Day 2 :
Keynote Forum
Jiankang Liu
Professor and the Dean of Xi’an Jiaotong University, China
Keynote: Nutritionally targeting mitochondria to prevent age-associated diseases: Hydroxytytrosol is a rising stars after resveratrol
Time : 9:30 - 10:10

Biography:
Dr. Liu received his BS from Xi’an Jiaotong Unviersity in 1982 and PhD of Medical Science from Okayama University School of Medicine in 1994. He completed post-doc training in Dr. Bruce Ames laboratory at University of California, Berkeley and worked as a faculty at University of California at Berkeley, Children Hospital Oakland Research Institute, University of California at Irvine, University of Kentucky College of Medicine, and Shanghai Institute for Nutritional Science, Chinese Academy of Sciences. Currently, he is a Professor and the Dean of Xi’an Jiaotong University School of Life Science and Technology and an Adjunct Professor of the School of Pharmacy, University of Southern California. Dr. Liu’s research interests include molecular and cellular mechanisms of aging, stress, and age-/stress-associated degenerative diseases with a focus on nutritional regulation of mitochondrial metabolism. He has published more 170 papers in international journals with more than 5200 times citations (H-index 42)and was elected as one of the “2014 , 2015 and 2016 Most Cited Chinese Researchers” in the area of Biochemistry, Genetics and Molecular Biology by Elsevier.
Abstract:
Due to the limitation and ineffectiveness of antioxidants in extending life span and preventing age-related diseases, scientists have being tried to find new solutions beyond antioxidants for delaying aging and preventing age-related diseases. The French paradox-derived resveratrol has become a star of nutraceutical because of the discovery of its effect on Sirtuins regulation by Sinclare’s lab at Harvard on. Nowadays, resveratrol has been widely studied on retarding aging and preventing/ameliorating numerous disorders in cellular and animal models as well as clinical studies. We have recently investigated a number of naturally occurring nutraceuticals and found that hydroxytyrosol (HT) has a unique and potent effect on regulating mitochondrial metabolism (biogenesis and degradation). HT is abundant in olives and virgin olive oil, which are widely used in Mediterranean diets. Epidemiologic studies have demonstrated that the people of the Mediterranean basin enjoy a healthy lifestyle with decreased incidence of heart disease, prostate and colon cancer, and rheumatoid arthritis. One of the possible reasons is that Mediterranean people have a high intake of HT from olive and olive oil. The beneficial effects of HT have been mainly attributed to its antioxidant effect. However, we have demonstrated that HT is an effective mitochondria-targeting nutraceutical in various cellular and animal models, including age-associated macular degeneration, metabolic disorders, cancer, osteoporosis, and muscle atrophy. Because mitochondrial dysfunction has been implicated in aging and various diseases e,the potent effects of HT on regulating mitochondrial metabolism suggest that HT is a rising star of nutraceutical after resveratrol and will be widely used for delaying aging and preventing/ameliorating age-associated diseases.
- Plant & Livestock Nutrition | Human Nutrition & Dietetics | Dietary Supplements & Functional Foods | Food Science & Chemistry | Nutrition, Health & Aging
Session Introduction
Nilva Kazue Sakomura
Universidade Estadual Paulista “Júlio de Mesquita Filhoâ€, Brazil
Title: Optimal tryptophan intake for laying hens
Time : 11:50-12:20

Biography:
Nilva Kazue Sakomura has completed her PhD at Federal University of Viçosa and postdoctoral studies at University of Arkansas (USA) and University of KwaZulu-Natal (RSA). She acts as professor at the Paulista State University "Júlio de Mesquita Filho”, FCAV/UNESP and coordinates the Laboratory of Avian Sciences at same university. She participated in the elaboration of the last edition of Brazilian Tables for Poultry and Swine. Her research area is mainly foccused on modeling amino acid requirements of broilers, laying hens and broiler breeders.
Abstract:
The research was conducted to re-evaluate the assumptions of ideal ratios (IAAR) of the essential amino acids for Hy-line W-36 laying hens, using the amino acid deletion and nitrogen balance methods. The nitrogen balance trial was performed from 28 to 32 weeks of age, 12 treatments with 8 replicates and 1 bird per cage were used. A balanced diet (BD) was formulated to meet the IAAR and the requirement of other nutrients. The limiting diets were formulated diluting BD with corn starch and refilled with synthetic amino acids (AA) and other feed ingredients, except for the AA studied. The trial lasted 25 d, being the first 5 d for adaptation and the other 20 d for total collection of excreta and eggs. Diets, excreta and eggs samples were analyzed for nitrogen content to quantify nitrogen intake and excretion. From these data, nitrogen retention (NR) was calculated. The estimated requirement of each AA (AAR) was calculated as AAR= 100 x (1-NRi/NRcontrol) where NRi is the NR associated with each limiting diet calculated and NRcontrol is the NR associated with the balanced control diet. The estimated optimum ratio between each AA and Lys [(AA/Lys) * 100] and the optimum level of each AA (values in parentheses) were: Lys = 100 (0.673), Met+Cys = 82 (0.552), Thr = 69 (0.464), Trp = 21 (0.141), Arg = 104 (0.700), Val = 91 (0.612), Ile = 78 (0.525), Leu = 121 (0.814), Phe+Tyr = 119 (0.801), Gly+Ser = 77 (0.518) and His = 29 (0.195).
Wan Aidah Wan Ibrahim
Research Officer with Malaysian Cocoa Board
Title: Comparison of Sensory and Physical Properties of Sucrose Free Milk Chocolate Containing Different Types of Polyols.
Time : 12:20-12:50

Biography:
Wan Aidah Wan Ibrahim has a degree in Bachelor of Science (Hons) in Food Science from University of Leeds, U.K and a Master of Science in Food Technology from University Kebangsaan Malaysia. She is currently working as a Research Officer with Malaysian Cocoa Board in Cocoa Downstream Technology division.
Abstract:
Polyols are commonly used to replace sucrose in the production of chocolate that is low in calorie, tooth friendly and suitable for diabetics. Three types of polyols; isomalt, maltitol and erythritol, as well as sucrose were used in pilot plant scale production of milk chocolate. Sensory evaluation was carried out using quantitative descriptive analysis by 10 trained panelists. Result showed that replacing sucrose with different types of polyols did not significantly (p≥0.05) affects the cocoa taste, sweetness, milky taste, smoothness and melting behavior of the chocolates in the mouth. Consumer test from 100 respondents indicated that chocolate with isomalt and maltitol were equally preferred while chocolate with erythritol was less preferred mainly due to the cooling sensation detected in this sample. The processing parameters used in this study were able to produce sucrose free chocolates with comparable particle size distribution to chocolate with sucrose (measured by Malvern particle size analyser, UK). Hardness analyses measured at 24°C using an SMS Texture Analyzer (TA.XT plus, UK) showed that freshly made milk chocolate with maltitol and isomalt were percieved to be significantly (p≤0.05) harder to break compared to chocolate with erythritol and sucrose. Chocolate containing isomalt and maltitol displayed a quite similar rheological behavior (by HADV 11+ Brookfield viscometer, UK) and chocolate with erythritol showed a closer rheological property to that of sucrose. Color (by Hunterlab MiniScan XE Plus, USA) and surface gloss (by Sheen Tri-microgloss, UK) analyses showed that the physical appearance of chocolates with polyols were fairly stable when stored at chill temperature (16±1°C).
Wan Aidah Wan Ibrahim
Research Officer with Malaysian Cocoa Board
Title: Comparison of Sensory and Physical Properties of Sucrose Free Milk Chocolate Containing Different Types of Polyols.
Time : 12:20-12:50

Biography:
Wan Aidah Wan Ibrahim has a degree in Bachelor of Science (Hons) in Food Science from University of Leeds, U.K and a Master of Science in Food Technology from University Kebangsaan Malaysia. She is currently working as a Research Officer with Malaysian Cocoa Board in Cocoa Downstream Technology division.
Abstract:
Polyols are commonly used to replace sucrose in the production of chocolate that is low in calorie, tooth friendly and suitable for diabetics. Three types of polyols; isomalt, maltitol and erythritol, as well as sucrose were used in pilot plant scale production of milk chocolate. Sensory evaluation was carried out using quantitative descriptive analysis by 10 trained panelists. Result showed that replacing sucrose with different types of polyols did not significantly (p≥0.05) affects the cocoa taste, sweetness, milky taste, smoothness and melting behavior of the chocolates in the mouth. Consumer test from 100 respondents indicated that chocolate with isomalt and maltitol were equally preferred while chocolate with erythritol was less preferred mainly due to the cooling sensation detected in this sample. The processing parameters used in this study were able to produce sucrose free chocolates with comparable particle size distribution to chocolate with sucrose (measured by Malvern particle size analyser, UK). Hardness analyses measured at 24°C using an SMS Texture Analyzer (TA.XT plus, UK) showed that freshly made milk chocolate with maltitol and isomalt were percieved to be significantly (p≤0.05) harder to break compared to chocolate with erythritol and sucrose. Chocolate containing isomalt and maltitol displayed a quite similar rheological behavior (by HADV 11+ Brookfield viscometer, UK) and chocolate with erythritol showed a closer rheological property to that of sucrose. Color (by Hunterlab MiniScan XE Plus, USA) and surface gloss (by Sheen Tri-microgloss, UK) analyses showed that the physical appearance of chocolates with polyols were fairly stable when stored at chill temperature (16±1°C).
Ngoc Minh Quynh Pham
Lecturer and researcher at Nha Trang University, Vietnam
Title: Effect of extraction solvents on saponin content and antioxidant capacity of Tuckeroo (Cupaniopsis anacardioides) fruit.
Time : 13:40-14:10

Biography:
Ngoc Minh Quynh Pham has over 10 years experience in research and teaching in the Food related subjects. Ms Pham is a lecturer and researcher at Nha Trang University, Vietnam. Presently, she is undertaking her PhD program at the University of Newcastle, Australia on a project entitled: “Extraction, isolation and identification of bioactive compounds of Tuckeroo fruit as novel therapeutic agents for prevention of pancreatic cancer”. She is a member of the Australian Pancreatic Cancer Research group and has presented her reseerach studies in various international conferences.
Abstract:
The Tuckeroo fruit is native to Australia and was found as a rich source of saponins, which are linked with various health benefits. As solvents play an important role in the extraction process due to their various polarities, this study aimed to determine the effect of five solvents including water, absolute ethanol, 50% ethanol, absolute methanol and 50% methanol on the total saponin content (SC) and antioxidant capacity of the Tuckeroo fruit. Freeze-dried fruit samples were prepared from the ripen fruit, and were extracted in each solvent at a ratio of 100:1 mL/g, using an ultrasonic bath set at 40 ï‚°C, 150 W for 30 minutes. SC and antioxidant capacity were spectrophotometrically measured and results were expressed as Aesin and trolox equivalent per gram of sample (AE/g and TE/g, respectively). Results showed that absolute methanol gave the highest yield of SC (1019 mg AE/g DW), followed 50% ethanol (1009), 50% methanol (802), absolute ethanol (672) and water (377) (mg AE /g DW). Results from the three antioxidant assays (ABTS, CUPRAC and FRAP) also revealed that absolute methanol gave the high antioxidant capacity, although the values were lower than those of 50% methanol. The findings of the current study further confirm that solvent has a significant impact on extraction yields of bioactive compounds. To obtain the extract enriched with saponins and potent antioxidant capacity from Tuckeroo fruit for further utilisation in the food and pharmaceutical industries, absolute methanol is recommended as the most effective extraction solvent.
Wiphada Mitbumrung
Institute of Nutrition, Mahidol University, Thailand.
Title: Encapsulation Efficiency and Stability of Vitamin D3 Enriched Emulsions Using Microfibrillated Cellulose as an Emulsifier
Time : 14:10-14:40

Biography:
Wiphada Mitbumrung graduated bachelor’s degree from Department of Biotechnology, Faculty of Science, Mahidol University. At present, she is a master’s degree student in food science for nutrition program at Institute of Nutrition, Mahidol University. Research interest is an application of natural hydrocolloid and Application of emulsion for nutrient and non-nutrient delivery system.
Abstract:
Microfibrillated cellulose (MFC) was an expanded cellulose prepared by hot alkali pretreatment and mechanical force. Normally, MFC was extracted from agricultural sources so, this study used mangosteen rind for MFC production because it was a by-product from the food industry.MFC could be used as a natural emulsifier for encapsulation of fat-soluble vitamin in emulsion system. The aim of this study was to investigate the effect of MFC concentration on emulsion properties and stability. Oil-in-water (O/W)emulsion; oil phase (0.01% vitamin D3, 9.99% soybean oil), aqueous phase (MFC, 10mM phosphate buffer pH7) was performed by using different MFC concentration (0.3, 0.5, 0.7% w/w) using a 2-stage high pressure homogenizer. Encapsulation efficiency, oil droplet size, color, ζ-potential, microstructure, and creaming stability of the emulsion samples were measured. The result found that MFC concentration affected to emulsion properties and stability. The emulsion containing 0.3, 0.5 and 0.7% MFC expressed encapsulation efficiency 87, 93 and 96%, respectively. All emulsions exhibited mean droplet diameter in range 10 to 100 µm. The increasing of MFC concentration exhibited larger oil droplet, provided darker color and increased magnitude of ζ-potential. SEM micrograph showed MFC formed physical barrier around oil droplet which provided stability of emulsion droplets against coalescence. However, it was found phase separation at concentration 0.3%MFC but did not cream at concentration 0.5 and 0.7%MFC throughout 90 days.
Ayaulym Nurgozhina
Junior researcher at National Laboratory Astana
Title: Food Frequency Questionnaire and FETA software to assess the dietary intake among Patients with Metabolic Syndrome
Time : 14:40-15:10

Biography:
Ayaulym Nurgozhina has completed Master of Science in Biomedical and Molecular Sciences Research at King’s College London and Bachelor of Science in Biological Sciences at Nazarbayev University. She is the junior researcher at National Laboratory Astana, established under the aegis of Nazarbayev University and Kazakstani government.
Abstract:
Patients from 25 to 75 years old with and without metabolic syndrome answered food frequency questionnaire. Total number of participants was 84. Questions covered all food preferences, daily intake number of fruits, beverages, milk products, etc. More than 120 food products are listed in the survey. The aim of the study was to identify intakes of different Vitamins in two groups by age. After questionnaire FETA tool was used to analyze all answers (http://www.srl.cam.ac.uk/epic/epicffq/). It is a tool to calculate nutrient and food group data from food frequency questionnaires. This research offered to see differences in more than 20 Vitamins in all age groups and patients with metabolic syndrome and healthy ones. The results showed differences in Vitamins by groups and gender. Patients with metabolic syndrome consume food almost twice more than healthy patients. Therefore, indicators of vitamins were much higher than normal index. For example, Iron’s Recommended Dietary Allowance is 8 mg however male patients with metabolic syndrome aged 60-75 have 23 mg per day as an average value. Recommended Dietary Allowance of each vitamin and mineral was compared with the values from the food frequency questionnaire. It was very important to find which product is responsible for the appearance of the vitamin index in the FETA software results. Moreover, it was suggested to add some more products which are common for Central Asian population such as horse meat, mare’s milk, etc. The results allowed concluding that a high accuracy of the FFQ FETA was achieved. This tool for converting food frequency questionnaire data into nutrient and food group values can be used widely for different study populations.
Kozhakhmetov Samat
Nazarbayev University, Kazakhstan
Title: Metabiotic based on Saumal (mare's milk)
Time : 15:10-15:40

Biography:
Samat Kozhakhmetov has completed her PhD at the age of 25 years from S.Seifullin Kazakh Agro Technical University and taken Degree of C.M.Sc at National Biotechnological Centre. He is a senior researcher of Human Microbiome Lab of National Laboratory Astana Nazarbayev University. She has published more than 80 papers in Kazakhstan journals, 10 in reputed Journals.
Abstract:
During performance of scientific project on order for government account metabolic compound pill having beside useful properties proven in befor clinical tests, was developed. During additional researches pill formula ont the base of natural mare's milk saumal was advanced. Offered product possesses expressed DNA by protective activities, possesses ability to increase adoptational properties of the organism, eliminates infringements of intestinal microbic bias, improves digestion, vegetable fibres and prebiotic improve motor functions of the intestines.
Results showed that probiotic strain Lactobacillus rhamnosus with DNA – protective action, at multiple application, does not cause infringements a functional condition of the main units and the systems of the organism, nor possesses toxic action.
Conducted before clinical researches of first-aid efficiency of strain Lactobacillus rhamnosus on the model of experimental toxic poisoning tetrachlormethan showed that probitoc Lactobacillus rhamnosus at reception in sufficient concentration is capable to lower disturbing influence of toxic гепатотропных substances to intestines microflora.
Studying of DNA-protective activity of the substance on the basis of living probiotical cells Lactobacillus rhamnosus in vivo, testified that daily reception of the substance “Probiotic withDNA-protective activity” during 7 days after creation of the model of sharp toxic poisoning tetrachlormethan, causes a first-aid efficiency.
New product produced from vegetable, animal and mineral raw materials of natural origin having pharmacological activity. It takes place between medical products and foodstuffs, and is ideally combined with rational therapy.
Almagul Kushugulova
Nazarbayev University, Astana, Kazakhstan
Title: The beneficial properties of a new synbiotic yoghurt “NAR†(ÐӘР)
Time : 16:00-16:30

Biography:
Almagul Kushugulova has completed her PhD at the age of 25 years from Astana Medical State University and taken Degree of D.M.Sc at National Biotechnological Centre. She is a head of Human Microbiome Lab of National laboratory Astana Nazarbayev University. She has published more than 100 papers in Kazakhstan journals, 15 in reputed Journals and has been serving as an editorial board member of Journal of Clinical Medicine of Kazakhstan.
Abstract:
Recent studies have shown that probiotic yoghurt consumption may have a positive role in the regulation of body weight as well as act to prevent cardiovascular diseases. Dairy products are a source of high quality protein, and due to the functional properties of live bacteria, yoghurt have unique properties. Objective is to test the efficacy of new a synbiotic formulation in adults with metabolic syndrome.
This randomized double-blind placebo controlled trial was conducted among 180 participants aged 25 to 75 years. Subjects with and without metabolic syndrome were randomly assigned to receive synbiotic or placebo daily for three months.
134 of 180 participants completed the study. Compared with the placebo group, the synbiotic group had significantly decreased total cholesterol.
Our study is the first large clinical trial of probiotics in Kazakhstan and the first study on the new synbiotic yoghurt NAR. The uniqueness of this product lies in the fact that it consists of probiotic component strains isolated from a traditional Kazakh koumiss product. Primarily, the product is aimed at people with digestive problems. Therefore, it is rich in easily digestible trace elements and amino acids, essential for the gut microbiota to flourish. We adhered to best practices for clinical trials to ensure that both investigators and patients were blinded to the study and that the data analysis was carried out by independent statisticians.
The present findings suggest positive influence of synbiotic on controlling excess weight and treating some metabolic disorders in adults.
Nilva Kazue Sakomura
Universidade Estadual Paulista “Júlio de Mesquita Filhoâ€, Brazil
Title: Ideal in-feed amino acid ratio for laying hens
Time : 16:30-17:00

Biography:
Nilva Kazue Sakomura has completed her PhD at Universidade Federal de Viçosa and postdoctoral studies at University of Arkansas (USA) and University of KwaZulu-Natal (RSA). She acts as professor at the Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCAV/UNESP and coordinates the Laboratory of Avian Sciences at same university. She participated in the elaboration of the last edition of Brazilian Tables for Poultry and Swine. Her research area is mainly foccused on modeling amino acid requirements of broilers, laying hens and broiler breeders.
Abstract:
The research was conducted to re-evaluate the assumptions of ideal ratios (IAAR) of the essential amino acids for Hy-line W-36 laying hens, using the amino acid deletion and nitrogen balance methods. The nitrogen balance trial was performed from 28 to 32 weeks of age, 12 treatments with 8 replicates and 1 bird per cage were used. A balanced diet (BD) was formulated to meet the IAAR and the requirement of other nutrients. The limiting diets were formulated diluting BD with corn starch and refilled with synthetic amino acids (AA) and other feed ingredients, except for the AA studied. The trial lasted 25 d, being the first 5 d for adaptation and the other 20 d for total collection of excreta and eggs. Diets, excreta and eggs samples were analyzed for nitrogen content to quantify nitrogen intake and excretion. From these data, nitrogen retention (NR) was calculated. The estimated requirement of each AA (AAR) was calculated as AAR= 100 x (1-NRi/NRcontrol) where NRi is the NR associated with each limiting diet calculated and NRcontrol is the NR associated with the balanced control diet. The estimated optimum ratio between each AA and Lys [(AA/Lys) * 100] and the optimum level of each AA (values in parentheses) were: Lys = 100 (0.673), Met+Cys = 82 (0.552), Thr = 69 (0.464), Trp = 21 (0.141), Arg = 104 (0.700), Val = 91 (0.612), Ile = 78 (0.525), Leu = 121 (0.814), Phe+Tyr = 119 (0.801), Gly+Ser = 77 (0.518) and His = 29 (0.195).
Yongmin Xiong
Director of the Endemic Institute of Xi'an Jiaotong University, China
Title: The Study on the Relationship between Selenium and Kashin-Beck Disease

Biography:
YongMin Xiong holds the positions of director of the Endemic Institute of Xi'an Jiaotong University, deputy director of the Key Laboratory of Trace Element and Endemic Diseases of National Health and Family Planning Commission, China. Her qualifications include a Bachelor of Medicine, PhD of Medicine. She is currently the Committee member of Society of Endemic Diseases of Chinese Medical Association and chairman of Trace Elements Branch in Shaanxi Province Medical Association. She has a particular intrest in the environment and gene interactions in the molecular mechanism of endemic osteoarthritis diseases. She has published more than 30 articles in reputed journals.
Abstract:
Background: Kashin-Beck disease (KBD) is an endemic, disabling and deforming osteoarthropathy, and mainly affects children or teenagers in growth and development period. The mainly pathological changes in KBD are degeneration and necrosis in joint cartilage and epiphyseal plate cartilage. The disease has been found over 160 years, but, its etiology remains unclear. Epidemiological investigation of environmental risks has shown that selenium deficiency may contribute to the etiopathogenesis of KBD and Se supplementation could significantly decrease the incidence of KBD. Thus, it is considered that Se deficiency is a main environmental factor of KBD, however, the exact molecular mechanism for KBD treatment with Se is still obscure.
Objective: Screening of KBD susceptibility genes and related functional experiments were conducted in order to illuminate molecular mechanism of selenoprotein on cartilage cell oxidative stress, inflammation and apoptosis signaling pathway, and further search new molecular targets for the early diagnosis, warning and treatment of KBD.
Methods: Selenoprotein gene transcription level, protein expression level and enzymatic activity in KBD and normal blood and cartilage samples were detected by using qRT-PCR, ELISA and Western blot. Selenoprotein single nucleotide polymorphisms (SNPs) were detected by using PCR-RFLP and ARMS-PCR. The protein expression levels of inflammation and oxidative stress signaling molecules in whole blood and chondrocytes were detected by western blot. The chondrocyte oxidative damage model was established using hydrogen peroxide tert butyl alcohol (tBHP), and the oxidative damage effects on apoptosis and oxidative stress, inflammation signaling pathways in chondrocyte were observed in the model to explore protective mechanism of selenium.
Results:1. The mRNA expression levels of 9 selenoprotein genes in blood and 4 in cartilage were detected, among which mRNA expression of GPx1, GPx4, SEPP, TrxR and DIO2 in whole blood decreased in the KBD group compared to controls, and mRNA expression of GPx1, GPx4 and DIO2 in cartilage decreased in the KBD group compared to controls. 2. In this experiment, 11 SNPs from 7 important selenoprotein genes were screened, the results showed that GPx1Pro198Leu, GPx4Haplotype(rs713041, rs4807542), SEPS1(rs28665122, rs34713741), SEP15 rs5859 displayed significant differences in genotypic and allelic frequency between the KBD and control groups, while TrxR2(rs5748469, rs1139793, rs5746841),SEPP1(rs7579) and DIO2 (rs225014) showed no significant differences. 3. The protein expression levels of inflammation and oxidative stress signal transduction pathways were observed between KBD patients and controls. The protein expression levels of PI3K/AKt, ERK, JNK, Nrf2-ARE, NFκB and AP1 signal molecule in KBD group were significantly higher than that in control group, while protein expression levels of ERK in KBD patients was decreased than that in control group, indicating that signaling pathways related to oxidative stress, inflammation, apoptosis were disordered in KBD patients. 4. In genotype subgroup analyses, GPx enzyme activity decreased in the variant genotype GPx1Pro198Leu, and PI3K/AKt signaling pathway were up-regulated in the variant genotype (AA) individual in SEPS1-105G>A (rs28665122). These suggested that selenoprotein polymorphism had important role in regulating GPx enzyme activity and PI3K/AKt signaling. 5. 300 μmol/L tBHP could induce apoptosis and suppress cell survival in human chondrocyte C28 cells, the up-regulation of protein expression levels of c-jun, p-c-jun, MEKK1, p-JNK, AP-1 and the down-regulation of Bcl-2 were observed. Pre-protection with Na2SeO3 (0.05 μg/mL, 0.1 μg/mL) could ameliorate the cell apoptosis, inhibit the ROS generation, and regulate the protein expression levels of the signaling molecular.
Fatemeh Mahmoodani
School of Chemical Sciences, University of Auckland, New Zealand
Title: Isolation and identification of vitamin D3 oxidation products in simulated whole milk powder by liquid chromatography mass spectrometry

Biography:
Fatemeh Mahmoodani is currently a final year PhD student at the School of Chemical Sciences, University of Auckland, New Zealand. Her current research focuses on vitamin D3 degradation in whole milk powder and identification of vitamin D3 isomerization and oxidation products. Her PhD project is collaboration with Fonterra Cooperative Group Ltd. and funded by the Primary Growth Partnership Program of New Zeeland.
Abstract:
In a successful fortification program, the stability of micronutrients added to the food is one of the most important factors. The added vitamin D3 is known to sometimes decline during storage of fortified milk powders, and oxidation through fatty acid lipoxidatoin is suspected as the likely cause. The main objective of this study is to find a method to extract and to identify vitamin D3 oxidation products (VDOPs) in stored whole milk powder. Identification of VDOPs in natural foods is a challenge due to the low amount of their contents. An extraction method using a liquid-liquid extraction (LLE) followed by a solid phase extraction (SPE) was optimized to extract VDOPs. The bottleneck of the VDOPs’ identification could not be overcome without mass spectra prediction tools. The fragmentation trees and MSn spectral trees provided by Mass Frontier software gave reliable methods to identify vitamin D3 unknown compounds such as VDOPs. Methods based on high mass accuracy MS2 and MSn analysis have been developed for the identification of vitamin D3 and its oxidation products. The multi stage mass spectrometry (MSn) spectra can help to propose plausible schemes for unknown compounds and their fragmentations. This study was focused on identifying the fragmentation rules for some VDOPs by incorporating MS data with in silico calculated MS fragmentation pathways. Diels-Alder derivatization was used to enhance the sensitivity and selectivity for mass spectrometry data collection. Finally, the confirmed PTAD derivatised target compounds were separated and analyzed using ESI(+)-UHPLC-MS/MS in multiple reaction monitoring (MRM) mode in model samples.
Yongmin Xiong
Director of the Endemic Institute of Xi'an Jiaotong University, China
Title: The Study on GPX3 DNA Methylation for the Cartilage Damage Mechanism in Kashin-Beck Disease

Biography:
YongMin Xiong holds the positions of director of the Endemic Institute of Xi'an Jiaotong University, deputy director of the Key Laboratory of Trace Element and Endemic Diseases of National Health and Family Planning Commission, China. Her qualifications include a Bachelor of Medicine, PhD of Medicine. She is currently the Committee member of Society of Endemic Diseases of Chinese Medical Association and chairman of Trace Elements Branch in Shaanxi Province Medical Association. She has a particular intrest in the environment and gene interactions in the molecular mechanism of endemic osteoarthritis diseases. She has published more than 30 articles in reputed journals.
Abstract:
Kashin-Beck disease (KBD) is an endemic and disabling osteoarthropathy. Epidemiological investigations have shown that selenium deficiency may contribute to the etiopathogenesis of KBD, however, the mechanisms of Se deficient in cartilage damage of KBD are still not clear. Glutathione peroxidases3 (GPX3), a member of selenoproteins, is found to paly a crucial role in protecting against oxidative damage in human body. The aim of the study was to investigate the effects of GPX3 DNA methylation on cartilage damage. GPX3 methylation were detected in whole blood of KBD patients and chondrocytes by methylation-specific polymerase chain reaction. The GPX3 mRNA were measured in whole blood of KBD patients and chondrocyte by qRT-PCR. The expression of PI3K/Akt/c-fos signaling molecules in whole blood of KBD patients and chondrocytes using Western blotting. The results showed that the frequency of GPX3 methylation significantly was increased and GPX3 mRNA was decreased in KBD patients than that of controls, and protein expression of Gβγ, PI3Kp110, pAkt and c-fos in KBD group were up-regulated. In the cell experiments, the rates of cell apoptosis and GPX3 methylation were increased, and GPX3 mRNA was decreased and PI3K/Akt/c-fos expression were up-regulated in injured group using tert-Butyl hydroperoxide. However, The rates of apoptosis and GPX3 methylation were decreased, GPX3 mRNA was increased and PI3K/Akt/c-fos signaling pathway were down-regulated in three Se pre-protection groups, suggesting that aberrant DNA methylation of GPX3 might be one of cartilage damage mechanisms in KBD (This research were supported by National Natural Science Foundation in China No.81573104, 81773372).
Fathimah Sulistyowati Sigit
PhD Student at Leids Universitair Medisch Centrum
Title: Anaemia in Women in Reproductive Ages and Its Relation to Dietary Intake and Individual Factors: A Study in Rural Community in Pekalongan Regency, Indonesia

Biography:
Fathimah Sulistyowati Sigit is a Medical Doctor graduated from the University of Indonesia. She then continued her Master’s degree at Imperial College London. Currently, she is pursuing her PhD at Leiden University Medical Center. Prior to her PhD, she worked as a General Practitioner (GP) in a rural community of Pekalongan, Central Java, Indonesia.
Abstract:
Dean A. Sewell
Heriot-Watt University, School of EGIS, Edinburgh, U.K.
Title: Towards reducing allergy prevalence from an early age

Biography:
Dean Sewell is an experienced researcher and teacher with experience in both academia and industry. He is a Registered Nutritionist, and a former Honorary External Affairs Officer of The Nutrition Society (UK). Collaboration has resulted in a network of contacts locally, nationally and internationally.
Abstract:
IgE-mediated allergy such as eczema, food allergy, hayfever and asthma may affect 50% of the paediatric population. Diet may have a role in the primary prevention of allergy as the pre-natal period and the first few months of life are a critical period when there can be modulation of growth and development of the respiratory, immune and gastrointestinal systems. It has been hypothesised that maternal diet during pregnancy modulates the development of allergy and asthma by influencing foetal airway and/or immune development. Reducing or delaying allergic disease onset in early life could have a significant public health impact.
Our programme of work to date includes an extensive systematic review and meta-analysis examining the strength of the scientific evidence for associations between dietary intake of foods and nutrients by pregnant women and children and the risk of children developing allergy and asthma. Results found a potentially protective role for candidates including the Mediterranean diet (MD), and we hypothesise that adherence to a MD might represent an effective primary prevention strategy. Although observational studies have reported potentially beneficial associations with a MD, it remains to be seen whether an intervention to promote the MD reduces the likelihood of childhood allergy and asthma. This needs to be investigated through formal experimental studies, as there are currently no randomised controlled trials (RCT’s) testing the hypothesis that enhancing MD adherence in the mother will decrease the risk of allergic disease in children. We have engaged in a programme of work to design an adequately powered RCT to investigate the potential protective effects of the MD on the risk of developing allergy and asthma. Our pilot RCT investigated maternal recruitment and retention to a MD intervention and we have published the protocol and results, and shortly, a follow-up of the infants born to women who participated in the pilot RCT. Infant follow-up included allergic outcomes at 6 months determined through birth and environment data, and at 12 months of age determined through clinical history, allergen skin prick testing (SPT) and clinical examination.
We will soon begin a well-designed and adequately powered RCT to investigate the potential protective effects of the MD on the risk of developing allergy and asthma, following a framework for developing and evaluating complex interventions.
Dr. Xiao Su
Associate Professor, Victoria University, Australia.
Title: Krill oil extract inhibits proliferation of human colorectal cancer cells through mitochondrial pathway

Biography:
Dr Xiao Su is an Associate Professor in the Victoria University, Australia. She completed her PhD study and postdoctoral training at the University of Tasmania, Hobart, Australia in 1995. She then took an academic position at the Victoria University, Australia and has worked there since 1996. Dr Su’s research focuses on the therapeutic role of natural products in chronic disease including obesity, diabetes and cancer. She has received several national and international research grants, and has published widely in the quality scientific journals. She has been invited to present at the international conference and is a reviewer of several international journals.
Abstract:
Colorectal cancer (CRC) is one of the major causes of cancer death worldwide. The currently available chemotherapy is associated with numerous side-effects. Therefore, the effective nutraceutical agents with low or no side-effects are desirable. Krill oil (KO) is a rich source of long-chain omega-3 polyunsaturated fatty acids, mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Our preliminary data showed that the free fatty acid extract (FFAE) from the KO suppresses cell growth and induces apoptosis of human CRC cells. The aim of this study was to investigate the effect of FFAE from KO and potential mechanism underlying it’s anti-cancer effect on human CRC cells. DLD-1, HT-29 and LIM-2405 cell lines were treated with EPA (50 - 200 µM), DHA (50 - 300 µM) and FFAE of krill oil (0.03 - 0.24 µL/100µL) for 24h and 48h to evaluate their effect on cell proliferation using a water-soluble tetrazolium-1 (WST-1) assay kit. The effect of FFAE from KO (0.12 µL/100µL), EPA (200 µM) and DHA (250 µM) on mitochondrial membrane potential was determined using a JC10 mitochondrial assay kit, and the effect on reactive oxygen species (ROS) was determined using a MitoSOX™ Red M36008 kit. The FFAE of KO (0.06 - 0.24 µL), EPA (200 µM) and DHA (200 - 300 µM) significantly inhibited the cell proliferation in all three CRC cell lines. Moreover, they have reduced the formation of ROS. Treatment with the FFAE of KO also resulted in a significant increase in the mitochondrial membrane potential. However, no such change was observed after EPA and DHA treatments. These results suggest that the anti-proliferative effect of FFAE from KO may be associated with the intrinsic (mitochondrial) pathway although other active components are yet to be determined. The supplementation with krill oil may provide a novel and safe therapy for CRC treatment.
Xiaofang Jia
National Institute for Nutrition and Health, China
Title: Dietary intake, food sources and potential determinants of vitamin C in Chinese adults

Biography:
Xiaofang Jia has completed her PhD in the Graduate School of Medicine, Nagoya University, Japan. Currently, she is an associate professor in National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, and interested in the field of Nutrional Epidemiology. She has published more than 10 papers in reputed journals.
Abstract:
Vitamin C is essential for human health. However, dietary vitamin C intake in Chinese population have changed with nutritional transition in recent decades and needs to be estimated. Present study used data of “China Nutritional Transition Cohort Study” in 2015 and selected those aged 18-65 years with completed data of socio-demographic characteristics, anthropometry and dietary measurements as subjects. We analyzed daily dietary vitamin C intake and food sources across demographic factors, evaluated the prevalence of vitamin C deficiency using the estimated average requirement cut-off point, and explored underlying influencing factors. Mean and median levels of dietary vitamin C intake in adults was 78.1 and 65.4 mg/day, respectively. Prevalence of vitamin C deficiency in study population and proportion of subjects with lower probability of vitamin C inadequacy were 65.1% and 25.9%, respectively. Both the distribution of vitamin C intake and the prevalence of vitamin C deficiency differed by gender, educational level, residence area, geographic location, smoking, alcohol intake and vegetable consumption frequency. Moreover, gender, educational level, residence area, geographic location and vegetable consumption were found to be independent determinants for vitamin C deficiency. Top four food sources of vitamin C in adults were light vegetables, dark vegetables, fruits and tubers, totally contributing 97.3% to total vitamin C. In conclusion, dietary vitamin C deficiency is severe in Chinese adults. Vitamin C intake on a recommended basis in Chinese population is required, especially women and population living in town and rural regions.
LI LI
National Institute for Nutrition and Health, China.
Title: Thiamin, Riboflavin and Niacin intakes in Chinese adults in 15 provinces
Biography:
LI Li had graduated from the London School of Hygiene & Tropical Medicine in 2005 with a master's degree in Medical Parasitology. From 1996 to 2012, She mainly engaged in infectious diseases control, scientific research and teaching in province level. Since 2013, she joined in China CDC, and mainly involved in food safety, foodborne diseases control and nutrition epidemiology in National Institute of Nutrition and Health, China CDC. She has published more than 8 papers in reputed journals.
Abstract:
B vitamins have been neglected in China. Most people who lack it belong to subclinical conditions. Well understanding the prevalence of B vitamins will provide guidance for nutritional policies and interventions in China. The data were used from a longitudinal survey in 2015, China health and nutrition survey (CHNS) among 18-64 year old. Dietary data of subjects on three consecutive days were recorded. The EAR cut-point method (EAR cut-point) was used to assess the prevalence of the adequacy intake of three B vitamins, thiamin, riboflavin and niacin by sex, age, areas of residence, south and north parts of China, education, and income. For male, the prevalence of inadequate intake of vitamin B1,B2 and niacin was 77.39%,85.47% and 25.60% respectively. For female, the prevalence of inadequate intake of vitamin B1, B2 and niacin was 75.84%,81.71% and 26.49%, respectively. Vitamin B1 and B2 are seriously deficient in both sex in 18-64 year old adults. Better income or better education levels of both sex have higher intake of vitamin B2 and niacin than those with lower incomes or education. The intake of vitamin B1, B2 and niacin is inadequate among 18-64 years old in China. Improved nutritional status in China is needed by well-tailored strategies and policies.
Ping Zhang
Jinan University, China
Title: Alcohol Exposure Suppresses Neural Crest Cells Generation and Differentiation during Early Chick Embryo

Biography:
Ping Zhang, a graduate student in Jinan University, China, currently working in the Key Laboratory for Regenerative Medicine of the Ministry of Education and Division of Histology & Embryology, under the guidance of professor Xuesong Yang. Maintaining a high degree of enthusiasm and professional research attitude, she is been devoted into the program “Alcohol exposure induces chick craniofacial bone defects by negatively affecting cranial neural crest development” and has published the research results on Toxicology Letters.
Abstract:
It is now known that excess alcohol consumption during pregnancy can cause fetal alcohol syndrome (FAS) in which several characteristic craniofacial abnormalities are often visible. However, the molecular mechanisms of how excess ethanol exposure affecting cranial neural crest cells (CNCCs), the progenitor cells of the cranial skeleton, is still not clear. In the study, we investigated the effects of ethanol exposure on CNCCs migration both in early chick embryo and in vitro explant culture. First of all, we demonstrated that ethanol treatment caused alizarin red-stained craniofacial developmental defects including parietal defect. Second, Immunofluorescent staining with neural crest special markers indicated that CNCCs generation was inhibited by ethanol exposure. And, double immunofluorescent stainings (Ap-2α/PHIS3, HNK1/BrdU and AP-2α/c-caspase3) revealed that ethanol exposure inhibited CNCCs proliferation and increased apoptosis. In addition, it inhibited NCCs production by repressing the expression level of key transcription factors which regulate neural crest development by altering expression of Epithelial-mesenchymal transition (EMT)-related adhesion molecules in the developing neural crests. In sum, we have provided experimental evidence that excess ethanol exposure during embryogenesis disrupts CNCCs survival, EMT and migration, which in turn causes defective cranial bone development.
Biography:
Hafiz Arbab Sakandar is a graduate in School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People’s Republic of China.
Abstract:
The present study was undertaken to isolate and characterize gliadin hydrolyzing microbiota from locally fermented sourdoughs to evaluate their potential as a candidate for probiotics. Sixty autochthonous sourdough samples were collected from different areas. Eighteen bacterial and three yeast isolates were screened for the presence of gluten-degrading ability. These isolates were subcultured on different media for presumptive preselection by biochemical assays. Five bacterial isolates were LAB and two were bacillus among 18 bacterial isolates which were further characterized based on autoaggregation, ß-galactosidase activity, antibiotic susceptibility, inhibition against pathogens, bile salts tolerance, pH tolerance, surface hydrophobicity and cholesterol assimilation. In this study Lactobacillus plantrum ATCC 14917 was used as control strain to compare the probiotic potential of isolated strains. 16S and 28S rRNA sequencing revealed the presence of four Enterococcus faecalis strains along with Enterococcus mundtii, Bacillus cereus and Bacillus megaterium strains. Yeast strains were identified as Wicherhamomyces anomalus. Among all these strains E. mundtii QAUSD01 and W. anomalus QAUWA03 had the ability to tolerate low pH, bile salt properties and hydrophobicity compared to other gluten-degrading strains. These two strains exhibited the potential to be used as a probiotic for sourdough fermentation.
Souheir Alia
Clinical dietitian, Rashid Hospital, UAE
Title: Assessment of Nutrition Knowledge, and Dietary Behavior of Post Bariatric Surgery Patients in Rashid Hospital Outpatient Clinic in Dubai, UAE.

Biography:
Souheir Alia, has her expertise in health education and promotion in both community setting as well as hospital setting, and works as a clinical dietitian in Rashid Hospital, DHA, in the city if Dubai, in UAE, and is in the process of finishing the requirement to finish her masters’ degree in food science, and her thesis being research based focusing on patients post bariatric surgery dietary protocol.
Abstract:
Obesity is considered to be a disease, which stands alone by itself, and it is accompanied by much co-morbidity, and that is why many means to treat obesity comes into account and one of the most prevalent ways in UAE is by bariatric surgery. Despite the vast research assessing nutrition knowledge of patients of several health conditions, none of the papers assessed the nutrition knowledge of patients’ post-bariatric surgeries, although this category of patients is very susceptible to malnutrition post-surgery. My aim in this study was to assess the general nutrition knowledge and the knowledge specific to the dietary protocol post-surgery, the medical and nutritional complications, and their awareness and understanding of dumping syndrome specifically, the clarity of information conveyed by the dietitians and its effect on their levels of compliance of the post-bariatric surgery dietary protocol and the follow up appointments with the dietitians, and finally a small part gave a glimpse of their quality of life post-surgery.
The methods used to analyze the questionnaire were with the help of SPSS version 23.0. Descriptive statistics such as frequencies, proportions, means, and standard deviations were used. Statistical tests such as Chi-Square test of independence, and Pearson’s correlation were used to test correlation. To test significant differences between values of quantitative variable were used using the statistical test ANOVA or its equivalent non-parametric test named Kruskal-Wallis. Normality was tested using the Shapiro-Wilk test, while Levene’s test was used to test the equality of variance.
The results of this study showed a fairly good general nutrition knowledge of both groups, the questions assessing nutrition knowledge of the dietary protocol post-surgery were added up and given a score out of 14, in which it showed that only 19.4% of participants had very good knowledge, 66.2% had average knowledge, and 14.4% had poor knowledge. In addition, most patients didn’t know what dumping syndrome is, and of those who knew what it is 66.6% of them knew the food that promote its occurrence, and almost half of the participants who answered yes knew the symptoms of it. On the other hand, 79.5% of the patients followed up with a dietitian, and only 30.1% showed compliance to the dietitian’s instructions, which was strongly related to patients finding the information conveyed vague and unclear as 71.2% considered it as aforementioned. However, the most experienced symptom post-bariatric surgeries were nausea, followed by dizziness, dehydration, and finally vomiting. As for the overall quality of life of participants the highest percentage 45.8% was given to participants who never felt agitated, fatigued and/or regretted their decision of getting operated, and as much as 83.1% found their daily activities to be more enjoyable. In conclusion, patients who undergo bariatric surgeries are a great area of improvement now that we can spot some gaps in the health care provided.
Mona Ahmed J. Alzahrani
School of Chemical Sciences – Food Science,University of Auckland, New Zealand
Title: Bioactive Proteins and Enzymatic Hydrolysates from Nitzschia laevis and their in vitro Antioxidative & Antihypertensive Activities

Biography:
Mona Ahmed J. Alzahrani is a PhD student at the School of Chemical Sciences – Food Science. Mona completed her master’s degree in Engineering, specialized in chemical and materials engineering from the University of Auckland (2011). Mona also studied a PGDip in Science – Food Science at the University of Auckland. In 2007, Mona obtained her Bachelor’s degree from Umm Al-Qura University in Saudi Arabia specialized in Nutrition and Food sciences. Professionally, Mona has managed a number of municipal health-related projects in Saudi Arabia. Mona’s research interests lies in novel food processing technologies, mainly as an aid for extraction process of bioactive compounds from microalgae as a sustainable approach compared to conventional sources.
Abstract:
Nitzschia laevis is used to extract highly pure eicosapentaenoic acid (EPA) for commercial use in food and pharmaceutical applications, and the biowaste is used for animal feed. This research focuses on the aqueous production of bioactive proteins and hydrolysates from the by-product of EPA production from N. laevis. A comparison of antioxidant and antihypertensive activities was established between the extracts of N. laevis and other well-known microalgae that are used as food ingredients/supplements. In this study: 1) Proteins were extracted from N. laevis, 2) Hydrolysates were produced from proteins extracts using Alcalase®CLEA™, Flavourzyme® and Trypsin, 3) Proteins and hydrolysates were purified and partially characterized, 4) in vitro bioactivities were screened using chemical and enzymatic assays. The hydrolysis process enhanced the antioxidant and antihypertension activities in general, especially those obtained using Alcalase®CLEA™. Nitzschia showed the highest total phenolic content and reducing capacity (2.4±0.02mg GAE/100g) after 90 minutes of hydrolysis with Alcalase®CLEA™. Moreover, hydrolysates at 120 minutes showed the highest ABTS scavenging activity (66.77±0.003%), but DPPH was low (29.599±0.024%). A correlation study was established between DPPH and ABTS scavenging activity with total phenolic contents, trypsin hydrolysates showed the highest positive correlation. The antihypertensive activity was significantly enhanced after hydrolysis; Alcalase®CLEA™ hydrolysates of N. laevis showed higher ACE inhibition activity after 30 minutes (64.81% ACE inhibition) and Flavourzyme® hydrolysates also showed high activity after 60 minutes (60.81% ACE inhibition) compared to controls. The interactions between proteins and hydrolysates with other residues are likely to contribute to the measured antioxidant and antihypertension activities.
Nisreen Rajeh
KAU, School of Medicine, KSA, Saudi Arabia
Title: Antioxidant effect of vitamin E and 5-aminosalicylic acid on acrylamide induced kidney injury in rats
Biography:
Dr.Nisreen Rajeh has completed her PhD at the age of 40 years from SurreyUniversity (UK) and postdoctoral studies from KAU University School of Medicine. she has published more than 10 papers in reputed journals.
Abstract:
Objectives:
To explore renal toxicity caused by sub-acute exposure of acrylamide, which is known food toxicant , and to study the protective effect of 5-Aminosalicylic acid (5-ASA) and Vitamin E (vit-E)on Acrylamide (ACR) induced renal toxicity.
Methods:
This study was conducted at King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, between August and November 2015. A total of 49 adult Wistar rats (250 Â} 20g) aged 60 days were kept in a controlled environment and used in the present study. The rats were divided into 7 groups (control, ACR alone, ACR+5-ASA, ACR+vit-E, ACR+ASA+vit-E, vit-E alone, and ASA alone). After 5 days of ACR oral gavage treatment, the rats were observed for 24 hours then killed. Histopathology for the kidney and lactate dehydrogenase assay were carried out.
Results:
Acrylamide produced significant pathological changes in the kidney with acute tubular necrosis in the distal tubules that could be reversed by concomitant injection of rat with 5-ASA. Together with vitamin E, 5-ASA, showed maximum renal protection. No statistically significant difference was observed in either body weights or lactate dehydrogenase activity of ACR treated rats.
Conclusion:
Acrylamide exposure leads to adverse clinical pathologies of renal tubules, which were reversed by a concomitant treatment with 5-ASA and vitamin-E .
Gülşen Delikanlı
Department of Nutrition and Dietetics, Trabzon, Turkey
Title: The Effect of Education and Socioeconomic Level of Women on Infant Nutrition Knowledge Level and Practices
Biography:
GülÅŸen Delikanlı is a research assistant in Department of Nutrition and Dietetics, Trabzon, Turkey.
Abstract:
Statement of the Problem: It’s been found that many factors such as education and socioeconomic level are effective on breastfeeding and infant feeding practices in studies conducted in developed and developing countries. Due to untrained and unconscious eating behaviors of the mothers, the breastfeeding activity is not ensured sufficiently. The level of development of the countries is determined by the health level of the children in that country and compared with the child mortality rate. The purpose of this study is to determine the knowledge levels and behaviors for breastfeeding and complementary nutrition of mothers 0-24 month postpartum. Method:The age, occupation and educational level of 250 women, the duration and frequency of breastfeeding of the infants were questioned.The level of knowledge and applications of maternal breastfeeding and infant feeding have been determined. Results: Mothers with low levels of education and socioeconomic status have worse knowledge of infant feeding. There was a statistically significant difference with breastfeeding duration and thinking to continue breastfeeding with the increase in the educational level. There was no statistical significance in the level of knowledge and practice according to education level. Conclusion: The knowledge level of women about breastfeeding and infant feeding varies according to the level of education and the place they live. Increasing the education level of women, developing courses and skills is important for healthy infants and society. All employees involved in basic health services should be educated about the importance of breast milk and breastfeeding.
Nabil Bosco
Nestlé Research Center, Singapore.
Title: Frailty Does Not Impair Influenza Vaccine-Induced Immune Responses in the Elderly

Biography:
Nabil Bosco currently works at the Nestlé Research Center, Nestlé S.A.. Nabil does research in Human Biology, Cell Biology and Immunology.
Abstract:
BACKGROUND
Elderly adults over 65 years of age are recommended to receive seasonal influenza vaccination as they are at a higher risk of infection than the younger community. The elderly population is often stratified according to frailty status where frail individuals are more susceptible to adverse health outcomes than their non-frail counterparts, however, it is not known whether protective immunity induced by influenza vaccination is impaired in the frail elderly.
STUDY DESIGN
205 elderly subjects of Chinese ethnicity in Singapore (mean age 73.3 ± 5.3 years, 128 females and 77 males) were administered the recommended trivalent inactivated 2013-14 seasonal influenza vaccine (VaxigripÔ) containing A/H1N1, A/H3N2 and B strains. The elderly subjects were stratified into three groups according to Fried’s frailty criteria (59 frail, 85 pre-frail, 61 robust) and were also ranked by Rockwood’s frailty index (RFI) or nutritional status. Statistical associations were evaluated between frailty status, pre- and post-vaccination antibody titers in sera, modulation of leukocyte populations, cytokine responses and gene expression profiles of peripheral blood mononuclear cells.
RESULTS
Post-vaccination, 93.2% of the elderly were seroprotected against A/H1N1, 97.1% against A/H3N2 and 99.5% against the B strain. Seroprotection rates were similar in all three frailty groups (Anova, A/H1N1, p = 0.605; A/H3N2, p = 0.585, B, p = 0.585) and uncorrelated with RFI (Spearman, r = 0.023, p = 0.738). No statistically significant differences were observed in vaccine-induced modulation of leukocyte populations, cytokine responses and gene expression profiles of peripheral blood mononuclear cells.
CONCLUSION
The majority of the elderly subjects were seroprotected against seasonal influenza upon vaccination, and importantly, influenza vaccination-induced humoral immune responses and seroprotection were similar across the frailty strata, indicating that frail individuals may also benefit from influenza vaccination.
Joao Batista Kochenborger Fernandes
Specialized Fish Technicia, Aquaculture Center of UNESP, Brazil
Title: Deletion method to establishing the optimal essential amino acid ratio for juveniles of pacu (Piaractus mesopotamicus)

Biography:
Joao Batista Kochenborger Fernandes has completed his PhD at the age of 25 years from “Universidade Estadual Paulista Júlio de Mesquita Filho”, UNESP, Brazil and postdoctoral studies from “University of Arkansas, USA and University of Kwazulu-Natal”, UKZN, South Africa. Since 1990, he has been a Specialized Fish Technician at the Aquaculture Center of UNESP, Jaboticabal-SP, Brazil, developing activities with rural producers through consultancies, projects and technical assistance.
Abstract:
The aquaculture look for more and more an efficient production, less environmental impact and increased profitability. Knowing that the correct feeding and balanced diet promotes maximization of growth and health, reduce the production costs and water pollution, the present study aimed to estimate the essential amino acids (EAA) ratio for juveniles of pacu (Piaractus mesopotamicus) using the deletion method. For this, 635 pacus (6.23g) were distributed in thirty three tanks connected to a closed water recirculation system equipped with filter, water heater (27.8ºC) and aeration. The experiment lasted 113 days and consisted of 11 experimental diets with three replications each, distributed in a completely randomized design. The fishes were fed twice a day until apparent satiation. The semi-purified and extruded diets were formulated by the deletion technique, where the control diet presented all the EAA in a balanced way, while the experimental diets presented the deletion of 45% of the respective tested EAA. In this way it was possible to obtain 11 isonutrient diets varying only the tested EAA. The determination of the essential amino acids ratio was performed by the ratio between the nitrogen retention in fish body and the amount of the EAA deleted in the tested diet. Thus, assuming that each EAA is equally limiting, our results show the ideal dietary EAA profile relative to lysine was estimate to be: lysine 1.000, arginine 0.593, threonine 0.336, valine 0.282, leucine 0.396, isoleucine 0.220, phenylalanine 0.226, methionine 0.126, tryptophan 0.063.
Pedro M. Rodrigues
Universidade do Algarve, Portugal.
Title: Allergenicity modulation of farmed fish

Biography:
Pedro M. Rodrigues has a PhD in Chemistry from the Universidade Nova de Lisboa and is presently a Professor at University of Algarve, Portugal since 2000 and a member of the Center of Marine Science of the Algarve. He has been working in the use of proteomics in Aquaculture to access fish quality, welfare and allergies for the past 10 years and he has been involved in several research projects, two COST actions and published 34 papers in reputed journals, 3 book chapters and has also been serving as an editorial board member of repute.
Abstract:
Food allergies are a significant public health concern throughout the world. Fish is a food product increasingly consumed worldwide due to its high nutritional value and healthy meat. Unfortunately, like many food sources, fish can cause adverse immune-mediate reactions in some individuals. This allergic reaction to food, affects 1-3% of the population and about 4-6% of children. The main fish allergen is β-parvalbumin; a small and highly stable muscle protein. Fish parvalbumins are highly conserved proteins, which are binding bivalent ions, calcium or magnesium. In fish-allergic patients, specific IgE cross-react mostly with parvalbumins from different fish, especially when recognizing highly identical protein regions involved in the ion binding. Other fish allergens have been identified as well - such as enolases, aldolases or fish gelatin - but their importance has been only shown for a limited number of fish species. Currently, there is no cure available for fish allergies with the clinical management of fish allergy solely relying on a strict avoidance diet. Modulation of fish allergenicity towards the production of a low allergen farmed fish was firstly attempted by our group. This has been done with specifically designed fish diets, enriched in components and small changes introduced in the fish farming process that target the expression or inactivation of the main fish allergen; parvalbumin. Proteomics is the chosen technique to access fish allergens characterization and expression in muscle while IgE assays to confirm the lower allergenic potential of this fish are conducted in patients with history of fish allergies.
Selekane Ananias Motadi
University of Venda, South Africa
Title: Overweight and obesity among adults aged 18-45 years residing in and around Giyani town in Mopani District of Limpopo province, South Africa

Biography:
Mr. Selekane Ananias Motadi is a junior lecturer of Nutrition at the University of Venda. He received his junior degree BSc in Nutrition at the University of Venda. In 2010, He registered for Master Degree in Public Nutrition at the same university. He is a registered Nutritionist with Health Professional Council of South Africa. He was offered tenure in the Department of Nutrition. In addition to teaching, Mr. Motadi is a regular contributor to the micronutrient malnutrition particularly zinc and a Mamelodi sundowns United fan. He has registered for postgraduate diploma in health professional education at the University of Cape Town which he envisages completing in 2015. He has collaborated on manuscripts with Prof XG Mbhenyane, Dr. RL Mamabolo, Ms. HV Mbhatsani and Mr. NS Mabapa entitled “Prevalence of zinc deficiency among children aged 3-5 years in Vhembe district, Limpopo province, South Africa”. He currently resides in Limpopo Province, South Africa with his brother.
Abstract:
Objective: To describe the prevalence of overweight and obesity among adults aged 18-45 years residing in and around Giyani town.
Methods: This study included 100 participants recruited from a location in Greater Giyani Municipality of Mopani district, Limpopo Province, South Africa. This Municipality was purposively selected and convenience sampling was used to choose study participants. Body weight and height were measured using standard techniques. Waist circumference, systolic, diastolic, glucose, cholesterol and body fat levels were also assessed.
Results: The prevalence of overweight and obesity was 18.1% and 20.5%, respectively. Obesity was more prevalent in females (19%) than males (1.5%). The risks found in study participants included hypertension, diabetes, high total cholesterol, alcohol consumption and cigarette smoking. There was a significant difference in systolic blood pressure (p≤0.03) between males and females. Systolic blood pressure of about 22.9% of male participants compared to females (20%) was in the hypertensive range. However, only 9.2% of females had prehypertension of between 85-89 mmHg. Furthermore, 17.1% of males had hypertension values ≥90 mmHg as compared to 9.2% of their female counterparts.
Conclusion: Overweight and obesity were common in adults residing in urban areas. The results observed here call for intervention to combat the rising problem of overweight and obesity among adults in the form of nutrition education to ensure food diversification and reduction of food containing fats and hidden fats.
Keywords: overweight, obesity, body mass index, body fat, waist circumference, total cholesterol.
Su Bin Park
Gyeongsang National University, Korea
Title: Aruncus dioicus var. kamtschaticus Ameliorates Trimethyltin-induced Neurodegeneration in ICR mice

Biography:
Su Bin Park has been completing her master’s degree from Gyeongsang National University, division of Agriculture and Life science. She has published two domestic papers associated with functional foods
Abstract:
To estimate physiological effect of ethylacetate fraction of Aruncus dioicus var. kamtschaticus (EFAD) on cognitive dysfunction, in vivo and ex vivo test were conducted in neurodegenerative animal model. The animal model is established as injecting trimethyltin (TMT) (7.1 ðœ‡g/kg of body weight, intraperitoneal injection) in Institute of Cancer Research (ICR) mice. EFAD showed the excellent ameliorating effects on reduced spatial memory ability, short- and long- term memory deficits in the mice through in vivo tests such as the Y-maze, passive avoidance and Morris water maze test. After the behavioral tests, to ascertain the protective effects of EFAD on the cholinergic and antioxidant system, a series of biochemical indicators were measured in brain tissue of mice. Acetylcholine (ACh) as a neurotransmitter and acetylcholinesterase (AChE) as an enzyme that catalyzes the breakdown ACh were measured. EFAD has been proved to have the significant effects on cholinergic system by increasing ACh levles and decreasing AChE activity. In addition, EFAD improve the antioxidant system by diminishing malondialdehyde (MDA), an indicator of lipid peroxide, and elevating antioxidant such as contents of reduced glutathione (GSH) and superoxide dismutase (SOD).
Jin Yong Kang
Gyeongsang National University, Korea
Title: Relative physiological activity of chlorogenic acid and 3,5-dicaffeyolquinic acid on problems caused by high-fat diets

Biography:
Jin Yong Kang is Ph.D student at Gyeongsang National University (Jinju 52828, Republic of Korea). He is mainly studing on functional foods. He has published 2 papers in SCI(E) journals and participated as a co-author in more than 5 paper.
Abstract:
This study was performed to confirm the influence of chlorogenic acid (CGA) and 3,5-dicaffeyolquinic acid (3,5-diCQA) intake on disorders caused by high-fat diets. They have been shown to improve impared glucose tolerance and inhibit weight gain caused by high fat diet although CGA and 3,5-diCQA differ in their activity. In biopsy, it is confirmed that CGA inhibits visceral fat and liver fat accumulation, and 3,5-diCQA also inhibited visceral fat accumulation, but a 3,5-diCQA increased liver fat accumulation. Both CGA and 3,5-diCQA inhibit accumulation of visceral fat by stimulating AMPK phosphorylation in epididymal white adipose tissue. CGA also increased AMPK phosphorylation in the liver tissue, but 3,5-diCQA reduced AMPK phosphorylation. This result in liver tissue is presumed to be related to Akt phosphorylation. AKT phosphorylation was significantly increased in liver of the 3,5-diCQA group. Increased AKT phosphorylation improved glucose tolerance, but it inhibted AMPK phosphorylation and thereby increase fat accumulation in liver. In addtion, as a result of confirming the degree of liver damage, the liver fat accumulation induces oxidative stress, but 3,5-diCQA reduced oxidative damage through its antioxidant activity. To sum up, a CGA is effective in improving the problems caused by high-fat diets while maintaining balance. A 3,5-diCQA also has shown a positive effect on problems caused by high-fat diets, but it increased liver fat accumulation and thereby cause negative consequences.
Yongmin Kim
National Institute of Animal Science, Korea
Title: Effects of sex and breed on meat quality and sensory properties in three-way crossbred pigs sired by Duroc or by a synthetic breed based on a Korean native breed

Biography:
Yongmin Kim is PhD student in Bioinformatic at Kangwon National University in Korea and works at National Instititue of Animal Science as Junior Researcher. I’m interested in animal breeding and meat quality of swine.
Abstract:
This study was conducted to determine the effects of breed and sex on meat quality and sensory properties of the loin in three-way crossbred pigs: Landrace × Yorkshire × Duroc (LYD) and Landrace × Yorkshire × Woori black pig, as Korean native pig (LYW). Carcass traits did not differ by breed. Carcass weight and backfat thickness were higher in castrates than in gilts (p < 0.01). Fat content, cooking loss, and water-holding capacity were higher in LYW than LYD (p < 0.05). Redness and yellowness of the meat were higher in LYW than in LYD (p < 0.01). Further, LYW had lower pH and shear force than LYD (p < 0.001). Two sensory properties, color and flavor, were better in LYW than in LYD, and in gilts than in castrates (p < 0.05). However, other sensory traits did not differ by breed or sex. Capric acid (C10:0) was higher in LYD than LYW (p < 0.001). However, stearic acid (C18:0) and saturated fatty acid (SFA) contents were higher in LYW than LYD (p < 0.05). Eicosenoic acid (C20:2) and the n6/n3 ratio were higher in gilts than in castrates, whereas SFA content was higher in castrates than in gilts. These results suggest that certain physicochemical qualities of meat and sensory properties are improved in LYW as compared to LYD. This study provided basic data on meat quality of crossbred pigs with Woori black pig as a terminal sire.
Jan Petros J. Esparagoza
University of the Philippines, Manila
Title: An Assessment of Food Availability and Food Accessibility around a university in Manila, Philippines

Biography:
Jan Petros J. Esparagoza, a 20 year old student from the University of the Philippines, Manila, is currently undergoing his medical degree.
Abstract:
Schools are essential environments for growth and development of both students and faculties. However, there are gaps in the literature regarding food environment in the Philippines particularly around schools. As such, the study characterized the food environment around a university in Manila in terms of food availability and food accessibility.
A descriptive research design was utilized and total enumeration of food stores within three blocks from the campus was done. Food availability was assessed through direct observation using EPI Info v. 7 and was analyzed through descriptive statistics. Food accessibility was assessed using a mobile GPS application and was visualized through Arc GIS v. 10 through kernel density mapping and hotspot mapping. Buffers were also set at 100 meters and 200 meters from the centroids assigned.
High-sugar/energy-dense beverages, empty calories and canned meat products were found to be sold immediately outside the campus, and were more prevalent compared to healthier alternatives. Only a few food stores sold vegetables and fruits, with a few found immediate outside. In addition, stores that do sell fruits/vegetables were found to have only a few varieties, with only one store selling more than 10 kinds of fresh produce within 200 meters from the centroids in the campus.
Findings showed that the food environment around the university is potentially obesogenic and is less capable of providing the essential nutrients needed by an individual. Thus, a healthy food environment should be promoted within and around the university to encourage its constituents to avail of healthier food products.
Cherry Ann Garcia
University of the Philippines - Manila, Philippines
Title: Maternal Perspectives on Probiotics, Intake of Probiotic Food and Occurrence of Atopic Dermatitis among Children in Selected Urban Communities in Laguna, Philippines

Biography:
Cherry Ann Garcia is a nurse and an educator. She is passionate in conducting free public health teachings in rural communities in their province. Her expertise is on medical-surgical nursing and psychotherapy and counseling. She is a pastor’s wife.
Abstract:
INTRODUCTION:
Atopic dermatitis (AD), also known as eczema, is the most common chronic relapsing skin disease in children, affecting approximately 10% to 30% of children worldwide. For this reason, the research community investigated possible innovative prevention and treatment strategies for AD. One of these strategies was the manipulation of the intestinal flora through probiotics.
METHOD:
This study is a cross-sectional, analytic study on 680 mothers and 680 children recruited in selected urban communities in Laguna, Philippines.
RESULTS:
Most mothers (92%, n=625) had highly positive attitude to probiotics. Composite scores were computed and statistical data revealed that attitude is significantly correlated with behavioral control (r=0.2087, p-value <0.001) and intention (r=0.2934, p-value <0.001). Furthermore, it was found out that both intention (r=0.3703, p-value <0.001) and perceived control (r=0.2355, p-value <0.001) were significantly correlated to total amount of intake of probiotic food. Female children (42.43%) have higher intake than males (30.61%). High intake was also noted among children of mothers with educational attainment of Vocational (45.45%) and College and higher (41.09%); among low- (38.92%) and middle-income families (44.06%); and among those without family history of AD (38.55%). As for the frequency of consumption of probiotic food, 3 out of 10 of the respondents reported daily intake of at least one bottle of the probiotic foods enumerated. Among 680 respondents, 18.09% (n=123) were diagnosed as having atopic dermatitis, while 81.91% (n=557) were without AD. This study showed that the odds of having atopic dermatitis was 2.4 times higher among those with low intake status and 4.3 times higher among those with none intake status compared to children with high intake status.
CONCLUSION:
Intake of probiotics are positively correlated with maternal education, income, highly positive attitude, high perceived control, and intention of mothers. Consumption of probiotics is a protective factor against atopic dermatitis.
Edzil C. Besonia
Visayas State University, Philippines
Title: QUALITY ASSESSMENT OF VACUUM FRIED CASSAVA (Manihot esculenta Crantz) BITES

Biography:
Edzil C. Besonia has completed her MS Food Science and Technology in Visayas State University, Philippines.
Abstract:
Information about food composition is necessary for the assessment of diet quality, development and application of food-based dietary guidelines, providing a useful tool for the field of public health nutrition. The study was conducted to determine the effects of varying levels of frying time, cooking time and spin-drying time on the physico-chemical properties of vacuum fried cassava bites. A 33 fractional factorial experiment following Central Composite Design with different levels of frying time (30, 45, 60 minutes), cooking time (10, 15, 20 minutes) and spin-drying time (2, 4, 6 minutes) were used. Data for all experimental combinations were analyzed employing response surface regression analysis. The physico-chemical, nutritional and proximate, water activity, microbial and storage properties of the optimum process of vacuum fried cassava bites with 50 minutes frying time, 20 minutes cooking time and 6 minutes spin-drying time were evaluated.
Results on the response surface regression on the physico-chemical properties of the vacuum fried cassava bites revealed significant effects on the product except for the percent recovery and b* color unit. Optimized product has high calorie content along with the other macronutrient like fat, carbohydrates, sodium and crude protein. Storage stability of the product in the weekly monitoring of moisture content, water activity, color determination and sensorial descriptions showed significant difference over the observation period. Evaluation for total plate count and presence of yeast and molds showed a negative result.
Ronalyn V. Amper
Visayas State University, Philippines.
Title: Optimization of Bread as Influencced by Cassava (Manihot Esculenta Crantz) – Wheat (Triticum Aestivum) Combination with Alpha-Amylase and Xylanase

Biography:
Ronalyn V. Amper has completed her MS in Visayas State University, Philippines.
Abstract:
The combination action of α-amylase, xylanase and composite flour was analysed to determine their effects on the sensory quality of the bread through optimization using Response Surface Methodology (Central Composite Design). Fifteen bread formulations containing different concentrations of α-amylase (0, 0.0005 and 0.003% w/w), xylanase (0, 0.001 and 0.003% w/w) and composite flour (15, 30, 45%w/w) were used in the analysis. The study revealed that the addition of α-amylase had significant influence on the response of crust color, crumb structure, flavor and general acceptability of the bread. The presence of xylanase causes a change on the response of crust color, crumb texture and the general acceptability only, however, its crossproduct interaction with composite flour level affect the response of crust color, crumb texture and structure, flavor so as the overall acceptability of the product. The synergistic effect of α-amylase and xylanase displayed an influence positively on the overall acceptability of the bread. The optimum formulation set at acceptability rating of >7.20 on the 9-point Hedonic scale was determined at 38% composite flour with 0.002% α-amylase and 0.0012% xylanase considering a cost of P41.14 per 421.27g of loaf. The observed acceptability mean for all sensory attributes of the formulation was not significantly different with the predicted acceptability mean value of the model according to verification test. This formulation was not significantly different with the commercial bread available in the local market of Baybay, Leyte based on the consumer acceptance results with children and adults as potential target market.
Anna Monica B. Dumalaog
University of the Philippines Diliman, Philippines
Title: Assessment of Energy Balance, Nutrition Knowledge, and Attitudes of Collegiate Judo Athletes Before, During, and After Training Days
Biography:
Anna Monica B. Dumalaog is a graduate in Department of Food Science and Nutrition, University of the Philippines Diliman, Quezon City Philippines.
Abstract:
Student-athletes from weight-class sports are exposed to high pressure conditions due to heavy academic load and frequent weigh-ins. Collegiate Varsity Members drastically change food intake before weigh-ins to meet specific weight classes. Such conditions lead to the development of eating disorders and binge diets, and as a result, excess and severe depletion of nutrient stores may pose adverse health effects later on, alongside decreased athletic performance. Thus, proper nutrition management is needed. The study investigated the differences in energy balance of Collegiate Athletes before, during, and after training days. Study participants from the University of the Philippines Judo Team (n=20) completed an adapted Nutrition Knowledge, Attitudes, and Practices Questionnaires, 3-day non-consecutive 24-hour estimated food records, and 24-hour physical activity diaries before, during, and after the training days. Through the one tailed t-test (p=0.05), a significant difference was recorded between the mean caloric intake of participants from training to post training days (t=-1.967). Meanwhile, the caloric expenditure of participants were significantly higher during training days, compared to no training (t=5.03) and post training (t=6.42) days. This difference in caloric intake and expenditure may lead to athletes developing lifestyle diseases such as obesity if they decide not to pursue Judo in later life. Pearson correlation coefficient test between nutrition knowledge and attitude scores to energy balance of participants showed only minimal correlation between the variables. No correlation was found between nutrition knowledge scores and energy balance (R=0.00). On the other hand, a weak positive correlation was found between the score for sports and nutrition attitudes and energy balance. (R=0.33). Present data suggest that a more positive energy balance, or less negative energy balance can be weakly associated with increased preference towards the power and performance model of sports participation.
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Garner Algo L. Alolod
University of the Philippines Visayas, Philippines
Title: Bioactivity and Functionality of Gelatin Hydrolysates from the Skin of Oneknife Unicornfish (Naso thynnoides)

Biography:
Garner has recently completed his Masters of Science in Fisheries-Fish Processing Technology at the age of 24 years from University of the Philippines Visayas.
Abstract:
Skin gelatin of Oneknife unicorn fish (Naso thynnoides) was hydrolysed using a crude protease from Bacillus sp. under optimum hydrolysis conditions. Resulting hydrolysates was subjected to centrifugal ultrafiltration to produce fractions of ≤10KDa molecular weight. Antioxidant, antihypertensive and functional properties of the hydrolysate fraction were determined. Results showed that DPPH (a,a-diphenyl-b-picrylhydrazyl) scavenging activity (63%) and ferric reducing antioxidant power (25.90 Trolox Equivalent (mM/mg)) increased as protein concentration increased. Angiotensin converting enzyme-I (ACE-I) inhibitory activity is directly proportional to the protein concentration with highest value obtained at 33.97% and IC50 determined to be 10.17 µg /ml. Gel electrophoresis (SDS-PAGE) revealed that the gelatin hydrolysates contain mostly peptides with molecular weight ranging between 5 KDa and 30 KDa. Amino acid profile of the hydrolysates showed that it is rich in residues of glycine (Gly) (40.70%) and glutamic acid+glutamine (Glx) (25.40%). The hydrolysates was soluble over wide pH range (79.38 - 97.12%). Foaming properties increased while emulsion properties decreased as the gelatin hydrolysate concentration was increased. Results of the present study revealed that Oneknife unicornfish gelatin hydrolysates could be a potential food ingredient with antioxidant and antihypertensive properties.
Precious Dee L. Herbalega
University of the Philippines Visayas, Philippines.
Title: Screening and Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Balao-Balao, A Traditional Philippine Fermented Rice-Shrimp Mixture
Biography:
Precious Dee L. Herbalega is a graduate student in University of the Philippines Visayas, Miagao, Iloilo 5023 Philippines.
Abstract:
Lactic acid bacteria (LAB) from Philippine traditional fermented rice-shrimp mixture locally known as Balao-balao was isolated and screened. From a total of 20 selected acidproducing isolates, four isolates (LM8, LM12, LM17, and LM20) showed promising inhibitory activity against pathogens namely S. aureus, E.coli, B. subtilis and V. harveyi. Among the four isolates, LM12 showed the highest inhibitory activity. Optimum incubation period to achieve the highest activity was observed at 48 h. Isolate LM12 was identified as Pediococcus pentosaceus (Genbank Accession No. KY689941) by phenotypical tests and 16s rDNA analysis. Complete inactivation on the activity of the cell free supernatant was observed after treatment with trypsin and proteinase-K. The inhibitory activity was not affected by the addition of catalase and hydrogen peroxide, thus, the antimicrobial substance was proteinaceous in nature. Its molecular weight was approximately 5 kDa by tris-tricine SDS-PAGE. The bacteriocin of P. pentosaceus LM12 was sensitive to proteases but insensitive to lipase and amylase. It was also pH (pH 2.011.0) and temperature stable (0áµ’C-121áµ’C). After testing P. pentosaceus LM12 bacteriocin, results showed that it was active against both Gram-positive and Gram-negative bacteria. This is the first report of a bacteriocin-producing P. pentosaceus isolated from a fermented fish product with positive antagonism against Gram-positive and Gram- negative bacteria.
Nahla Mohamed Abdel khalek Khalil
Chemistry Department, American University in Cairo, Egypt
Title: Antimicrobial activity of grape seed and skin extracts coated on Corona treated LDPE and PET films

Biography:
Nahla Mohamed has completed her MS in Food Chemistry at the American University in Cairo, School of Sciences and Engineering, Chemistry Department. She is a Research Assistance at Chemistry Department, American University in Cairo.
Abstract:
Consumer demand for ready-to-eat ‘fresh’ and safe food products with less synthetic preservatives together with well documented food-borne microbial outbreaks drive both research and food industry toward new innovative methods for microbial growth inhibition while keeping food freshness, quality, and safety. Incorporation of natural bioactive agents in the packaging material to increase the shelf life of meat products is a promising technology. Grapes are of special interest because of their high content of phenolic compounds that showed antimicrobial and antioxidant effects. The aim of the present work was to investigate grape seed (GSE) and skin (GSKE) extracts’ antibacterial activity and developing bioactive LDPE/PET films that could be used as food packaging for poultry and meat products. Commercial corona treated LDPE and PET were coated with either grape seed or grape skin extract. Agar plate diffusion method was used for the investigation of the microbial properties of both extracts’ coated films against E.coli as a Gram-negative bacterium and Staphylococcus aureus as a Gram-negative one. LDPE and PET films coated with GSE showed inhibition zones of E.coli growth in the range of 16-25mm, while Staph.aureus growth inhibition zones were in the range of 15-20mm. For LDPE corona films coated with grape seed extract, the minimum inhibitory concentration (MIC) was 0.002g/1cm2 for E.coli and 0.003g/1cm2 for Staph.aureus. While for corona treated PET films/GSE, the MIC for both E.coli and Staph.aureus was 0.002g/1cm2. Corona treated LDPE and PET coated with GSKE showed inhibition zone range of 13-16.3mm with E.coli and 12-20mm with Staph.aureus. For LDPE corona films/GSKE, the minimum inhibitory concentration (MIC) was 0.0009g/1cm2for E.coli and 0.003g/1cm2for Staph.aureus. While for corona treated PET films/GSKE, the MIC was 0.002g/1cm2 for E.coli and 0.003g/1cm2 for Staph. aureus. The Total Phenolic Content of both GSE and GSKE was determined using the Folin- Ciocalteu methodology to be 315.32g (GAE)/kg, and 265.326g (GAE)/kg for GSE and GSKE respectively. The coated films; LDPE/GSE or GSKE, were used to wrap fresh chicken fillets, TVC, Pesudmonads, Brochothrix thermosphacta, Lactic acid bacteria and Enterobacteriaceae counts were determined during the storage period; 10 days for test samples and 8 days for controls. Microbiological analysis for tested samples was done on day 0, 2,4, 6, 8, and10, while for control till day 8. There was a reduction in the populations of the examined bacteria in the range of 0.2-1.4 log cfu/g in case of GSE, while with GSKE the reduction of bacterial populations range was 0.3-1.95 log cfu/g.
- Food Processing & Technology | Nutritional Epidemiology & Malnutrition | Nutrition, Health & Aging | Pediatric & Maternal | Nutrition | Public Health Research | Dietary Supplements & Functional Foods
Session Introduction
Chungkham Sarojnalini
Manipur University, India.
Title: Effect of Cooking on the nutritional properties of two small indigenous fishes of Eastern Himalayas, Manipur, India
Time : 10:00-10:30

Biography:
Chungkham Sarojnalini was born on 1st Feb. 1958. B.Sc. Zoology honour Gauhati University 1979; M.Sc. In Life Sciences Fishery specialization J.N.U Centre, Imphal 1982; Ph.D in Fish and Fisheries in Manipur University 1989. Working as an associate professor in department of life sciences Manipur University. Published 40 international and national paper in peer review journal. Her research interested areas are Biochemical aspects of fresh and processed fishes of plain and hill stream fishes. Dr. Sarojnalini was reviewer of the European food research and Technology, SPRINGER. Life member of the Journal of food Science & Technology, Mysore., Life member of the Fisheries Technologists (India), Cochin., Member, Inland Fishery Society of India., Member Zoological Society of India., Aquatic Biodiversity Conservation Society, India, Lucknow., Member Ichthyological Society Of Japan.
Abstract:
The effect of cooking methods (frying, steaming and curried) on the nutritional quality of two small indigenous fishes viz., Amblypharyngodon mola and Esomus danricus of the Eastern Himalayas were determined. Cooking significantly increased the contents of protein and ash in the two small fishes. The maximum protein content was recorded in curried A.mola (20.77%). The lowest lipid content was found in the steamed E. danricus (0.83%) and highest lipid was obtained in fried E.danricus (41.56%). A significant (p<0.05) reduction in moisture content was observed in all different cooking methods A.mola and E.danricus. The lowest moisture content was recorded in fried A.mola (22.50%) and highest moisture was found in raw A.mola (74.69%). The carbohydrate values of two raw fishes were 3.98% and 5.62%. The energy values of two raw samples were 149.2 and 143.26Kcal/100g. The higher energy values were found in fried samples of the two fishes. The studied fishes have antioxidant property and highest activity was obtained in curried A.mola (0.11μg/ml). The ranges of polyunsaturated fatty acids such as Docosahexanoic, Eicosapentaenoic, Arachidonic, Linolenic and Linoleic acids were 0.12-2.91mg/100g, 0.04-0.73mg/100g, 0.07-2.03mg/100g, 0.22-3.7mg/100g and 1.01-2.30mg/100g respectively. The results indicate that cooking methods had considerable effects on proximate composition, antioxidant property and polyunsaturated fatty acids. The study showed that the small indigenous fishes are good sources of protein, energy, polyunsaturated fatty acid and antioxidative compounds. Therefore consumption of small size fishes could found high energy and prevent the deficiency of malnutrition, cardiovascular disorders and brain function in the developing countries, because the ratio of n:3/n:6 is a marker of the biomedical significance for fish oils.
Khairul Bariah Sulaiman
PhD student at Universiti Sains Malaysia
Title: Effect of different fermentation duration of Malaysian cocoa beans on volatile compounds
Time : 10:30-11:00

Biography:
Khairul Bariah is working at Malaysian Cocoa Board for almost 15 years. Currently she is pursuing PhD in food processing at Universiti Sains Malaysia.
Abstract:
Fermentation is an important process in cocoa that enable the formation of flavor precursor. The process is influenced by various factors, in which will eventually determine the final flavor quality of dried cocoa beans. Therefore, this study was to evaluate the effect of different fermentation duration using a shallow box on the volatile compounds of the dried cocoa beans. The fermentation was conducted at the Cocoa Research and Development Center, Bagan Datuk using 150 kg of fresh cocoa seeds. During fermentation, 15 kg of wet beans was randomly taken out at 0, 24, 48, 72, 96 and 120 hours of duration and subsequently sun-dried until the moisture content reduced to 7.5%. The volatile compounds of cocoa powder prepared from samples were extracted by Solid Phase Micro Extraction using 65μm Polydimethylsiloxane-divinylbenzene coating fiber and analyzed in Gas Chromatography system equipped with Mass Spectrometer Detector. The study identified a total of 126 compounds in the 6 samples of dried cocoa beans which classified into 20 groups. Esters, acids, hydrocarbons, ketones and pyrazines were presented as the main volatiles with more than 10 compounds in each groups. Cocoa beans which fermented for 48 hours using shallow box exhibited highest number of volatiles which associated with cocoa specific aroma such as ethyl laurate, phenethyl acetate, ethyl cinnamate, methyl cinnamate,isoamyl acetate, octanoic acid, a-ethylidene benzeneacetaldehyde, benzeneacetaldehyde and phenylethyl alcohol.
Claude Billeaud & Wafae Belcadi
University Hospital Center of Bordeaux, France
Title: The influence of lipids intake of Lactating women diet and those of the Own Human Milk in France
Time : 11:20-12:20

Biography:
Claude Billeaud received his MD degree from the Medical University of Bordeaux ( France) in 1979 after a graduation in human cytogenetics (1976). He then studied pediatrics and has been the Clinical Assistant Director of Bordeaux University in the departments of Pediatrics, Neonatology and Intensive Care since 1983.
He currently serves as a pediatrician in the neonatal unit at the Children’s Hospital of Bordeaux, as a scientific manager of Bordeaux-Marmande human milk bank, as a lecturer and head of research (HDR : Habilitation to direct research) in neonatal nutrition at the Medical University of Bordeaux.
His particular interest in research led him to graduate in Biology and Health (1988, Bordeaux), be awarded a master in statistics applied to clinical research ( 1991, Montreal) and complete a PhD in nutrition and food science (2000, Bordeaux).
Along his career he has often been invited as a guest professor specialised in nutrition and neonatology in various universities abroad ( Montreal, Corrientes in Argentina).
Over the last 35 years, he has been an active member of different scientific organisations, either French, European or American, specialised in perinatal medicine (neonatology, pediatrics and nutrition). In this instance, he has served as the President of the Association for Pediatric Education in Europe (A.P.E.E) since 2008 and behalf APEE he is Member of European Academy of Paediatrics (EAP).
He has also been very involved in the French human milk banking association (ADLF) for more than 10 years, sharing his academic knowledge focused in nutrition and his long clinical experience in neonatology. He is currently carrying out several researches on the composition of human milk.
As an expert in nutrition and perinatal medicine, he is also the author and co-author of numerous scientific publications.
Abstract:
Feeding lactating women and composition of Human milk (HM) from 1997 to 2014 in France
1) INTRODUCTION / OBJECTIVE: The latest recommendations to increase intakes of polyunsaturated fatty acids (PUFA) n-3. Indeed, linoleic acid (LA) and α-linolenic acid (ALA) are the essential precursors of long chain PUFA respectively, arachidonic acid (ARA) and docosahexaenoic acid (DHA), which are essential for cognitive development of the newborn. Furthermore the LA excess foster obesity. This study looks at the monitor French consumption of PUFA from 1997 to 2014, following the fatty acid composition (FA) of HM that reflects FA consumption of the lactating mother.
2) MATERIALS and METHOD: Samples of mature HM was collected in the morning between 1 and 3 months post partum across 4 clinical trials conducted in 1997 (n = 16 HM), 2007 (n = 142), 2012 (n = 22) and 2014 (n = 80). The FA composition of HM was determined by direct transesterification and analyzed by GC-FID and compared by ANOVA followed a testing and Kruskall Wallis. Dietary survey were analyzed by the BILNUT software.
3) RESULTS:
The data acquired in France between 1997 and 2014 have shown that the rate of ALA increased 84% and those in LA were down 25%. These changes induce a decrease in the ratio LA / ALA 60% (27.6% in 1997- 10.7% in 2014) . DHA rate improved by 13% between 2007 and 2014 (0.24% to 0.29% of FA in 2014) whereas the ARA is stable. Potentially harmful trans FAs rates have fallen by 50% since 1997 following the improvement of the quality of French margarines. Dietary surveys have shown a 25% decrease in energy intake in 17 years, a deficiency in calcium, zinc, Magnesium, vitamins A, E, D, B5, B6, B9.
4) CONCLUSION : The studies conducted since 1997 have shown a modification of the content of n-3 and n-6 ​​of the HM, reflecting changes in feeding habits, closer to the current recommendations. In this context, should be promoted firstly declining ratio LA / ALA (5-10) by the consumption of oil or margarine rich in ALA to optimize biosynthesis of ALA to DHA and DHA provide directly by the consumption of fatty fish.
Thilini Keerthirathne
Flinders University, Australia
Title: Investigation into the effect of pH and temperature on the survival of Salmonella enterica serovar Typhimurium
Time : 12:20:12:50

Biography:
Thilini Keerthirathne is currently a PhD student in the College of Science and Engineering, Flinders University, South Australia. She has obtained her B.Sc. in Genetics from the University of Bangalore, India in 2010 and completed her postgraduate studies in Biochemistry and Molecular Biology at the University of Colombo, Sri Lanka. She was working as a research assistant at the National Institute of Fundamental studies, Kandy, Sri Lanka before coming to Australia. She has published four peer-reviewed journal articles, one extended abstract, 4 conference abstracts and one conference poster in national and international conferences.
Abstract:
In Australia the incidence of salmonellosis is increasing with Salmonella Typhimurium linked to eggs and raw egg products identified as the main cause. Australian guidelines recommend raw egg mayonnaise is prepared and stored under 50C and adjusted to a pH of 4.6. This study investigated the combined effect of pH and temperature on the survival of S. Typhimurium. Nutrient broths with a pH of either 4.2, 4.4, 4.6, or 7.0 (adjusted with acetic acid) were inoculated with 1 mL log phase S. Typhimurium (105 CFU/ mL) and were incubated at either 370C, 230C or 40C. All experiments were conducted in triplicate. The survival of Salmonella over time was determined using the drop plate method on X.L.D. agar, also conducted in triplicate. Survival was significantly (P<0.05) improved when cultures were incubated at 40C compared to 230C and 370C for all pH conditions tested. After 24 hours of incubation at 370C, there were no viable Salmonella at pH 4.2 and 4.4 but there was survival at 230C and 40C for the same pH conditions. The maximum survival was at 4 0C. In conclusion, lower temperatures protected S. Typhimurium from the bactericidal effect of low pH.
Xiaoxia Dai
Xi′an Jiaotong University Health Science Center, China
Title: Selenium increase the clearance of immune complexes in Kashin-Beck Disease, an endemic osteochondropathy
Time : 13:40-14:10
Biography:
Xiaoxia Dai has completed her PhD in the year of 2006 from Xi’an Jiaotong University and postdoctoral studies from University of Iowa Carver College of Medicine, Department of Microbiology and Immunology. Now she is the researcher and teacher in School of Public Health, Xi′an Jiaotong University Health Science Center. She published more than 20 papers and has been serving as a member of the society of trace elements in shaanxi province.
Abstract:
Many studies suggest that adequate intake of Se is required to ensure optimal immune function and to prevent malignancy. Various components of the immune system fail to function correctly if there is dietary Se deficiency. In the present study, the effect of selenium supplements on the clearance of immune complexes of patients with KBD was evaluated. The effect of different selenium preparations on (1) erythrocyte selenium content, (2) activities of glutathione peroxidase, (3) erythrocyte immune adherence function, and (4) circulating immune complexes (CIC) content of patients with KBD was determined. The effect of sodium selenite and selenium yeast were also compared.
In this randomized study, fifty Kashin-Beck disease patients from the endemic area, aged 13-16 years, were divided into two groups and were given either selenium yeast or sodium selenite orally to provide 200µg of selenium per day for 12 weeks. After supplementing with selenium for 12 weeks, erythrocyte selenium level, glutathione peroxidase activity, and E-C3bRR showed significantly high values, the difference in E-ICRR content was not significant. The effect of selenium in enhancing blood selenium levels and the clearance of immune complexes is greater when supplements provided as selenium yeast is used as compare to sodium selenite. This work was supported by the National Nature Scientific Foundation of China (Grant No. 81673117 and 81573140).
Thunnalin Winuprasith
Institute of Nutrition, Mahidol University, Thailand.
Title: Development of Healthy Pumpkin Pudding for Elderly with Different Degrees of Tooth Loss
Time : 14:10-14:40

Biography:
Dr. Thunnalin Winuprasith* is a faculty member of Institute of Nutrition, Mahidol University. She got a master’s degree and Ph.D. from Institute of Nutrition and Faculty of Science, Mahidol University, respectively. Her research interest focuses on food product development, food emulsions and encapsulations for nutrition aspect.
Abstract:
The aim of this research was to develop pumpkin puddings which were suitable for elderly in term of texture and nutritional quality. The dessert contains milk protein, pumpkin powder (PP), modified starch, vegetable oil, carrageenan, and sugar. Subjects were grouped into three categories by posterior occlusal contact, according to the Eichner Index. Group A had contacts in four support zones; group B had one to three zones of contact or contact in the anterior region only; and group C had no support zones at all, although a few teeth could still remain. The levels of PP added were varied at 4.0, 6.0, and 8.0% (w/w). The textural parameters and color significantly affected by PP concentration. From sensory evaluation, the suitable PP and carrageenan levels were found to be 6.0% PP with 0.2% carageenan for group A, and 8.0% PP with 0.3% carageenan for group B and C. For group A, the hardness, adhesiveness, cohesiveness, and guminess were 351.33 g, 151.40 g.sec, 0.46, and 41, respectively. For group B and C, the hardness, adhesiveness, cohesiveness, and guminess were 95.89 g, 105.78 g.sec, 0.44, and 9.34, respectively. The energy distribution of macronutrients including protein, fat, and carbohydrate for all formulations were about 10-15, 25-30 and 55-65%, respectively. In addition, the pumpkin pudding with 8.0% PP contained 1.60 g total dietary fiber (per 100 kcal), which was classified as a source of dietary fiber. This product could be used for improveing nutritional status of elderly.
Numphung Rungraung
Institute of Nutrition, Mahidol University, Thailand.
Title: Development and characterization of texture-modified pork ball for elderly
Time : 14:40-15:10

Biography:
Numphung Rungraung has completed her Master’s degree in Food and Nutrition for Development, Institute of Nutrition, Mahidol University. She is a researcher of Center of Innovation and Reference on Food for Nutrition (CIRFON), Institute of Nutrition, Mahidol University. Her research interest focuses on formulation of food products for nutrition purposes, food processing and storage on antioxidant activity and physicochemical properties of food products.
Abstract:
In the elderly population, one of the restrictive factors in food consumption may be that some foods become troublesome to eat as losing their teeth and swallowing dysfunction at an elderly age. The purpose of this work was to develop texture-modified pork ball with varying level of pork (30-38%), lard (20-24%), and tapioca flour (1.8-3.4%) added. All formulated products were investigated in terms of textural properties and sensory evaluation. The result showed that hardness, springiness, cohesiveness, gumminess, chewiness increased whereas adhesiveness decreased, when the pork level increased and the lard and flour decreased. Sensory evaluation was performed by elderly subjects using 5-points hedonic scale and 5-point just-about-right. The subjects were grouped into three categories by posterior occlusal contact, according to the Eichner Index. Group A had contacts in four support zones; group B had one to three zones of contact or contact in the anterior region only; and group C had no support zones at all, although a few teeth could still remain. The result indicated that all developed formulas of texture-modified pork ball had just about right in color, taste and easy to chewing and overall liking score was in the range of like slightly to like moderately. From sensory evaluation, the suitable level of each ingredient was found to be 38% pork, 20% lard and 1.8% flour for group A, and 34% pork, 22% lard, and 2.6% flour for group B and C. This product combinated with other foods could be used for improveing nutritional status of elderly.
Iman Mirmazloum
Researcher in Kaposvár University, Hungary
Title: Enzymatic Degradation of Sodium alginate, Chitosane and Pectin resulted in oligosaccharides with boosted prebiotic index
Time : 15:10-15:40

Biography:
Dr. Iman Mirmazloum has expertise in medicinal plants, natural product supplementation and novel food products. His main research area is on molecular mechanisms of plant secondary metabolites biosynthesis. Iman is currently involved in interesting research projects in Kaposvár University (Hungary) to develop new functional food products with enhanced health promoting properties. He is going to present a report (poster) about enzymatically produced Sodium alginate, Chitosane and Pectin oligosaccharides with boosted prebiotic index.
Abstract:
Scope of the research: Prebiotics are getting more and more into the forefront of interest of researchers and suppliers, as these non-digestible polysaccharides are of justified health promoting impact due to the facilitation of the growth of certain beneficial, health-associated gut bacteria. Their positive physiological effects might efficiently be utilized by several segments of the food industry. Sodium alginate, chitosane and pectin are commonly used polysaccharide molecules in relevant food industrial application. The scope of this research was to increase their biological value by enzymatic digestion and enhancing their prebiotic activity.
Methodology & Theoretical Orientation: Sodium alginate, chitosan and pectin have been subjected for enzymatic degradation by Pectinase, Lactase, β-Galactosidase, Glucosidase and two commercial blended enzymes mixture to produce their corresponding oligosaccharides with plausible prebiotic effect. The enzymes have been studied for their optimum reaction condition (pH and temperature) and consequently their potential activity on breaking the tow polymers. The reaction products were then subjected for both analytical and microbiological assays, in order to establish the quality, quantity, and the prebiotic effect of degradates, respectively. The Prebiotic index of the obtained oligosaccharides has been assessed using Lactobacillus delbrueckii subsp. Bulgaricus, and Lactobacillus casei 2756. Casei, as bacterial representatives.
Findings: Thin layer chromatography revealed the enzymatic degradation pattern of the studied macromolecules. The results showed an enzyme quantity oriented reaction rather than time or substrate concentration oriented interaction. Almost the same amount of hydrolysates has been released from 0.1 and 1% pectin solutions as a result of the enzymatic hydrolysis from the carbohydrate polimers. The highest prebiotic indices have been established for the pectin degradates yielded by pectinase, and the alginate oligosaccharides after 24h co-culture with glucosidase enzyme. Pectin and chitosane degradates also enhance significantly the bacterial culture. It can be concluded that the hydrolysed polysaccharides can be applied as prebiotic elements to be supplemented in functional foods.
Niramol Muangpracha
Institute of Nutrition, Mahidol University, Thailand
Title: Effect of microfibrillated cellulose extracted from mangosteen rind on physicochemical and sensory properties of modified-fat ice cream

Biography:
Niramol Muangpracha is a research staff of Institute of Nutrition, Mahidol University. She got a Bachelor's degree of science from Burapha University. Her research interest focuses on food product development for nutrition.
Abstract:
The aims of this study were to study the effect of microfibrillated cellulose (MFC) concentration on physicochemical and sensory properties of modified-fat ice cream. Aqueous MFC was prepared by extraction of ground mangosteen (Garcinia mangostana L.) rind with hot sodium hydroxide and subsequent shearing in a high pressure homogenizer. The full-fat (12% fat content) chocolate ice cream using egg yolk as an emulsifier was used as a reference (R). Modified fat ice cream was formulated by using MFC to replace egg yolk and fat at 3.0, 6.0, and 9.0% by weight, which were labeled as low-fat, light, and reduced-fat, formulas, respectively. For physicochemical properties, there were significantly increased in pH, overrun, and viscosity correlated to the increment of MFC concentration. Melting rate and lightness were decreased at higher concentration of MFC. In contrast, MFC did not significantly affect °Brix, hardness, air cell size, and fat destabilization in modified-fat ice cream. Nutritional values showed decreased in energy and fat content in modified-fat ice cream. There were increased in dietary fiber and moisture content in modified ice cream. From sensory evaluation, the optimum level of MFC was found to be 6.0%. The product was accepted by the panelists with the overall acceptability score between like slightly and like moderately. This study provided information that can be used as application of MFC in food products.
Fatima bi
Dept of Home Science, Berhampur University, India.
Title: Preference of Food Intake among College Girls and Their Knowledge about the Ill Effects on Health
Biography:
Fatima bi is 24 yrs old, and completed her M.Phil in Home Science from Berhampur University Odisha country India, she has published 6 papers and one book till date.
Abstract:
Good food good life as for human being and every living organism food is the first priority for stay and feet in daily life. Adolescent is a very crucial stage of lifespan as compare to other ages because body primary and secondary sex characteristics are develop in the stage of adolescent and early puberty, good nutrition and healthy diet needed to prevent deficiency diseases. Due to the influence of modernization, urbanization and mass media the college girls more attractive for eating oily and spicy food like Chowmin, Berger, Ghupchup, pizza, French fries, chilli chicken, chicken pakoda etc, they don’t know about the ill effects on health and some time never check the hygiene and cleanliness. Poor intake of food associated with health problem like vomiting, gas, obesity, hypertension, nutrition deficiency diseases etc, girls not interested for eating healthy food like fruits, milk, egg and vegetables, but as for survive a healthy life need to take a full plate of nutrition. For the purpose of the study highlight some important objective – to analyse the dietary pattern of the college girls, to study the fast food intake , to analyse the knowledge regarding ill effects on health, and to collect some data regarding their parents attitude and view for eating fast food as for data collection taking 100 college girls from government women’s college sambalpur by applying purposive random sampling, and using self prepared questionnaire and discussion method for fill up the questionnaire and by using statistical techniques conclude the problem solution.
Brajesh Kumar
Indian Institute of Technology Kharagpur, India.
Title: Effect of microwave assisted soaking on hydration and gelatinization properties of paddy during parboiling
Biography:
Abstract:
Parboiled rice is preferred over raw rice because of its high nutritional content and better cooking property. But, the process of parboiling is time consuming and also requires precise control to ensure the desired grain quality. The current study is based on microwave soaking of paddy for subsequent parboiling. This study investigated the effect of microwave treatment along with varying grain weight and grain to water ratio. The moisture uptake, starch gelatinization, leaching losses, and swelling index were evaluated for the experimental conditions. The microwave power was varied from 400 W to 900 W and soaking time was maintained between 5 to 25 min. A face-centred central composite design was applied and a total of 30 experimental runs were generated. During soaking, with increasing grain weight, the energy utilization efficiency was found to be higher. Higher grain water ratio was more effective for moisture uptake, only at longer soaking duration. Leaching loss was increased with increase in soaking time up to microwave power of 630 W, beyond which the rate of leaching goes down. Swelling was more prominent once the moisture content exceeds 24% mc (wet basis), wherein the major increase was noticed in the width of the grain. A partial gelatinization was noticed when the grain was soaked for longer duration under higher microwave power. The findings of the study would help in reducing the soaking duration and preventing the undesirable changes in the process of parboiling.
Gayatri Mishra
IIT, Kharagpur, India.
Title: Detection of Rhyzopertha dominica Infestation in Stored Wheat Grain Using Near Infrared Hyper-spectral Imaging

Biography:
Gayatri Mishra has completed her Masters in Food Processing Technology in Anand Agricultural University, Anand, India.
Abstract:
Insect infestation in stored wheat grain is a potential hazard to the consumers and also fetching high economical loss to the farmers and food industries. Rhyzopertha dominica (lesser grain borer) is one of the most common insect grows over stored wheat grain causes major quantitative loss by feeding on the endosperm and also contaminate the grain by releasing excreta. High demand for zero contamination in food grain leads to advance analytical techniques for detection of insect infestation with high accuracy. Hyperspectral transmission images were acquired from normal and insect-damaged wheat grain over the spectral region between 400 nm and 1000 nm for 100 kernels. Ten statistical image features maximum, minimum, mean, median, standard deviation, and variance) and 10 histogram features were extracted from images. The statistical discriminant classifier namely support vector machine (SVM) was used to detect lesser grain borer infestation. Principal component analysis was used for wavelength selection; two wavelengths 900nm and 1000 nm corresponding to highest factor loading were found to be most significant for classification. SVM analysis correctly classified 93% of healthy and insect infested kernels at 1000nm wavelength region. The study demonstrated hyperspectral imaging can efficiently detect the insect infestation in stored wheat grain and can be used in food industries for rapid and online detection of stored wheat defects.
Fatima bi
Dept of Home Science, Berhampur University, India.
Title: Preference of Food Intake among College Girls and Their Knowledge about the Ill Effects on Health
Biography:
Fatima bi is 24 yrs old, and completed her M.Phil in Home Science from Berhampur University Odisha country India, she has published 6 papers and one book till date.
Abstract:
Good food good life as for human being and every living organism food is the first priority for stay and feet in daily life. Adolescent is a very crucial stage of lifespan as compare to other ages because body primary and secondary sex characteristics are develop in the stage of adolescent and early puberty, good nutrition and healthy diet needed to prevent deficiency diseases. Due to the influence of modernization, urbanization and mass media the college girls more attractive for eating oily and spicy food like Chowmin, Berger, Ghupchup, pizza, French fries, chilli chicken, chicken pakoda etc, they don’t know about the ill effects on health and some time never check the hygiene and cleanliness. Poor intake of food associated with health problem like vomiting, gas, obesity, hypertension, nutrition deficiency diseases etc, girls not interested for eating healthy food like fruits, milk, egg and vegetables, but as for survive a healthy life need to take a full plate of nutrition. For the purpose of the study highlight some important objective – to analyse the dietary pattern of the college girls, to study the fast food intake , to analyse the knowledge regarding ill effects on health, and to collect some data regarding their parents attitude and view for eating fast food as for data collection taking 100 college girls from government women’s college sambalpur by applying purposive random sampling, and using self prepared questionnaire and discussion method for fill up the questionnaire and by using statistical techniques conclude the problem solution.
Brajesh Kumar
Indian Institute of Technology Kharagpur, India.
Title: Effect of microwave assisted soaking on hydration and gelatinization properties of paddy during parboiling
Biography:
Abstract:
Parboiled rice is preferred over raw rice because of its high nutritional content and better cooking property. But, the process of parboiling is time consuming and also requires precise control to ensure the desired grain quality. The current study is based on microwave soaking of paddy for subsequent parboiling. This study investigated the effect of microwave treatment along with varying grain weight and grain to water ratio. The moisture uptake, starch gelatinization, leaching losses, and swelling index were evaluated for the experimental conditions. The microwave power was varied from 400 W to 900 W and soaking time was maintained between 5 to 25 min. A face-centred central composite design was applied and a total of 30 experimental runs were generated. During soaking, with increasing grain weight, the energy utilization efficiency was found to be higher. Higher grain water ratio was more effective for moisture uptake, only at longer soaking duration. Leaching loss was increased with increase in soaking time up to microwave power of 630 W, beyond which the rate of leaching goes down. Swelling was more prominent once the moisture content exceeds 24% mc (wet basis), wherein the major increase was noticed in the width of the grain. A partial gelatinization was noticed when the grain was soaked for longer duration under higher microwave power. The findings of the study would help in reducing the soaking duration and preventing the undesirable changes in the process of parboiling.

Biography:
Prof. Afrozul Haq has completed his Ph.D. at the age of 29 years from the Aligarh Muslim University, Aligarh. He did his postdoctoral studies at Institut de la Sante et de la Recherché Medicale (INSERM), and Foundation for Medical Research, Paris, France. More than 35 years of experience as a basic & clinical research scientist working in research labs, and hospitals around the world including Pasteur Institute, Paris, France; McGill University, Montreal, Canada; King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia. Over 150 publications in peer review journals. Prof. Haq is serving as Managing Guest Editor of the Journal of Steroid Biochemistry and Molecular Biology and as member of Editorial Board of several research journals.
Abstract:
Obesity is a significant health problem world-wide and the increase in obesity rates presents a major public health concern. Vitamin D deficiency is another pandemic and has been implicated in several diseases including obesity, cardiovascular risk, metabolic syndrome and many non-skeletal diseases. The role of vitamin D in calcium and bone metabolism is well established. Hypovitaminosis D is well-documented in those who have had bariatric or gastric bypass procedures, in which a malabsorptive state is deliberately induced, but there is no evidence that obesity itself results in reduced absorption of dietary vitamin D. Given that vitamin D is fat-soluble and that calcium absorption has been shown to be increased in diets high in fats, it is unlikely that obesity affects vitamin D-calcium homeostasis through altered gut absorption. The association between reduced 25(OH)D concentrations and obesity is well established. Correction of low 25(OH)D concentrations in obese individuals requires higher doses than those often advocated for the general population (may be the Endocrine Society Guidelines for Vitamin D Supplementation). There are plausible mechanisms and some in vitro evidence supporting a role for vitamin D in weight reduction, with the proviso that it may be difficult to determine which effects are due to vitamin D itself and which are mediated via calcium. There is a clear need for adequately-powered, prospective interventions which include baseline measurement of 25(OH)D concentrations and involve adequate doses of supplemental vitamin D. Until such studies have been reported, the role of vitamin D supplementation in obesity prevention remains uncertain.
Amit Bahukhandi
G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand (India)
Title: Variation in polyphenolics and Antioxidant Activity of Traditional Apple Cultivars from West Himalaya, Uttarakhand

Biography:
Mr. Amit Bahukhandi has submitted his Ph. D. degree from Uttarakhand Technical University, Dehradun, Uttarakhand (India) entitled “Altitude and habitat dependent variation in morphological and phytochemical diversity of selected Apple (Malus domestica Borkh.) cultivars in Uttarakhand”. During his Ph. D., his involvement was largely focused on quantification of secondary metabolites of different apple cultivars and investigated that how environmental and edaphic factors play role in secondary metabolites accumulation. Based on this work he has received “Young Scientist Award” in the 10th Uttarakhand Science Congress, Dehradun in 2016. In addition, he also worked on the chemical characterization and antioxidant activity of some threatened medicinal plants of Himalaya. Mr. Bahukhandi has published over 14 research paper in different National and International Journals of repute in addition to several popular articles and many conference presentations. Presently, he is focusing in “Forest Resource and Plant Biodiversity” under the National Mission For Sustaining The Himalayan Ecosystem at G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand (India).
Abstract:
Fruits are reported rich source of health benefit compounds, such as polyphenols and antioxidants. Regular consumption of fruits is known to reduce the risk of several degenerative diseases including cardiovascular and cancer. Polyphenols, which naturally present in fresh apples (Malus x domestica), are widely acknowledged as an antioxidant. However, the biochemical attributes of the cultivars of apple from west Himalaya are not systematically explored. In the present study, attempts were made to quantify the polyphenolic content and antioxidant activities in fully ripened fruits of 3 traditional apple cultivars namely Benoni, Fanny and Rymer growing at different locations of Uttarakhand (west Himalaya), India. Results revealed that phenolic (0.94-7.00 mg GAE/g fw), flavonoid (1.02-9.86 mg QE/g fw), flavonol (0.77-6.92 mg CE/g fw), tannin (15.49-37.99 mg TAE/g fw) and antioxidant activity (ABTS- 0.31-6.74; DPPH-0.50-1.41; FRAP-0.77-3.92 mM AAE/100g fw) varied significantly among the cultivars and the maximum content was recorded in Rymer fruits of Mukhwa location as compared to others. Altitude showed significant (p<0.05) positive relationship with total phenolic (r=0.895), flavonoid (r=0.843), flavonol (r=0.812), ABTS (r=0.856) and FRAP activity (r=0.830). Based on the results, it is concluded that Rymer is one of the most promising source of polyphenolic content and antioxidant activity and therefore, recommended for mass plantation at suitable localities for harnessing maximum potential.
Anggi Laksmita Dewi
Universitas Gadjah Mada, Indonesia.
Title: Sub-Acute Effects on Lactobacillus plantarum Dad-13 Fermented Soy Milk on Lipid Profiles and Blood Pressure in Hypercholesterolemia Rats

Biography:
Anggi Laksmita Dewi has recently completed her Bachelor's degree in Nutrition and Health at Universitas Gadjah Mada. She is passionate about food and nutrition also their relation to molecular biology and genomics.
Abstract:
Hypercholesterolemia is risk factor of many disease. Lipid profile degeneration and high blood pressure may lead to earlier death. Processed soy products, namely soy milk, contains high isoflavones levels which usually can be used as antioxidants. However, community acceptance of soy milk in Indonesia is very low because the taste is less favorable. Fermentation can be done to improve taste and flavor of soy milk. Fermented soy milk with Lactobacillus plantarum Dad-13 is believed to help in managing blood profile and blood pressure. Method: Eleven male Sprague dawley rats is induced with high-fat diet, which has been made from a mixture of 80% A.D.II standard diet, 15% melted butter, and 5% quail egg yolks, in 2 weeks. Lactobacillus plantarum Dad-13 fermented soy milk is given 1,8ml/day to treatment group for the next 5 days. Measurement of blood pressure and blood lipid levels were performed at the end . ANOVA is used to analysis each parameter with p<0.05 is considered significant. Results: After treatment for 5 days, there’s no significant changes in cholesterol, tligliserida, HDL and LDL levels (p> 0.05). Blood pressure hasn’t been found significant change in each group (p> 0.05). Conclusion: Induction of Lactobacillus plantarum Dad-13 fermented soy milk for 5 days has not had a significant effect on lipid profile and blood pressure in hypercholesterolemia rats.

Biography:
Mahrus Aldiansyah is student of Jember University, Indonesia. I am still eighteen years old, I have joined some competition of writing paper in public health topic. I hope can join this conference in order to increase my experience in public health discussion.
Abstract:
In the era of modern society as a conceptual that good food is food that is practical. Society is always busy with a variety of activities that should be undertaken, in both the formal or informal sector. The existence of the bustle to the pattern or style to eat in everyday life. Fast food become the premier choice for those without considering the health elements. Today's society is very difficult to avoid fast food. Modern lifestyles create a society began to get used to the fast food, the reason the community choose fast food is a way of presenting quick and easy to be consumed for everyone because it can be consumed while activities such as walking, travelling etc. Fast food is so popular among the public, but keep in mind that behind the fast food that looks delicious and seductive turns out there earlier than the onset of various diseases. The biggest cause of death in Indonesia is high which is equivalent to kolestrol mortality kolestrol in America. The majority of Indonesia society makes the basic food of animal protein which also serve as a benchmark a healthy community and meet the kebutuhun of the body. Therefore, created innovative food products that are rich in vegetable proteins with utilization of Tempe as a main ingredient which is Practical, economic, Food and Taste the legendary Zero Cholesterol Food-based, with some reason to give the community a sense of kecintaaan food is practical but also healthy and economical because it uses ingredients that are already well-known among the public also nourish i.e. tempe which is cholestrol zero food food. Kedepanya the concept of refined this tempe made "Rice Roll Tempe".
Buavaroon Srichaikul
Mahasarakham University,Thailand.
Title: Bioactivity values with ultrasonication extraction and acute toxicity of Nelumbonucifera Linn. flower extract for anti aging drink
Biography:
Buavaroon Srichaikul is a Faculty of Public Health, Department of Nutrition, Mahasarakham University, Mahasarakham, Thailand.
Abstract:
Health drinks currently are of popular demand globally. This study aimed to evaluate bioactivity quantities , toxicity, total phenolic contents, different extraction methods between Ultrasonication , maceration with 40% and 50% ethyl alcohol during period of time. In Thailand the production of anti aging Flower extract health do not meet the standard required by Thai FDA. Therefore Nelumbo nucifera Linn .flower extract were tested for antioxidant activities with DPPH, FRAP ,ABTS ,total phenolic, HPLC. The study was found that the Nelumbo nucifera Linn flower extract drink consisted of high contents of ferulic acid, rutin and catechin confirmed by HPLC. The result of acute toxicity analysis of Nelumbo nucifera Linn flower extracts in albino vista rats showed no sign of death or abnormality in abino vista rats which could indicate no acute toxicity in Nelumbo nucifera Linn flower extracts.
Wilda Yunieswati
Bogor Agricultural University, Indonesia.
Title: Relationship between nutritional status, health status, food consumption, and lifestyle to work productivity of cocoa farmer in Polewali Mandar, West Sulawesi

Biography:
Wilda Yunieswati is a post-graduate student in Department of Community Nutrition, Bogor Agricultural University, Indonesia. She gets the scholarship from Indonesia Endowment Fund for Education for her master study in Bogor Agricultural University, Indonesia. She has her expertise in community nutrition and passion in improving the nutrition, health, and wellbeing. She has an experience in public community activity especially about children education and children nutrition. She joins some of public community in Bogor, Indonesia to improve the nutritional and health knowledge of school-age children and hope they can get better nutrition, health, and life in the future.
Abstract:
Statement of the Problem:
Labor from agricultural sector is the largest labour force in Indonesia, which reached 32.61% of Indonesian total labor force, but the agricultural sector only contributed 14.43% of total GDP. This condition indicates that work productivity in agriculture sector is low. Cocoa is one of the leading commodities in plantation sub sector. Cocoa development has a potential part in the economy development and contributes as the third largest Indonesia’s foreign exchange after palm oil and rubber. One of the way to improve the productivity of cocoa is give an attention about the good nutrition and good health of the cocoa farmer. Nutrition and health status have been widely known as a factor to improve work productivity of farmers.
Methodology & Theoretical Orientation:
The subjects of this study were male cocoa farmers in two districts of Polewali Mandar, West Sulawesi, Indonesia aged 18-65 years old. Data collected by interview with questionnaire and measured directly for anthropometry status.
Findings:
The result of this study shows that there is no relationship between nutritional status, food consumption and lifestyle to work productivity, but there is a relationship between health status (blood pressure) and amount of cocoa picked per day (kilograms) and also health status (central obesity) and number of absent day to plantation due to sick. Besides that, there is no relationship between poor diet and health status (hypertension and central obesity) in male cocoa farmers. In this study, variables that influence the hypertension are exercise (OR: 3.836), genetic (OR: 312) and smoking habit (OR: 0.206). Then, variables that influence the central obesity are energy adequacy (OR: 9.64), exercise (OR: 5.0) and protein adequacy (OR: 1.58).
Basma Nejjar
Faculty of medicine in Liege Belgium and Faculty of Sciences in Kenitra Morocco.
Title: Typology of food consumption and serum level of Phosphocalcic biomarkers among Moroccan women

Biography:
I am Nejjar Basma, 27 years old, Moroccan joint Ph.D. student between Faculty of medicine in Liege Belgium and Faculty of Sciences in Kenitra Morocco. My thesis project is about the status of Vitamin D among Moroccan women. In this sense, I took three epidemiological studies from 2013 till 2015, I have gathered 1488 data concerning women from the general population and ambulatory medicine and I published five articles in reputed journals.
Abstract:
Actually, Moroccan is undergoing an epidemiological and nutritional transition. This shift from traditional diets to Western-style diets has been a key contributor to many diseases affecting the general health of a moroccan individual such as the phosphocalcic metabolism. This study aimed at describing the typology of food consumption and to highlight the difference of serum level of phosphocalcic biomarkers according to this typology of consumption among Moroccan in Meknes region. This cross-sectional study was taken from February to March 2015 and gathered 116 subjects 18-70y. Dietary intake was assessed by a validated a frequency consumption questionnaire; the sun exposure per week( less versus more than 15 min) was recorded and a blood sample was taken in order to assess the serum level of biochemical markers (calcemia, phosphatemia and 25 hydroxyvitamin D (25OHD)). The solar exposure was used as an adjustment factor. The principal component analysis showed two profiles of the typology of food consumption: one rich in vegetal and cereal products and other rich in animal products. Calcemia and 25OHD were different according to animal typology adjusted to the sun exposure (p = 0.05, p = 0.001) but no findings were found for phosphatemia. Longitudinal studies should be initiated to consolidate our results and to raise awareness of the importance of diet for phosphocalcic status in Morocco.
I-Chuan Sheih
Ta Hwa University of Science and Technology, Taiwan.
Title: The antioxidant properties of a new antioxidative peptide (VG5)in different oxidation systems

Biography:
I-Chuan Sheih has ever been as a RD for four years in the Resource Microbiology Institute and a researcher for six years in Food Industry Research Development Institute. She has completed her PhD in 2009 and done a postdoctoral research for two and a half years in National Chung Hsing University. She is the associate professor of department of Food and Beverage Management in Ta Hwa University of Science and Technology and a project director of Ministry of Science and Technology. She has published many SCI papers in reputed journals at the same time.
Abstract:
In order to enhance the physiological effectiveness of the peptide used as medicine and health food in the future, we modified the original skeleton of natural multifunctional algae peptides (VECYGPNRPQF,VF11) for various antioxidant capacity detection, and utilized the bioinformatics knowledge to explore the relationship of amino acids diversity. The ABTS radical cation decolorization assay, was widely applied to evaluate the total antioxidative activity in both lipophilic and hydrophilic samples. The ABTS radicals scavenging activity of VECYG (VG5) is close to that of VF11 by hydrogen or electron donation, and it was far superior to YGPNRP (YP6) and PNRPQF (PF6). On the other hand, the penta-peptide VG5 (IC50 10.17 ± 0.12ïM) was also more close to those of f VF11 (IC50 9.44±0.35 ïM) and natural antioxidant Trolox( IC50 5.63±0.25 ïM) for quenching hydroxyl radicals capacity; and it was far superior to YP6 (IC50 56.41±0.23 ïM) and PF6 (IC50 148.37±1.46 ïM). We also further detected the oxidation-induced DNA damage to elucidate the prospective antioxidant role of peptide in human. The super coil (SC) form in DNA was completely converted to the open circular (OC) form due to the Fenton reaction (Fe2+ + H2O2) in our study. The result showed these peptides had the protective capacity in oxidation-induced DNA damage, so it further strengthened the ability of the peptides to protect hydroxyl radical induced damage. These results suggested the shorter chain peptide VG5 might be able to substitute for hendeca-peptide (VF11) to serve as food additives with health claims in the future.
Ngan T.K. Nguyen
Taipei Medical University, Taiwan.
Title: Predictors of type 2 diabetic remission of obesity individuals undergoing laparoscopic sleeve gastrectomy

Biography:
Ngan T.K. Nguyen completed her medical doctor at 26 years old, then became junior lecturer in her medical university, namely University of Medicine and Pharmacy in Ho Chi Minh city, Vietnam. Currently, she is a graduate student in the International Master Program in Medicine, College of Medicine, Taipei Medical University, Taiwan, supported by Scholarship of Ministry of Science and Technology(MOST), Taiwan. She is really interested in obesity and clinical nutrition research. She has published two papers in Vietnamese jounal after graduating medical degree and looked for more papers. She hopes stuyding futher in obesity and metabolism as Doctor of Philosophy.
Abstract:
Obesity and type 2 diabetes mellitus(T2DM) have become increasingly pandemic diseases in the world. By 2035, diabetes is expected to be mainly contributable in Asian populations who develop diabetes at younger ages than Western counterparts. The conservative therapies could not gain achievement in morbid obesity-induced T2DM compared to the metabolic/bariatric surgeries. Laparoscopic sleeve gastrectomy(LSG) has been increasing in Asia due to its simplicity and efficiency in weight management. Although weight loss is considered as a sign to improve insulin resistance in the diabetes, diabetic remission resulting from metabolic surgery for the obesity-T2DM patients who underwent LSG is still controversial. The optimal outcomes depend on selected markers from preoperative factors that are referred as prognostic values. The objective of this study is to investigate the diabetic remission and the possible preoperative diabetic predictors by using the latest Standards of Medical Care in Diabetes-2017 of American Diabetes Association. Materials and methods: We selected obesity patients who acquired T2DM and underwent LSG within 12 months. The complete diabetic remission was defined as glycated hemoglobin(HbA1c) <5.7% or fasting blood glucose<100mg/dL within one year without medication therapy or ongoing procedures. Changes of body composition and biomarkers were evaluated as preoperative predictors. Results: Of 129 T2DM patients who underwent LSG with mean body max index 41.3±6.8kg/m2. After 12-month follow-up, there were 59.7% (57/94) in the complete diabetic remission. Conclusion: It is expected that waist circumference, C-peptide and C-reactive protein involved in diabetic remission, which further suggest the diabetic remission of LSG in obesity-diabetes patients.
Shyh-Hsiang Lin
Taipe Medical University, Taiwan
Title: The neuroprotective effect of ketogenic diet on mice fed high-fat high-cholesterol (HFHC) diet

Biography:
Shyh-Hsiang Lin has been one of the faculty members in the School of Nutrition and Health Sciences in Taipe Medical University for 17 years. He has 15 publications in recent 4 years. His major research focuses on the relationship between nutritional factors and neurodegeneration.
Abstract:
Until 2012, there are more than 190,000 people who are suffering from the dementia. The morbidity is about 8% among those over 65 years old. AD, the most common form of dementia, is also known as the type3DM, which is highly correlated to abnormal regulation of insulin and glucose in the brain. Diets high in fat and cholesterol may have negative effects on health. Hyperlipidemia is considered one of the factors causing insulin resistance. On the other hand, hypercholesterolemia can cause BBB damage and promote the production of beta-amyloid (Ab) as well as increasing in oxidative stress. Ketone bodies are antioxidative and an alternative source of energy when glucose is no longer an energy source, which is useful under the condition of insulin resistance. In this study, a high-fat/high-cholesterol (HFHC) diet is given to mice for 16 weeks to induce insulin resistance and BBB damage, followed by feeding the mice a ketogenic diet or an HFHC diet with Metformin. We expect to observe that after receiving the ketogenic diet, the cognitive ability and the expression of AD-related proteins are regulated, in which the mechanism regarding the effect of a ketogenic diet will be clarified
Amit Bahukhandi
G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand (India)
Title: Variation in polyphenolics and Antioxidant Activity of Traditional Apple Cultivars from West Himalaya, Uttarakhand

Biography:
Mr. Amit Bahukhandi has submitted his Ph. D. degree from Uttarakhand Technical University, Dehradun, Uttarakhand (India) entitled “Altitude and habitat dependent variation in morphological and phytochemical diversity of selected Apple (Malus domestica Borkh.) cultivars in Uttarakhand”. During his Ph. D., his involvement was largely focused on quantification of secondary metabolites of different apple cultivars and investigated that how environmental and edaphic factors play role in secondary metabolites accumulation. Based on this work he has received “Young Scientist Award” in the 10th Uttarakhand Science Congress, Dehradun in 2016. In addition, he also worked on the chemical characterization and antioxidant activity of some threatened medicinal plants of Himalaya. Mr. Bahukhandi has published over 14 research paper in different National and International Journals of repute in addition to several popular articles and many conference presentations. Presently, he is focusing in “Forest Resource and Plant Biodiversity” under the National Mission For Sustaining The Himalayan Ecosystem at G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand (India).
Abstract:
Fruits are reported rich source of health benefit compounds, such as polyphenols and antioxidants. Regular consumption of fruits is known to reduce the risk of several degenerative diseases including cardiovascular and cancer. Polyphenols, which naturally present in fresh apples (Malus x domestica), are widely acknowledged as an antioxidant. However, the biochemical attributes of the cultivars of apple from west Himalaya are not systematically explored. In the present study, attempts were made to quantify the polyphenolic content and antioxidant activities in fully ripened fruits of 3 traditional apple cultivars namely Benoni, Fanny and Rymer growing at different locations of Uttarakhand (west Himalaya), India. Results revealed that phenolic (0.94-7.00 mg GAE/g fw), flavonoid (1.02-9.86 mg QE/g fw), flavonol (0.77-6.92 mg CE/g fw), tannin (15.49-37.99 mg TAE/g fw) and antioxidant activity (ABTS- 0.31-6.74; DPPH-0.50-1.41; FRAP-0.77-3.92 mM AAE/100g fw) varied significantly among the cultivars and the maximum content was recorded in Rymer fruits of Mukhwa location as compared to others. Altitude showed significant (p<0.05) positive relationship with total phenolic (r=0.895), flavonoid (r=0.843), flavonol (r=0.812), ABTS (r=0.856) and FRAP activity (r=0.830). Based on the results, it is concluded that Rymer is one of the most promising source of polyphenolic content and antioxidant activity and therefore, recommended for mass plantation at suitable localities for harnessing maximum potential.
Gayatri Mishra
IIT, Kharagpur, India.
Title: Detection of Rhyzopertha dominica Infestation in Stored Wheat Grain Using Near Infrared Hyper-spectral Imaging

Biography:
Gayatri Mishra has completed her Masters in Food Processing Technology in Anand Agricultural University, Anand, India.
Abstract:
Insect infestation in stored wheat grain is a potential hazard to the consumers and also fetching high economical loss to the farmers and food industries. Rhyzopertha dominica (lesser grain borer) is one of the most common insect grows over stored wheat grain causes major quantitative loss by feeding on the endosperm and also contaminate the grain by releasing excreta. High demand for zero contamination in food grain leads to advance analytical techniques for detection of insect infestation with high accuracy. Hyperspectral transmission images were acquired from normal and insect-damaged wheat grain over the spectral region between 400 nm and 1000 nm for 100 kernels. Ten statistical image features maximum, minimum, mean, median, standard deviation, and variance) and 10 histogram features were extracted from images. The statistical discriminant classifier namely support vector machine (SVM) was used to detect lesser grain borer infestation. Principal component analysis was used for wavelength selection; two wavelengths 900nm and 1000 nm corresponding to highest factor loading were found to be most significant for classification. SVM analysis correctly classified 93% of healthy and insect infested kernels at 1000nm wavelength region. The study demonstrated hyperspectral imaging can efficiently detect the insect infestation in stored wheat grain and can be used in food industries for rapid and online detection of stored wheat defects.
- Dear Nutrition 2018 attendees!
We would like to inform you that 3rd Day of the conference i.e., (May 16th, 2018) is scheduled for Business to Business Meeting, Networking with Keynotes, speakers, moderator and lunch will be provided from 12:30 -13:30.
We are very much glad to anticipate your presentation at networking and lunch session on 3rd day of the conference.
Thanks and regards,
Krishnam Raju
Nutrition 2018