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I-Chuan Sheih

I-Chuan Sheih

Ta Hwa University of Science and Technology, Taiwan.

Title: The antioxidant properties of a new antioxidative peptide （VG5）in different oxidation systems

Biography

Biography: I-Chuan Sheih

Abstract

In order to enhance the physiological effectiveness of the peptide used as medicine and health food in the future, we modified the original skeleton of natural multifunctional algae　peptides (VECYGPNRPQF,VF11) for various antioxidant capacity detection, and utilized the bioinformatics knowledge to explore the relationship of amino acids diversity. The ABTS radical cation decolorization assay, was widely applied to evaluate the total antioxidative activity in both lipophilic and hydrophilic samples. The ABTS radicals scavenging activity of VECYG (VG5) is close to that of VF11 by hydrogen or electron donation, and it was far superior to YGPNRP (YP6) and PNRPQF (PF6). On the other hand, the penta-peptide VG5 (IC50 10.17 ± 0.12M) was also more close to those of f VF11 (IC50 9.44±0.35 M) and natural antioxidant Trolox( IC50 5.63±0.25 M) for quenching hydroxyl radicals capacity; and it was far superior to YP6 (IC50 56.41±0.23 M) and PF6 (IC50 148.37±1.46 M). We also further detected the oxidation-induced DNA damage to elucidate the prospective antioxidant role of peptide in human. The super coil (SC) form in DNA was completely converted to the open circular (OC) form due to the Fenton reaction (Fe2+ + H2O2) in our study. The result showed these peptides had the protective capacity in oxidation-induced DNA damage, so it further strengthened the ability of the peptides to protect hydroxyl radical induced damage. These results suggested the shorter chain peptide VG5 might be able to substitute for hendeca-peptide (VF11) to serve as food additives with health claims in the future.