinfected neuron/glia had neurotoxic effect and proinflammatory effect. In conclusion, current results indicate that DHA can attenuate JEV infectioninduced neurotoxicity involving resolution of neuroinflammation

Ji Yeon Kwak is an affiliate of National Institute of Food & Drug Safety Evaluation at Ministry of Food and Drug Safety in South Korea

Novel food ingredient means any food ingredient that has no history of consumption in Korea. Foods derived from sources not previously used as human foods must be evaluated for safety. Novel food ingredients scope is the agricultural, livestock, marine products and microorganisms newly introduced in Korea as well as the ingredients acquired from extraction, separation and cultivation of those materials. Since 2010, applicants are required under 'Temporary Standards and Regulations for food Ingredients (MFDS Notification No.2016-27)' to submit information to Ministry of Food and Drug Safety (MFDS) regarding the product (e.g., the status of approval and use in other countries, manufacturing methods, ingredient characteristics, toxicological test, evaluation of food intake, etc.) In March 2017, the record shows that there have been 20 cases that are approved as novel food ingredients including insects, plants, microorganisms, and sugars. Among the approved novel food ingredients, insects are Mealworm (Tenebrio molitor Linne.) larva, White worm (Protaetia brevitarsis) larva, Dynastid beetle (Allomyrina dichotoma) larva and Two-spotted cricket (Gryllus bimaculatus) and all sugars (6 cases) are allulose (liquid, powder) that is commercialized in Japan and the U.S.A. As a substance that exists in nature, allulose is found in small quantities figs and raisins, etc. Allulose has 70% relative sweetness to sucrose. The calorie of allulose is 0.2 kcal which is 5% lower than sucrose (4 kcal). Since 2016, Korea has continuously carried out national sugar intake reduction project and allulose will help reduce sugar intake as a low-calorie alternative sweetener.

Jia Lin Guo study in national pingtung university of science and technology masters degree program in food science, and he has expertise of HPLC analysis in natural compound.

Tangeretin (TAN), a kind of polymethoxyflavones (PMFs) found in citrus peels, has been proved to have many kinds of bioactivities including anti-inflammatory, anti-atherosclerosis and antitumor activities. Prostate cancer is universal malignant in male genital system. According to studies, 5-demethyltangeretin has hydroxyl group at the C5 position, and higher cytotoxicity than TAN for cancer cells, but the low solubility of 5-DTAN limits their availability in biological system. For the reason that we prepared 5-DTAN derivative (with acetyl group at the C5 position, 5-acetyltangeretin derivative; 5-ATAN), and hope to increase its solubility and bioavailability. The object of this study is to investigate the anti-proliferation and cellular uptake of 5-ATAN in PC-3 human prostate cancer cells. Esterase present in the cells, therefor, 5-ATAN will be converted to 5-DTAN when 5-ATAN was uptaken into cells. We could use high-performance liquid chromatography lined electrochemical detector (ECD) to detect concentration of 5-DTAN within PC-3 cell. In MTT assay, it demonstrated that 5-ATAN significantly inhibit growth of PC-3 cells in dose- and time-depend manners. In condition of HPLC-ECD showed that 300 mV is the best volt, and retention time of 5-DTAN is 11.6 minutes, and linear regression is 0.1-10 µM. Moreover, the quantification and detection limits (LOQ and LOQ) of 5-DTAN were 7.16 and 1.79 μg/ml, respectively. In bioavailability test, intracellular concentration of 5-DTAN of 5-ATAN-treated group was significantly higher than those of 5-DTAN-treated group, which showed dose- and time-depend responses. Overall, the results indicate that 5-ATAN showed highest anti-proliferation activity than TAN & 5-DTAN in PC-3 cancer cells, and had greater bioavailability than 5-DTAN. 5-ATAN will be expected to have better oral bioavailability in vivo.

He is Pu Hua Wu, Taiwanese. He is a second year master student at Department of Food Science in National Pingtung University of Science and Technology. As its name, it is located at Pingtung, Taiwan, pure and simple counties. He was born and grown in Pingtung. So He has more emotion not only Pingtung but this school

In recent years, tempe (tempeh) is being more attention than before. Tempe (tempeh) originates from traditional food of Indonesia, is produced by termentation of soybean. Traditional tempeh only uses Rhizopus to ferment. Trying Lactobacillus co-culture with Rhizpous to create more comfortable condition for fermentation. Diabetes, an increasingly serious disease of modern civilization. A healthy body secretes insulin to regulate blood glucose by pancreas. Through the action of insulin so that glucose in the blood transport into cells to provide energy which cells need. When the level of insulin is not enough or inefficient use of insulin to reduce blood sugar in body, the problem of high blood sugar will occur. The most common diabete is type II, which is acquired diabetes. It is confirmed that aglycone can help regulation of blood sugar. Tempe has more aglycone than other soybeans product. This study observes changes of blood sugar in diabetic rats by fed tempe. To induce diabetes uses pharmacy with high-fat diet. Using streptozotocin (STZ) with nicotinamide induces rats to get diabetes and make sure their blood sugar above 150 (mg/dl). At same time, rats had fed high fat diet which include cholesterol, coconut oil and normal diet. Comparing tempe, tempe with Lactobacillus are useful with negative control and soybeans groups. Obviously, diabetic rats regulation of blood sugar with tempe are very useful.

Yu-Kuo Chen has received his PhD from the Institute of Food Science and Technology of National Taiwan University in 2009. After completing around two years as a Post-doctoral Associate in the Department of Chemical Biology at Rutgers University, he joined the faculty at National Pingtung University of Science and Technology as an Assistant Professor in the Department of Food Science. His research focuses on the biological evaluation of functional foods, purification and identification of active components in functional foods and analysis of metabolism and bioavailability of those compounds.

Our previous results demonstrated that 3-hydroxypterostilbene (OHPt) has higher anti-tumor activity than pterostilbene (Pt) in vivo. In the present study, we would like to elucidate whether the stronger biological activity of OHPt is due to its higher bioavailability than Pt. We performed pharmacokinetic studies in male Sprague Dawley rats. The animals received a single oral administration of OHPt and Pt at the dose of 50 mg/kg through oral gavage. After oral administration, a series of plasma samples were collected at 0, 5, 10, 15, 30, 60, 120, 180, 240, 480 and 600 min for further HPLC analysis. The results showed that two unidentified metabolites without parent compound OHPt were observed following ingestion of OHPt; while only one metabolite (glucuronide/sulfate conjugates) and parent compound Pt were detected in rat plasma after ingestion of Pt. These results indicated that the biotransformation of OHPt might not be glucuronide or sulfate conjugation, whereas, the major biotransformation pathway of Pt might be glucuronide or sulfate conjugation. The findings of this study provide message that despite structural similarity, Pt and its hydroxyl analog OHPt exert completely differential effects on oral bioavailability and metabolism. Further experiment is needed to identify the unknown metabolites of OHPt.

HPLC fluorescence detectionï¼›LC MS MS The identification and analysis of three anti oxidants including: trans resveratrol, quercetin and emodin in grape and red wine have been studied by HPLC fluorescence and LCMS/MS detection with SFE in this work. Transresveratrol (trans3,5,4 trihydroxystilbene,resveratrol) is common found in grape and red wine. Transresveratrol (transRSV) has important effects on lipid metabolism and can be used as antiinflammatory, antioxidant, free radical scavenging, and antiallergy. It can also prevent the cardiovascular diseases. Besides, transRSV is also an effective cancer preventive medication. It can effectively inhibit tumor growth and cancer formation. Transresveratrol is sensitive to light and heat. Consequently, transRSV will convert to inactive cisRSV. The coexistence of antioxidants will also cause activity recession. In this work, quercetin (2(3,4dihydroxyphenyl) 3,5,7trihydroxy4Hchromen4one) and emodin (1,3,8trihydroxy6methylanthracene 9,10dione are also studied for their interferences in the analytical procedure for transRSV. The sensitivity and recovery for the proposed method for natural trans resveratrol from grape and red wine are also evaluated.

Ching Yi Kuan is a first year master student in the department of Food Science of National Pingtung University of Science and Technology in Taiwan. She has a great passion for food science. She can do cell experiments, Western blotting and animal experiments. She is studying the inhibitory activity of the stilbenes on liver cancer. She has published one poster in Taiwan Association for Food and Technology in 2015

According to the Ministry of Health and Welfare in Taiwan, prostate cancer is the sixth highest cause of cancer-related death. Besides, prostate cancer cells will metastasize to bones and lymph nodes, so it’s important that prevention and treatment of prostate cancer. In this study, 3'-hydroxypterostilbene (OHPt), a structural analogue of pterostilbene (Pt), were used as experimental samples to investigate the potent anti-tumor effects in PC-3 xenografted nude mice. 1×106 PC-3 cells were injected subcutaneously into the flanks of 30 male Balb/c nude mice to form tumors. When xenografted tumor volume reached around 100 mm3, mice were randomly divided into five groups. Each group consisted of 6 animals. Mice were orally administrated with daily doses of OHPt (5, 25 and 50 mg/kg/d) and Pt (25 mg/kg/d) for 4 weeks; while control group was orally administrated with olive oil only. During the experiment, the tumor volume was determined and recorded once or twice per week using caliper measurements. At the end of the experiment, animals were sacrificed by CO2 asphyxiation and the individual tumors were excised immediately and weighed. Further the protein expression of tumors was measured. The results showed that the treatment of mice with OHPt could inhibit the tumor volume and tumor weight in a dose-dependent manner, and increased the expression of c-caspase-3 while decreased the expression of MMP-9 and Cox-2. In conclusion, these results revealed that OHPt might be a potential agent to treat prostate cancer.

Diana Mareta IFA Pitaloka, graduated from nutrition and health of Brawijaya University, is a master student of Taipei Medical University (TMU), Taiwan. She got basic knowledge about clinical nutrition, food science, and community nutrition at bachelor degree. From three of them, she was interested in clinical nutrition and food science excellently.At the master program, she concern about high fat diet, inflammation, and lipid metabolism. Recently, she learn about glutamine and arginine against lipid metabolism in the liver.

Introduction: Status epilepticus is a condition which has seizures more than 30 minutes without recovery between them. Seizures in the meningoencephalitis patients are caused by enhancement of emphasis and expansive of inflammation area in the brain. The ketogenic diet was given to the epileptic patient to minimize the seizures through some pathways such as: inhibit neurotransmitter release and activation of ATP-sensitive potassium channels, enhance overall bioenergetics and reduce oxidative stress, and modulate tricarboxylic acid flux. Objection: The aim of this case study is to know the effectiveness of ketogenic diet in order to decrease seizures in a patient with status epilepticus. Method: This study was a case study which observed a patient (boy, 10 years old) at the Dr. Saiful Anwar Hospital, Indonesia, with status epilepticus and loss of consciousness. The ketogenic diet was given gradually, for 7 days (February 25th- March 3th, 2015), a patient was given pan enteral diet 1200 cc/day (1200 kcal, protein 36.72 g, fat 61,8 g, and carbohydrate 130.8 g), for 9 days (4th-12th March 2015) received ketogenic diet 1600 cc (2169.9 kcal, protein 16.32 g, fat 107.2 g, and carbohydrate 280 g). Result: Seizure decreased at the first day after ketogenic diet administration (seizures <30 minutes) and stop completely at second day after administration. Based on monitoring result, a patient had decreasing body weight and MUAC during ketogenic diet administration. Furthermore, patient had enhancement of lipid profile (total cholesterol 211 mg/dL, TG 256 mg/dL, HDL 32 mg/dL, dan LDL 146 mg/dL). Conclusion: The ketogenic diet which given gradually can reduce and inhibit seizures in the meningoencephalitis patient.

Taejoon Kang received a B.S. in chemistry from KAIST in 2004 and a Ph.D. in chemistry from KAIST in 2010. He did postdoctoral research at KAIST before joining in KRIBB as a senior researcher in 2012. He is currently senior researcher of BioNanotechnology Research Center and BioNano Health Guard Research Center in KRIBB and Associate Professor in major of Nanobiotechnology and Bioinformatics at UST

Rapid, specific and sensitive detection of pathogenic bacteria is crucial for public health and safety. Bacillus cereus is harmful as it causes foodborne illness and a number of systemic and local infections. We report a novel phage endolysin cell wall-binding domain (CBD) for B. cereus and the development of a highly specific and sensitive surface plasmon resonance (SPR)-based B. cereus detection method using the CBD. The newly discovered CBD from endolysin of PBC1, a B. cereus-specific bacteriophage, provides high specificity and binding capacity to B. cereus. By using the CBD-modified SPR chips, B. cereus can be detected at the range of 105–108 CFU/ml. More importantly, the detection limit can be improved to 102 CFU/ml by using a subtractive inhibition assay based on the pre-incubation of B. cereus and CBDs, removal of CBD-bound B. cereus, and SPR detection of the unbound CBDs. The present study suggests that the small and genetically engineered CBDs can be promising biological probes for B. cereus. We anticipate that the CBD based SPR-sensing methods will be useful for the sensitive, selective, and rapid detection of B. cereus.

Ponpis Raungkhajon is a Senior Professional Level Nutrition Officer at Prince of Songklanagar in Hospital located in Songkla, Thailand.

In a study of 224 patients, comprising 157 male patients and 57 female patients, to determine the correlations between Body Mass Index BMI and the risk of malnutrition, 124 patients were found to have BMI of 16.00-18.49 Kgm2 while 54 patients were found to have BMI less than 16.00 Kgm2. Using the same sample, an assessment of nutritional status of the patients using the Nutrition Alert Form shows that 78 patients had severe malnutrition while 112 patients had moderate malnutrition. In addition, by monitoring the change in patients’ weight over the course of 4 weeks, the study shows that 69 patients or 30 percent of the total sample experienced over 5% weight-loss, 36 patients or 16 percent experienced 2 5% weight-loss, and 108 patients or 48.2 percent experienced 1 2 % weight-loss. 160 patients had low Albumin serum with 143 patients having 2.0 - 3.5 mg% of Albumin serum and 17 patients having less than 2.0 mg% of Albumin serum. Patients received Enteral Nutrition Support according to Nutrition Care Process for Cancer Treatment Plan with 115 patients or 51.3 per cent of the total sample receiving 1.2 kcal1kdd and 15-20 gm protein, 66 patients or 29.4 per cent receiving 1.25 kcal1kdd and 10 - 15 gm protein, 31 patients or 13.8 per cent receiving 1.5 kcal1kdd, and 12 patients or 5.5 per cent receiving 1 kcal1kdd and 20 - 40 gm protein. Our patient care team is a multidisciplinary patient care approach designed to implement a mixed and integrated patient care system. In particular, the pre-treatment nutritional care is central to the reduction of risk of malnutrition and allows quick and uninterrupted access to nutritional care through our Care Process.

Chien is an assistant professor of food science, nutrition, and nutraceutical biotechnology at Shih Chien university in Taiwan. His research field included food analysis and bioassay especially focus on anti-cancer, anti-inflammatory, and analgesic effects by phytochemicals from food (Chien & Wang, 2016). Recently, he developing the anti-aging model to elucidated the protective effects of medical food on degenerative diseases such as osteoarthritis, sarcopenia, and neurodegeneration

As the world aging rapidly, neurodegenerative diseases became one of the most serious age-related health problems. In south Asia, several medical foods have been used as an anti-aging remedy since ancient times. Cordyceps (Paecilomyces hepiali mycelium), a traditional tonic, has been reported with various pharmacological activities. In this study, we utilized Drosophila model system mimicking Huntington’s disease (HD) to exam the neuroprotective effects of the ethanol extraction of P. hepiali mycelium (PHE). These HD flies are expressing motor dysfunction and early death. Results demonstrated that diet containing 1 mg/mL PHE could significantly prolong the mean lifespan up to 31% of flies by compared to the control diet. It was also found that PHE could improve the locomotor activity of flies at the young age (4 days). Besides, oxidative stress plays a causal role in the neurodegenerative process. PHE exhibit antioxidative properties in a concentration-dependent manner by using the DPPH radical scavenging and ferric ions reducing power assay. These results indicated that supplementation with PHE improved movement performance and longevity might contribute by its neuroprotective antioxidants. We suggest that the PHE can be developed as a potential dietary supplement against neurodegenerative diseases such as HD

Pakjira BenyaPanya is a Senior Professional Level Nutrition Officer at Prince of Songklanagarin Hospital located in Songkla, Thailand.

Objectives: To study and develop recipes from soy and soy-based products for female patients of OBGYN clinic at the Prince of Songkhla University Hospital who are in their menopause Methodology: Developing 4 recipes from soy and soy-based products Result: Southern Coconut-Rice with Chicken Curry provides the highest level of energy at 460 Kcal, followed by Stuffed Glutinous Rice Balls at 335 Kcal, Spicy Tofu at 270 Kcal, while Baby Mushroom provides the lowest level of energy at 190 Kcal. In addition, a look at how patients assess the recipes shows that the effectiveness of the recipe in controlling underlying medical conditions ranks highest with an average score of 4.43, followed by taste at 4.31, and presentation/appeal at 4.17. The assessment criteria with the lowest score was the ease of cooking, which only received 3.95 on average. The recipes were then assessed on 3 subsequent assessment criteria with the following results: Taste: Stuffed Glutinous Rice Balls scored highest at 4.6, followed by Spicy Tofu at 4.3, and Coconut-Rice with Chicken Curry at 4.25. Baby Mushroom scored lowest at 4.1. Presentation/Appeal: Spicy Tofu scored highest at 4.47, followed by Stuffed Glutinous Rice Balls at 4.2, while Coconut-Rice with Chicken Curry and Baby Mushroom received an equal score of 4. Ease of Cooking: Spicy Tofu score highest at 4.2, followed by Coconut-Rice with Chicken Curry at 4, while Baby Mushroom and Stuffed Glutinous Rice Balls received an equal score of 3.8.

Nindy Sabrina is a second year master student in the School of Nutrition and Health Sciences, Taipei Medical University, Taiwan. Nindy originally from Malang, Indonesia. She received her undergraduate education at Brawijaya University, Indonesia, with a degree in Nutrition and Health. Currently, she is actively involved in Indonesia Student Association in Taiwan. Nindy is broadly interested in human nutrition research particularly obesity, iron, and advanced glycation end products. In her master research, she is seeking to understand the relationships between dietary intake, serum iron biomarkers and advanced glycation end products in Non-Alcoholic Fatty Liver Disease (NAFLD) patient in Taipei

Background: Recent evidence suggests that calorie restriction (CR) is more effective for obesity treatment that dietary fat restriction. Meal replacement (MR) also have shown as an effective strategy for the long term maintenance of weight loss compared with a traditional reduced calorie diet regimen. However, it is not clear whether combination between calorie restriction with meal replacement diet (CRMR) would promote favorable change in body weight and body composition. The aim of this study was to compare the effects of dietary intervention focusing on either calorie restriction alone or calorie restriction plus meal replacement on weight loss and body composition. Subjects/Methods: The 99 eligible subjects were randomized to either 1500-kcal CR and CRMR groups for 12 weeks. The CRMR group was supplied with low-calorie nutrition drink mix powder. The changes in anthropometry and body composition were evaluated. Body composition was measured with dual energy x-ray absorptiometry (DEXA). Results: Seventy subjects have completed the study. During weight loss period, body weight and body mass index (BMI) decreased similarly (P<0.001) in the CRMR group (-7.39 ± 5.09 %;-7.40 ± 5.10 %, respectively) and CR group (-2.31 ± 2.07 %; -2.31 ± 2.09 %, respectively). Waist circumference decreased similarly (P<0.001) in the CRMR and CR groups (-6.80 ± 7.59 % and -5.96 ± 4.80 % respectively). Fat mass decreased more (P<0.001) in the CRMR group (-15.99 ± 9.37 %) versus the CR group (-4.20 ± 6.82). Android fat and gynoid fat decreased similarly (P<0.001) in the CRMR group by -7.44 ± 7.82 % and -3.26 ± 7.16 %, respectively. Conclusion: These findings suggest that CRMR is effective to body weight and body composition of obese in comparison with CR alone

Yi-Zhen Chen is a second year master student in the Department of Food Science at the University of National Pingtung University of Science and Technology. She obtained her bachelor's degree in Food Science at NPUST in June 2015. She's full of great interests in the knowledge about food science. For her Master’s Thesis subject is the regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters. She has published one peer-reviewed research article in SCI international journal and five conference posters, and she has passed the required qualification examination for six technician certificates.

Heart disease and cerebrovascular disease are the second and third leading cause of death in Taiwan in 2015. Many studies showed that the most important factor leading to cardiovascular-related diseases is atherosclerosis, and the main caused by atherosclerosis is high blood cholesterol levels. Over the years, the risk of cardiovascular disease associated with population continues to increase, so the healthy diet and exercise have become one of the important issues. Djulis (Chenopodium formosanum) is a native cereal plant in Taiwan, and the djulis hull (DH) was found to be its major source of antioxidant compound. Studies showed that it possesses several active components with hypolipidemic activities, such as dietary fiber and polyphenol compounds. The objective of this investigation was to evaluate the regulation effects of DH in blood lipid levels to achieve the prevention of atherosclerosis effect. Five weeks old hamsters were randomly divided into five groups: (1) control group, (2) hyperlipidemic group (high fat diet containing 0.2% cholesterol; HFC), (3) low-dose DH group (HFC diet and treated with 100 mg/kg/day DH; LDH), medium-dose DH group (HFC diet and treated with 250 mg/kg/day DH; MDH), high-dose DH group (HFC diet and treated with 500 mg/kg/day DH; HDH). After 8 weeks of feeding study, the results showed that levels of triglyceride (TG; LDH: 194±31 mg/dl; MDH: 194±25 mg/dl; HDH: 204±26 mg/dl), total cholesterol (TC; LDH: 328±25 mg/dl; MDH: 301±21 mg/dl; HDH: 283±41 mg/dl) and low-density lipoprotein cholesterol (LDL-C; LDH: 165±33 mg/dl; MDH: 145±31 mg/dl; HDH: 140±28 mg/dl) in serum were significantly reduced by DH administration compared with those of HFC group (TG: 309±64 mg/dl; TC: 398±79 mg/dl; LDL-C: 184±31 mg/dl). Moreover, levels of TG and TC in liver were lower than HFC group, and levels of TG and TC in feces were higher than HFC group. These results indicate that DH possesses hypolipidemic potential to be a benefit functional food to prevent high fat/high cholesterol diet-induced hyperlipidemia.