Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 10th World Congress on Nutrition & Food Sciences Osaka, Japan.

Day 2 :

  • Human Nutrition & Dietetics | Nutrition, Health & Aging | Dietary Supplements & Functional Foods | Probiotics & Prebiotics | Nutraceuticals & Medicinal Foods | Food & Nutritional Immunology

Session Introduction

Hiromi Murakami

Osaka Municipal Technical Research Institute, Japan

Title: Microbial Conversion of Lactose to Calcium Lactobionate
Speaker
Biography:

I have been working at Osaka Municipal Technical Research Institute since graduating from KONAN University in Kobe, Japan, and have been engaged in research into microbial enzymes, such as glycosidases, glycosyl transferases, oxidases. I received a doctoral degree  on “Levan degrading enzymes”. I have also been interested in enzymatic and microbioal oxidation of saccharides. Productions of Lactobionic acid, D-glucuronic acid and D-glucaric acid are good  examples for effective conversion of aldose to aldonic acid, uronic acid and aldaric acid.

Abstract:

Objective:  Concerned about utilization of lactose and development of high-soluble calcium supplement, with an interest on biological oxidation of oligosaccharide, we examined microbial and enzymatic oxidation of lactose and aimed to establish  effective production systems of calcium lactobionate. Lactobionate, b-1,4-D-galactosyl-D-gluconate, is an oxidized product of lactose. It has been reported to have mineral-absorption-promoting effects, Biffidobacterium-growing activity, moisturizing effect, and high solubility in water. Despite its useful properties, lactobionate has been supplied only in a small scale by chemical oxidation because there is no easy and efficient way to produce it.

Results:  As for fermentation, we isolated a mutant strain of Burkholderia cepacia which has no b-galactosidase activity to avoid hydrolysis of lactose and has sugar-tolerance to react with concentrated lactose. After 4-day cultivation, lactose was completely disappeared and the equivalent molarity of calcium lactobionate was accumulated. In the case of 10-day-fed-batch culture, the final concentration of the product reached 400 g/L in 100% yield. The product was purified from culture supernatant by ethanol precipitation.

 As for microbial conversion, cells were incubated with 100 to 200 g/L of lactose and half mole equivalent calcium carbonate to lactose. The oxidation activity of the cells was defined as the amount of cells which produced 1 mmol of D-gluconate per minute from 0.1M D-glucose under the assay conditions. When 2 U/mL of cells were incubated with 100 , 150 and 200 g/Lof lactose, it took 18, 27 and 48 h for 100% conversion. Reuse of resting cells was available for repeated conversions. Cells of Gluconobacter sp.were also used for microbial conversion. As for enzymatic conversion, we isolated a strain of Paraconiothyrium sp. which secreted a stable oxidase in culture. These biological conversion systems of lactose were effective to produce calcium lactobionate with high yield, no by-product, easy purification, and easy operation in one-pot synthesis.

Speaker
Biography:

Fatemeh Miremadi is an accredited practicing dietitian, specialized in hypercholesterlomia and food allergies, and working in different multidisciplinary medical centres across Melbourne, Australia since 2011. In 2013, she was awarded an Australian postgraduate award to commence her PhD at Victoria University, Melbourne. Australia.  Her project evaluates the combined effectiveness of probiotics, prebiotics and polyphenols in the synbiotic yogurt in modulating total cholesterol and LDL-C levels in mildly to moderately hypercholesterolemic population and its effectiveness in ameliorating cardiovascular disease risk factors in both women and men. She has already published 3 research papers leading journals and presenting her findings at national and international conferences

Abstract:

Statement of the Problem: The World Health Organization estimates 17.5 million deaths every year from cardiovascular diseases, particularly heart attacks and strokes. A substantial number of these fatalities can be attributed to lipid profile abnormalities, which triples the risk of heart attack in people with hypercholesterolemia, compared to those with normal blood lipid profiles. Various dietary approaches have been employed to alleviate hypercholesterolemia at the population level including the use of probiotics and prebiotics in development of functional foods. The purpose of this study is to evaluate the effects of a synbiotic yogurt, a novel product on lipid profile and blood pressure in mildly to moderately hypercholesterolemic and hypertensive subjects.

 Methodology & Theoretical Orientation: Synbiotic yogurt     contained probiotics L. rhamnusis and L. acidophilus, 2% prebiotic fructooligosaccharide enriched inulin (FEI) and 20% pomegranate juice concentrate (PJC) was used as dietary supplement. This product had 90% probiotics survivability, 72% total phenolic compounds and 68% antioxidant activity over a 4-week period of refrigerated storage. To assess its health benefits, an 8-week parallel, double-blinded, randomized trial was conducted with 48 male and female volunteers, aged 30-65 years, consuming a daily serve of 200g yogurt. Subjects were assigned to 3 groups: a control; group 2 consumed the synbiotic yogurt without PJC, and group 3 consumed the same synbiotic yogurt containing 20% PJC. Fasting blood samples, 3d dietary records, anthropometric measurements and BP were collected at baseline, end of 4 and 8 weeks.

Results: Consumption of the synbiotic yogurt containing PJC resulted in 6% decrease in total cholesterol and 8.3% decrease in low-density lipoprotein cholesterol (LDL-C) levels compared with the controls. Systolic blood pressure was reduced by 3.70 mmHg and diastolic blood pressure by 2.33 mm Hg. No significant changes from the baseline were observed in triglycerides and HDL-C levels. Total cholesterol: HDL-C ratio and LDL-C: HDL-C ratio as atherogenic indices significantly decreased in group 3 that consumed synbiotic yogurt containing PJC compared with the control group.

Conclusion: This study indicates the combined effectiveness of probiotics, prebiotics and polyphenols in ameliorating cardiovascular disease risk factors in both women and men

Speaker
Biography:

Abstract:

The Philippines is one of the world’s emerging diabetes hotspots. According to the International Diabetes Federation, the Philippines ranked in the top 15 in the world for diabetes prevalence.  Philippines is home to more than 4 million people diagnosed with the disease – and a worryingly large unknown number who are unaware they have diabetes. How a diabetic takes care of his health everyday determines the quality of his existence. The major purpose of the study was to determine the self-management needs of the diabetics to come up with a sound development program that could be implemented by the extensionists in the university, the health-care providers and civic-society organizations.  The respondents included all the diabetic patients registered in the Diabetes clinic of a government hospital.  Data were gathered through interview schedule.  Other pertinent data were taken from the patients’ individual medical chart.  Descriptive and inferential statistics were utilized to interpret the data.  Results showed that the areas of concern needed by the diabetics are meal planning, self-monitoring of blood sugar, sick day management, exercise, knowledge of high and low blood sugar and foot care.  The diabetics expressed their interest to become “managers” in their own care.

Speaker
Biography:

Abstract:

In the study was to investigate yogurts containing fish essential oil (EO) with sensory characteristics. Yogurt samples were categorised into five groups: C=control (with direct vat set starter culture), C1= 1% (sample with treated essential oil fish), C2=2% (sample with treated essential oil fish), C3=3% (sample with treated essential oil fish) and C4=4% (sample with treated essential oil fish) and stored at 4°C during 21 days. Samples were evaluated as color and appearance, texture, syneresis, flavor, acidity and general acceptability by 20 experienced panelists on the 1st, 7th, 14th and 21st days of storage. Of the different treated yogurt, both C1 and C2 yoghurt sample groups received higher scores in terms of total sensory characteristics (p<0.05). Panelists were thought to give low scores to overall flavor for the samples containing C4 by indicating a fishy taste and compared to other samples, C4 yoghurt samples were less preferred. It is a good way to elaborate food types by adding fish essential oil (EO) in them to improve their nutritional and physiological properties by regulating the rheological properties of the final product.

Yu-Kuo Chen

National Pingtung University of Science and Technology, Taiwan

Title: Pharmacokinetic Studies of pterostilbene and 3'-hydroxypterostilbene in rats
Speaker
Biography:

Yu-Kuo Chen received his Ph.D. in the Institute of Food Science and Technology from National Taiwan University in 2009. After completing around two years as a postdoctoral associate in the Department of Chemical Biology at Rutgers University, he joined the faculty at National Pingtung University of Science and Technology as an assistant professor in the Department of Food Science. Dr. Chen’s research focuses on the biological evaluation of functional foods, purification and identification of active components in functional foods, and analysis of metabolism and bioavailability of those compounds

Abstract:

Our previous results demonstrated that 3'-hydroxypterostilbene (OHPt) has higher anti-tumor activity than pterostilbene (Pt) in vivo. In the present study, we would like to elucidate whether the stronger biological activity of OHPt is due to its higher bioavailability than Pt. We performed pharmacokinetic studies in male Sprague Dawley rats. The animals received a single oral administration of OHPt and Pt at the dose of 50 mg/kg through oral gavage. After oral administration, a series of plasma samples were collected at 0, 5, 10, 15, 30, 60, 120, 180, 240, 480 and 600 min for further HPLC analysis. The results showed that two unidentified metabolites without parent compound OHPt were observed following ingestion of OHPt; while only one metabolite (glucuronide/sulfate conjugates) and parent compound Pt were detected in rat plasma after ingestion of Pt. These results indicated that the biotransformation of OHPt might not be glucuronide or sulfate conjugation, whereas, the major biotransformation pathway of Pt might be glucuronide or sulfate conjugation. The findings of this study provide message that despite structural similarity, Pt and its hydroxyl analog OHPt exert completely differential effects on oral bioavailability and metabolism. Further experiment is needed to identify the unknown metabolites of OHPt

Speaker
Biography:

Aysun Adan has her expertise in Molecular Biology of Cancer and Signaling Pathways, Natural Plant Products and Their Anti-carcinogenic potentials, Multidrug Resistance Mechanisms and Reversal of Resistance, Apoptosis, The Involvement of Bioactive Sphingolipids in Apoptosis and Drug Resistance, Combination Therapies, Stem Cells and Their Regenerative Potential

Abstract:

The studies elucidating the roles and the mechanisms of action of fisetin and hesperetin, plant flavonoids, in acute promyelocytic leukemia (APL) and chronic myeloid leukemia (CML) are absent. In this study, we investigated the mechanism of the antiproliferative and apoptotic actions exerted by fisetin and hesperetin on human HL60 APL and human K562 CML cells. The viability of HL60 and K562 cells was evaluated using the MTT assay, apoptosis by annexin V/propidium iodide (PI) staining and cell cycle distribution using flow cytometry, and changes in caspase-3 enzyme activity and mitochondrial transmembrane potential were determined. Moreover, we performed whole-genome microarray gene expression analysis to reveal genes and biological networks affected by KEGG and IPA analysis. For APL cells, both flavonoids showed a concentration- and time-dependent inhibition of proliferation and induced G2/M arrest and G0/G1 arrest for hesperetin at only the highest concentrations. There was a disruption of mitochondrial membrane potential together with increased caspase-3 activity. Furthermore, findcution of apoptosis was confirmed by annexin V/PI analysis. The microarray gene profiling analysis revealed some important biological pathways including MAPK and inhibitor of DNA binding (ID) signaling pathways altered by fisetin and hesperetin treatment as well as gave a list of genes modulated involved in cell proliferation, cell division, and apoptosis. For CML cells, fisetin and hesperetin inhibited cell proliferation and triggered programmed cell death. The latter was confırmed by mitochondrial membrane depolarization and an increase in caspase-3 activation. In addition to that, we have detected S and G2/M cell cycle arrests and G0/G1 arrest upon fisetin and hesperetin treatment, respectively. The microarray gene profiling analysis revealed some altered important signaling pathways including JAK/STAT pathway, KIT receptor signaling. They both significantly modulated the expression of genes involved in cell proliferation and division, apoptosis, cell cycle regulation, and other significant cellular processes such as replication, transcription, and translation. 

Patarapan Odton

University of the Thai Chamber of Commerce, Thailand

Title: Breastfeeding and Nutritional Status among Children in Thailand
Speaker
Biography:

Patarapan Odton has her Ph.D. in Research Methodology, Prince of Songkla University. She started working in health economics research at Ministry of Public Health since the year 2006. She’s moved to do research in economics of education during the past 5 years. She has expertise in health economics, statistical modelling, and GIS

Abstract:

Background: Breastfeeding is the gold standard when it comes to infant nutrition, and no artificially produced infant formula will ever be able to replace it. In the first years of life, breast milk protects infants from infections by passing on their mothers’ antibodies. In Thailand, there were many studies on prevalence and determinants of breastfeeding since National Breastfeeding Project began in 1989. Only few studies related to effects of breastfeeding on child’s nutritional status.

Objective of this study is to investigate impact of breastfeeding practices on nutritional status of children.

Methodology: The Thailand Multiple Indicator Cluster Survey (MICS3 and MICS4) are the main data source. For assessing the nutritional status of children, we use three anthropometric indices, weight-for-age, height-for-age, and weight-for-height. Regression models are performed to assess the effects of breastfeeding on child’s nutritional indices, including demographic and socio-economic factors.

Findings: Based on weight-for-age, height-forage, and weight-for-weight z-score, infant aged 0-5 months with exclusively and non-exclusively breastfed are not significantly different in all those three indices. During ages 6-11 months, infants who never been breastfed are moderately underweight and moderately stunted, much smaller and shorter than who ever been breastfed. There are no different in height-for-age when they get older, aged 24-59 months. Results from regression models indicate that infant with less than 6 months of breastfed are significantly higher in weight-for-age z-score than who never been breastfed. Children have been longer breastfed (24 months and over) are smaller than who never been breastfed. It’s clear that longer breastfed than 12 months can effects to lower weight-for-height.

Conclusion: Breastfeeding effect to nutrition indices when infant aged 6-11 months. Longer breastfed can effect to lower weight-for-age, height-for-age, and weight-for-height when they aged 12-47 months. At the fifth year of life, all children are in the standard weight and height.

Speaker
Biography:

Qian Li has her expertise in Food Nutrition and Health and just got her PhD in June 2016 from South China University of Technology, China. Her research program has been engaged in the scientific research on the structure identification and activity evaluation of natural products, polysaccharides and phenols. She have one year of study and research experience in Bioactive Natural Products and Phytoceuticals (BNPP) Lab of Michigan State University, College of Agricultural Natural Resources. She has published 9 articles in first author and 7 of them are high qualitied SCI. She has her expertise in the extraction, isolation, structure identification and structure-activity relationship of natural product. Her research program addresses all aspects of sericulture resources and Characteristic Lingnan fruits/vegetables nutrition, bioactive substances and the related functional evaluation since she got her job from Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, China.

Abstract:

Chinese yam polysaccharides were isolated using the method of boiled water extraction and stepwise ethanolic precipitation, combined with the tangential flow ultrafiltration membrane system. Their molecular weights were determined by high performance gel permeation chromatography. Three type yam polysaccharides in different molecular weight were isolated: HSY-I (> 50 kDa), HSY-II (10 ~ 50 kDa), HSY-III (< 10 kDa). The monosaccharide and glycosidic bond links composition were analyzed with GC and Smith degradation. The structure characteristics were further discussed combined with infrared spectrophotometry. Dexamethasone-induced insulin resistance glucose/ lipid metabolism diabetic mice model was established to evaluate the hypoglycemic effect of different concentration of HSY and different molecular weights polysaccharide HSY-I, HSY-II, HSY-III. The results indicated that the HSY polysaccharide mixture, HSY-I and HSY-II had auxiliary hypoglycemic effect. 

Speaker
Biography:

Rany Adelina has her expertise in nutrition and health sciences. Her focus is to developing research in children and young adult nutrition She has finished her master in Nutrition and Health Sciences, Taipei Medical University, Taiwan  after years of experience in clinical and community nutrition research. Now, she is working as a faculty member for both teaching and doing research in Nutrition department, Malang health polytechnic, Malang city, Indonesia.

Abstract:

Statement of the Problem: The background of this study is referring to the phenomenon of Indonesian students who live on the outside from their hometown tend to have a careless eating habit. The difference in study location may cause a different type of eating habits between Indonesia and overseas, furthermore study intensity, meal preparation, and food access also may be influenced by this difference of study location. The purpose of this study is to determine the correlation between study location and eating habits for students who go abroad in Malang, Indonesia and in the city of Taipei, Taiwan.

Methodology & Theoretical Orientation:
Subjects were Indonesian college students who live on the outside from their hometown for doing the study to Malang city, Indonesia and Taipei city, Taiwan. The total target sample was obtained as many as 493 respondents (397 respondents in Malang city and 96 respondents in Taipei City). Measured variables were studied intensity, meal preparation, food access, and eating habits in both two cities.

Findings: The Indonesian college students who lived in both Malang city and Taipei city, did not have the significant difference in eating habits. However, the trend showed healthier eating habits in Malang city.  On the other hand, study intensity, meal preparation, and food access were significantly different in both cities. Indonesian college students in Taipei tended to have higher prevalence of low study intensity, dependent meal preparation, and difficult food access. 

Conclusion & Significance:
There is the significant correlation between study location and study intensity, meal preparation, food access, but not eating habits. Future research is needed to know the reasons for this insignificant correlation between eating habits in this two countries.

Speaker
Biography:

Qunyu Gao is an affiliate of South China University of Technology, China

Abstract:

Sweet potato starch was subjected to cross-linking to achieve high content of resistant starch (RS) under heat-moisture treatment and the physicochemical properties of cross-linked sweet potato starches were investigated. A maximum RS content (72.45%) was obtained after 10% STMP/STPP (99/1% w/w) at 120℃ and pH 11.5 with a 20% moisture level. Through the analysis of 31P nuclear magnetic resonance (31P NMR) spectroscopy, the RS-72.45% contained distarch monophosphate (DSMP), not  be detected monostarch monophosphate (MSMP). After cross-linking, the starches with high moisture treatment (≥25%) were observed loss of birefringence and surface erosion. The X-ray diffraction patterns showed unchanged in crystal type but with slightly alteration in the relative crystallinity of cross-linked starches. The gelatinization temperature increased as the moisture content increased, while gelatinization enthalpy apparently decreased. 

Yi-Zhen Chen

National Pingtung University of Science and Technology, Taiwan

Title: Regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters
Speaker
Biography:

Yi-Zhen Chen is a second year master student in the Department of Food Science at the University of National Pingtung University of Science and Technology. She obtained her bachelor's degree in Food Science at NPUST in June 2015. She's full of great interests in the knowledge about food science. For her Master’s Thesis subject is the regulation effect of Djulis hull on blood lipids in hyperlipidemic hamsters. She has published one peer-reviewed research article in SCI international journal and five conference posters, and she has passed the required qualification examination for six technician certificates.

Abstract:

Heart disease and cerebrovascular disease are the second and third leading cause of death in Taiwan in 2015. Many studies showed that the most important factor leading to cardiovascular-related diseases is atherosclerosis, and the main caused by atherosclerosis is high blood cholesterol levels. Over the years, the risk of cardiovascular disease associated with population continues to increase, so the healthy diet and exercise have become one of the important issues. Djulis (Chenopodium formosanum) is a native cereal plant in Taiwan, and the djulis hull (DH) was found to be its major source of antioxidant compound. Studies showed that it possesses several active components with hypolipidemic activities, such as dietary fiber and polyphenol compounds. The objective of this investigation was to evaluate the regulation effects of DH in blood lipid levels to achieve the prevention of atherosclerosis effect. Five weeks old hamsters were randomly divided into five groups: (1) control group, (2) hyperlipidemic group (high fat diet containing 0.2% cholesterol; HFC), (3) low-dose DH group (HFC diet and treated with 100 mg/kg/day DH; LDH), medium-dose DH group (HFC diet and treated with 250 mg/kg/day DH; MDH), high-dose DH group (HFC diet and treated with 500 mg/kg/day DH; HDH). After 8 weeks of feeding study, the results showed that levels of triglyceride (TG; LDH: 194±31 mg/dl; MDH: 194±25 mg/dl; HDH: 204±26 mg/dl), total cholesterol (TC; LDH: 328±25 mg/dl; MDH: 301±21 mg/dl; HDH: 283±41 mg/dl) and low-density lipoprotein cholesterol (LDL-C; LDH: 165±33 mg/dl; MDH: 145±31 mg/dl; HDH: 140±28 mg/dl) in serum were significantly reduced by DH administration compared with those of HFC group (TG: 309±64 mg/dl; TC: 398±79 mg/dl; LDL-C: 184±31 mg/dl). Moreover, levels of TG and TC in liver were lower than HFC group, and levels of TG and TC in feces were higher than HFC group. These results indicate that DH possesses hypolipidemic potential to be a benefit functional food to prevent high fat/high cholesterol diet-induced hyperlipidemia.

Speaker
Biography:

Said Hassan is a PhD Scholar at Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy,Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China. Prior to PhD studies Hassan has done B.Sc Hon and M.Sc Hon in Agriculture Chemistry. His main field of interest is natural products and published 15 research articles. 

Abstract:

Experimental based evidence suggests that most of the medicinal plants possess a wide-ranging pharmacological and biological activities. The present study was designed to evaluate the protective effect of methanolic extract of Monotheca buxifolia against carbon tetra chloride induced genotoxicity and DNA damages in liver tissues of rats. Adult albino rats of either sex were divided randomly into three different groups to assess the hepatoprotective protentional of Monotheca buxifolia against CCl4 induced genotoxicity, DNA damage and antioxidants reduction. Rats of control group I were provided access of water and food. Group II rats were given 1 ml/kg of CCl4 (30 % in olive oil v/v) via intraperitoneally twice in a week for 28 days. While group III was getting 200 mg/kg of b.w MB via oral route after 48 hours of CCl4 administration. Protective effect of MB was assessed by measuring the serum marker, assays of antioxidants enzymes, genotoxicity and DNA damages. The current study disclosed that treatments of MB invert the activities of serum markers enzymes and cholesterol profile as damaged by CCl4 treatments. Activities of antioxidants enzymes of the liver tissues homogenates; catalase (CAT), superoxide dismutase (SOD), per oxidase (POD) were reduced with CCl4 administration, which were retained with MB. CCl4 induced hepatic injuries increasing the lipid peroxidases product (TBARS), were controlled by treatments with MB. Furthermore, the administrations of CCl4 caused DNA fragmentation and genotoxicity which were significantly retained to normal level with MB. These investigation shows that treatment of Monotheca buxifolia may be helpful in the preventions of hepatic stress.

Speaker
Biography:

Jia Lin Guo study in national pingtung university of science and technology master’s degree program in food science, and he has expertise of HPLC analysis in natural compound.

Abstract:

Tangeretin (TAN), a kind of polymethoxyflavones (PMFs) found in citrus peels, has been proved to have many kinds of bioactivities including anti-inflammatory, anti-atherosclerosis and antitumor activities. Prostate cancer is universal malignant in male genital system. According to studies, 5-demethyltangeretin has hydroxyl group at the C5 position, and higher cytotoxicity than TAN for cancer cells, but the low solubility of 5-DTAN limits their availability in biological system. For the reason that we prepared 5-DTAN derivative (with acetyl group at the C5 position, 5-acetyltangeretin derivative; 5-ATAN), and hope to increase its solubility and bioavailability. The object of this study is to investigate the anti-proliferation and cellular uptake of 5-ATAN in PC-3 human prostate cancer cells. Esterase present in the cells, therefor, 5-ATAN will be converted to 5-DTAN when 5-ATAN was uptaken into cells. We could use high-performance liquid chromatography lined electrochemical detector (ECD) to detect concentration of 5-DTAN within PC-3 cell. In MTT assay, it demonstrated that 5-ATAN significantly inhibit growth of PC-3 cells in dose- and time-depend manners. In condition of HPLC-ECD showed that 300 mV is the best volt, and retention time of 5-DTAN is 11.6 minutes, and linear regression is 0.1-10 µM. Moreover, the quantification and detection limits (LOQ and LOQ) of 5-DTAN were 7.16 and 1.79 μg/ml, respectively. In bioavailability test, intracellular concentration of 5-DTAN of 5-ATAN-treated group was significantly higher than those of 5-DTAN-treated group, which showed dose- and time-depend responses. Overall, the results indicate that 5-ATAN showed highest anti-proliferation activity than TAN & 5-DTAN in PC-3 cancer cells, and had greater bioavailability than 5-DTAN. 5-ATAN will be expected to have better oral bioavailability in vivo.

Speaker
Biography:

Ms  Marietta M. Bumanglag, RND,  is a Senior Science Research Specialist at the FNRI-DOST, the country’s lead agency in food and nutrition research & development. She obtained her  Master of Public Health from the University of Queensland, Australia.  Her project involvement includes Recipe development, Recipe/menu evaluation and Development of dietary tools :  recipe booklets  and Menu Guide Calendars (MGC) since 2004 as senior author. Her publication included the FNRI MGCs, LUTONG FNRI: Brown Rice recipes para sa Lahing Kayumanggi and Mga Katutubong Gulay, Recipe booklet utilizing malunggay leaves powder and yacon tubers, and Nutritious recipes for the FNRI supplementary feeding program

Abstract:

Failure to meet increased demands for energy and nutrients and poor eating habits have long term health, growth and development consequences in adolescents. A calendar to promote consumption of healthier meals in adolescents to help meet their nutrient needs through consumption of legumes and nuts was developed. The following activities were conducted: Focus Group Discussion (FGD) among panelists (n=8) to explore adolescents’ perceptions and insights towards food, eating behavior and food preferences; two (2) recipe trials and sensory evaluation to determine acceptability of recipes based on the Hedonic Rating Scale scores of sensory panelists (n=15; n=50) ; estimation of energy and nutrient content of recipes using the FCT + Menu Eval Software; photo-documentation and formulation of 4-week cycle menus. Themed “Helping meet the nutrient needs of adolescents through consumption of legumes and nuts”, the MGC 2016 highlights 15 recipes consisting of snacks, one-dish meal and viands incorporated in the 4-week cycle menu. The recipes utilized locally available and familiar legumes: green and red mung beans, lima beans, peanuts, white and red kidney/snap beans, pigeon pea and string bean seeds. Other features included articles on legumes and nuts, nutritional needs of teenagers and tips on encouraging teens to eat healthy foods and Pinggang Pinoy for teens, 13-18 years. The calendar may serve as a reference/guide for homemakers/food providers in preparing nutritious and varied meals, and a challenge for adolescents to try new and exciting recipes. The MGC is downloadable in the FNRI website for wider audience reach and dissemination

Po-Hua Wu

National Pingtung University of Science and Technology, Taiwan

Title: Regulation effect of tempe (tempeh) in serum glucose in type II diabetic rats
Speaker
Biography:

He is Pu-Hua Wu, Taiwanese. He is a second year master student at Department of Food Science in National Pingtung University of Science and Technology. As its name, it is located at Pingtung, Taiwan, pure and simple counties. He was born and grown in Pingtung. So He has more emotion not only Pingtung but this school

Abstract:

In recent years, tempe (tempeh) is being more attention than before. Tempe (tempeh) originates from traditional food of Indonesia, is produced by termentation of soybean. Traditional tempeh only uses Rhizopus to ferment. Trying Lactobacillus co-culture with Rhizpous to create more comfortable condition for fermentation. Diabetes, an increasingly serious disease of modern civilization. A healthy body secretes insulin to regulate blood glucose by pancreas. Through the action of insulin so that glucose in the blood transport into cells to provide energy which cells need. When the level of insulin is not enough or inefficient use of insulin to reduce blood sugar in body, the problem of high blood sugar will occur. The most common diabete is type II, which is acquired diabetes. It is confirmed that aglycone can help regulation of blood sugar. Tempe has more aglycone than other soybeans product. This study observes changes of blood sugar in diabetic rats by fed tempe. To induce diabetes uses pharmacy with high-fat diet. Using streptozotocin (STZ) with nicotinamide induces rats to get diabetes and make sure their blood sugar above 150 (mg/dl). At same time, rats had fed high fat diet which include cholesterol, coconut oil and normal diet. Comparing tempe, tempe with Lactobacillus are useful with negative control and soybeans groups. Obviously, diabetic rats regulation of blood sugar with tempe are very useful.

Speaker
Biography:

Ghazi Daradkeh is an affiliate of Department of Dietetics, ALkhor Hospital, Hamad Medical Corporation, Qatar

Abstract:

Background: Prevalence of overweight and obesity among children and adolescence over the past three decades reached an alarm­ing point in both developed and developing countries. As a result of the economic boom overweight and obesity has become a major health problem in the Arabian Gulf countries particularly in Qatar. Breakfast is considered to be the first and most important meal of the day. Breakfast skipping may be one of the complexes and multi-factorial causes of obesity.

Aims: The aims of this study were (1) to examine the prevalence of breakfast skipping among adolescent in the state of Qatar. (2) Evaluate the hypothesis that breakfast skipping would be associated with increased overweight and obesity and central obesity in the study population.

Method: A cross-sectional study was carried out among 1225 adolescents (625 boys and 600 girls) aged 15 to 18 years were selected by means of a multistage stratified random sampling technique from 14 public and 7 private schools between October 2012 and February 2013. Anthropometric measurements: Weight in (kg), height in (cm), Body Mass Index (kg/m²), waist circumference (cm) and waist to height ratio (WHtR) were measured/computed. Demographic data and Food habits were collected by using structured questionnaire.

Breakfast eating pattern was classified as: Breakfast eaters (students who are ate breakfast at least 4 days in a week). Breakfast skippers (students who skipped breakfast at least 4 days in a week).

Results: Breakfast skipping is a common unhealthy habit, out of 1225 participants 766 (62.5%) consumed breakfast less than daily. 52.5% of the participants were breakfast skipper (ate breakfast less than 4 times/week). Weight, Body Mass Index (BMI), waist cir­cumference (WC), was significantly different between participants age in both gender p< 0.05. The prevalence of overweight (43.0%) and obesity (39.5%) was lower among breakfast eaters than skippers (56.7% & 60.5%) respectively. In addition central obesity (WHtR ≥0.5) was more prevalent among breakfast skippers than eaters (59.3% vs 40.7%) respectively. A dose-response relationship was shown to exist between breakfast consumption and being overweight and obese among all students. This study showed that breakfast skippers were heavier than eater in both genders; body mass index was significantly higher among breakfast skippers.

Conclusion: Regular breakfast consumption is negatively associated with overweight and obesity among adolescents in Qatar, we found that breakfast skipping was prevalent in our study sample, and was associated with overweight, obesity and central obesity in both gender. Awareness programs about the importance of breakfast meal are needed for adolescents

Chien-Hua Huang

National Pingtung University of Science and Technology, Taiwan

Title: Study on the protective effects of tocotrienols in SH-SY5Y cells
Speaker
Biography:

Chien-Hua Huang is a first year master student in the Food Science Department at National Pingtung University of Science and Technology. She has a great passion for food science and has the expertise in cell and animal experiments and biochemical techniques such as Western blotting. Chien-Hua Huang is studying the protective effect of Corbicula fluminea extracts in RGM-1 cell against indomethacin-induced gastric mucosal injury. She has published four posters, one of which was presented at the ISNFF International Conference in 2016

Abstract:

Tocotrienols and tocopherols, naturally occurring isoforms of vitamin E, are found abundantly in food such as palm oil, rice bran oil, corn, oats, barley, rye and wheat. Tocotrienols have four different isomers: α-, β-, γ- and δ-forms. The present study was designed to evaluate the protective effect of tocotrienols against methylglyoxal (MG)-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. In order to assess the protective effect of tocotrienols, α-tocotrienol (α-T3), γ-tocotrienol (γ-T3) and δ-tocotrienol (δ-T3) were administered to SH-SY5Y cells for 2 hours prior to the induction of cytotoxicity by MG, and the cell viabilities of SH-SY5Y cells were analyzed by MTT assay. The cell viability of SH-SY5Y cells were reduced to 40% by 24 h treatment of 0.5 mM MG, and two hours pretreatment of α-T3, γ-T3 and δ-T3 increased the cell viability to 75%, 53% and 82%, respectively. It is shown that T3 has protective effect on the neuro-toxicity induction by MG. Among T3, δ-T3 has the best protective activity. The future work needs to be performed to elucidate the neuro-protective mechanism of T3.

Speaker
Biography:

Abstract:

HPLC-fluorescence detection;LC-MS-MS

The identification and analysis of three anti-oxidants including: trans-resveratrol, quercetin and emodin in grape and red wine have been studied by HPLC-fluorescence and LC-MS/MS detection with SFE in this work.

Trans-resveratrol (trans-3,5,4'-trihydroxystilbene,resveratrol) is common found in grape and red wine. Trans-resveratrol (trans-RSV) has important effects on lipid metabolism and can be used as anti-inflammatory, anti-oxidant, free radical scavenging, and anti-allergy. It can also prevent the cardiovascular diseases. Besides, trans-RSV is also an effective cancer preventive medication. It can effectively inhibit tumor growth and cancer formation. Trans-resveratrol is sensitive to light and heat. Consequently, trans-RSV will convert to inactive cis-RSV.  The coexistence of antioxidants will also cause activity recession.

In this work, quercetin (2-(3,4-dihydroxyphenyl)- 3,5,7-trihydroxy-4H-chromen-4-one) and emodin (1,3,8-trihydroxy-6-methylanthracene- 9,10-dione are also studied for their interferences in the analytical procedure for trans-RSV. The sensitivity and recovery for the proposed method for natural trans- resveratrol from grape and red wine are also evaluated.

Speaker
Biography:

Ching-Yi Kuan is a first year master student in the department of Food Science of National Pingtung University of Science and Technology in Taiwan. She has a great passion for food science. She can do cell experiments, Western blotting and animal experiments. She is studying the inhibitory activity of the stilbenes on liver cancer. She has published one poster in Taiwan Association for Food and Technology in 2015

Abstract:

According to the Ministry of Health and Welfare in Taiwan, prostate cancer is the sixth highest cause of cancer-related   death. Besides, prostate cancer cells will metastasize to bones and lymph nodes, so it’s important that prevention and treatment of prostate cancer. In this study, 3'-hydroxypterostilbene (OHPt), a structural analogue of pterostilbene (Pt), were used as experimental samples to investigate the potent anti-tumor effects in PC-3 xenografted nude mice. 1×106 PC-3 cells were injected subcutaneously into the flanks of 30 male Balb/c nude mice to form tumors. When xenografted tumor volume reached around 100 mm3, mice were randomly divided into five groups. Each group consisted of 6 animals. Mice were orally administrated with daily doses of OHPt (5, 25 and 50 mg/kg/d) and Pt (25 mg/kg/d) for 4 weeks; while control group was orally administrated with olive oil only. During the experiment, the tumor volume was determined and recorded once or twice per week using caliper measurements. At the end of the experiment, animals were sacrificed by CO2 asphyxiation and the individual tumors were excised immediately and weighed. Further the protein expression of tumors was measured. The results showed that the treatment of mice with OHPt could inhibit the tumor volume and tumor weight in a dose-dependent manner, and increased the expression of c-caspase-3 while decreased the expression of MMP-9 and Cox-2. In conclusion, these results revealed that OHPt might be a potential agent to treat prostate cancer.

Speaker
Biography:

Diana Mareta IFA Pitaloka, graduated from nutrition and health of Brawijaya University, is a master student of Taipei Medical University (TMU), Taiwan. She got basic knowledge about clinical nutrition, food science, and community nutrition at bachelor degree. From three of them, she was interested in clinical nutrition and food science excellently.At the master program, she concern about high fat diet, inflammation, and lipid metabolism. Recently, she learn about glutamine and arginine against lipid metabolism in the liver.

Abstract:

Introduction: Status epilepticus is a condition which has seizures more than 30 minutes without recovery between them. Seizures in the meningoencephalitis patients are caused by enhancement of emphasis and expansive of inflammation area in the brain. The ketogenic diet was given to the epileptic patient to minimize the seizures through some pathways such as: inhibit neurotransmitter release and activation of ATP-sensitive potassium channels, enhance overall bioenergetics and reduce oxidative stress, and modulate tricarboxylic acid flux.

Objection: The aim of this case study is to know the effectiveness of ketogenic diet in order to decrease seizures in a patient with status epilepticus. Method: This study was a case study which observed a patient (boy, 10 years old) at the Dr. Saiful Anwar Hospital, Indonesia, with status epilepticus and loss of consciousness. The ketogenic diet was given gradually, for 7 days (February 25th- March 3th, 2015), a patient was given pan enteral diet 1200 cc/day    (1200 kcal, protein 36.72 g, fat 61,8 g, and carbohydrate 130.8 g), for 9 days (4th-12th March  2015) received ketogenic diet 1600 cc  (2169.9 kcal,   protein 16.32 g, fat 107.2 g, and carbohydrate 280 g).

Result: Seizure decreased at the first day after ketogenic diet administration (seizures <30 minutes) and stop completely at second day after administration. Based on monitoring result, a patient had decreasing body weight and MUAC during ketogenic diet administration. Furthermore, patient had enhancement of lipid profile (total cholesterol 211 mg/dL, TG 256 mg/dL, HDL 32 mg/dL, dan LDL 146 mg/dL).

Conclusion: The ketogenic diet which given gradually can reduce and inhibit seizures in the meningoencephalitis patient.

Taejoon Kang

Korea Research Institute of Bioscience & Biotechnology, South Korea

Title: A novel and highly specific phage endolysin cell wall binding domain for detection of Bacillus cereus
Speaker
Biography:

Taejoon Kang received a B.S. in chemistry from KAIST in 2004 and a Ph.D. in chemistry from KAIST in 2010. He did postdoctoral research at KAIST before joining in KRIBB as a senior researcher in 2012. He is currently senior researcher of BioNanotechnology Research Center and BioNano Health Guard Research Center in KRIBB and Associate Professor in major of Nanobiotechnology and Bioinformatics at UST

Abstract:

Rapid, specific and sensitive detection of pathogenic bacteria is crucial for public health and safety. Bacillus cereus is harmful as it causes foodborne illness and a number of systemic and local infections. We report a novel phage endolysin cell wall-binding domain (CBD) for B. cereus and the development of a highly specific and sensitive surface plasmon resonance (SPR)-based B. cereus detection method using the CBD. The newly discovered CBD from endolysin of PBC1, a B. cereus-specific bacteriophage, provides high specificity and binding capacity to B. cereus. By using the CBD-modified SPR chips, B. cereus can be detected at the range of 105–108 CFU/ml. More importantly, the detection limit can be improved to 102 CFU/ml by using a subtractive inhibition assay based on the pre-incubation of B. cereus and CBDs, removal of CBD-bound B. cereus, and SPR detection of the unbound CBDs. The present study suggests that the small and genetically engineered CBDs can be promising biological probes for B. cereus. We anticipate that the CBD based SPR-sensing methods will be useful for the sensitive, selective, and rapid detection of B. cereus.

Ponpis Raungkhajon

Prince of Songklanagarin Hospital, Thailand

Title: Nutritional management in Upper gastrointestinal tract cancers
Speaker
Biography:

Abstract:

In a study of 224 patients, comprising 157 male patients and 57 female patients, to determine the correlations between Body Mass Index (BMI) and the risk of malnutrition, 124 patients were found to have BMI of 16.00-18.49 Kg/m2 while 54 patients were found to have BMI less than 16.00 Kg/m2. Using the same sample, an assessment of nutritional status of the patients using the Nutrition Alert Form shows that 78 patients had severe malnutrition while 112 patients had moderate malnutrition. In addition, by monitoring the change in patients’ weight over the course of 4 weeks, the study shows that 69 patients or 30 percent of the total sample experienced over 5% weight-loss, 36 patients or 16 percent experienced 2 - 5% weight-loss, and 108 patients or 48.2 percent experienced 1 – 2 % weight-loss. 160 patients had low Albumin serum with 143 patients having 2.0 - 3.5 mg% of Albumin serum and 17 patients having less than 2.0 mg% of Albumin serum.

Patients received Enteral Nutrition Support according to Nutrition Care Process for Cancer Treatment Plan with 115 patients or 51.3 per cent of the total sample receiving 1.2 kcal/1kd/d and 15-20 gm protein, 66 patients or 29.4 per cent receiving 1.25 kcal/1kd/d and 10 - 15 gm protein, 31 patients or 13.8 per cent receiving 1.5 kcal/1kd/d, and 12 patients or 5.5 per cent receiving 1 kcal/1kd/d and 20 - 40 gm protein. Our patient care team is a multidisciplinary patient care approach designed to implement a mixed and integrated patient care system. In particular, the pre-treatment nutritional care is central to the reduction of risk of malnutrition and allows quick and uninterrupted access to nutritional care through our Care Process.

Speaker
Biography:

Chien is an assistant professor of food science, nutrition, and nutraceutical biotechnology at Shih Chien university in Taiwan. His research field included food analysis and bioassay especially focus on anti-cancer, anti-inflammatory, and analgesic effects by phytochemicals from food (Chien & Wang, 2016). Recently, he developing the anti-aging model to elucidated the protective effects of medical food on degenerative diseases such as osteoarthritis, sarcopenia, and neurodegeneration

Abstract:

As the world aging rapidly, neurodegenerative diseases became one of the most serious age-related health problems. In south Asia, several medical foods have been used as an anti-aging remedy since ancient times. Cordyceps (Paecilomyces hepiali mycelium), a traditional tonic, has been reported with various pharmacological activities. In this study, we utilized Drosophila model system mimicking Huntington’s disease (HD) to exam the neuroprotective effects of the ethanol extraction of P. hepiali mycelium (PHE). These HD flies are expressing motor dysfunction and early death. Results demonstrated that diet containing 1 mg/mL PHE could significantly prolong the mean lifespan up to 31% of flies by compared to the control diet. It was also found that PHE could improve the locomotor activity of flies at the young age (4 days). Besides, oxidative stress plays a causal role in the neurodegenerative process. PHE exhibit antioxidative properties in a concentration-dependent manner by using the DPPH radical scavenging and ferric ions reducing power assay. These results indicated that supplementation with PHE improved movement performance and longevity might contribute by its neuroprotective antioxidants. We suggest that the PHE can be developed as a potential dietary supplement against neurodegenerative diseases such as HD

Pakjira BenyaPanya

Prince of Songklanagarin Hospital, Thailand

Title: Developing soy and soy-based recipes for menopausal women
Speaker
Biography:

Abstract:

Objectives: To study and develop recipes from soy and soy-based products for female patients of OBGYN clinic at the Prince of Songkhla University Hospital who are in their menopause

Methodology: Developing 4 recipes from soy and soy-based products

Result: Southern Coconut-Rice with Chicken Curry provides the highest level of energy at 460 Kcal, followed by Stuffed Glutinous Rice Balls at 335 Kcal, Spicy Tofu at 270 Kcal, while Baby Mushroom provides the lowest level of energy at 190 Kcal. In addition, a look at how patients assess the recipes shows that the effectiveness of the recipe in controlling underlying medical conditions ranks highest with an average score of 4.43, followed by taste at 4.31, and presentation/appeal at 4.17. The assessment criteria with the lowest score was the ease of cooking, which only received 3.95 on average. The recipes were then assessed on 3 subsequent assessment criteria with the following results:

Taste: Stuffed Glutinous Rice Balls scored highest at 4.6, followed by Spicy Tofu at 4.3, and Coconut-Rice with Chicken Curry at 4.25. Baby Mushroom scored lowest at 4.1.

Presentation/Appeal: Spicy Tofu scored highest at 4.47, followed by Stuffed Glutinous Rice Balls at 4.2, while Coconut-Rice with Chicken Curry and Baby Mushroom received an equal score of 4.

Ease of Cooking: Spicy Tofu score highest at 4.2, followed by Coconut-Rice with Chicken Curry at 4, while Baby Mushroom and Stuffed Glutinous Rice Balls received an equal score of 3.8.

Speaker
Biography:

Liezl M. Atienza has her expertise in human nutrition. She is a registered nutritionist-dietitian and an Asst. Professor in the University of the Philippines Los Banos. Her current researches are focused on lifestyle factors and metabolic syndrome as well as bioactive compounds in foods for the prevention and treatment of lifestyle-related and non-communicable diseases. She is also involved in delivering nutrition services thru the nutrition  clinic in the University and by serving as technical assistant and resource person to private companies, government and non-government organizations

Abstract:

Introduction: Oxidative stress and inflammation are known to play key roles in both pathogenesis and progression of diabetes mellitus (DM). The study aimed to determine the effects of red raspberries (Rubus ideaus L.) crude extract (RCE) on biomarkers of oxidative stress and inflammation in glucose-induced hyperglycemia in human umbilical vein endothelial cells (HUVECs) that mimicked hyperglycemia in DM. Methodology: The following were determined:1) bioactive compounds in red raspberries including a) dietary fiber; b) antioxidant activity; c) total polyphenol contents; and d)  identity of individual polyphenol, and 2) effects of raspberry crude extracts on biomarkers of oxidative stress and inflammation in glucose-induced hyperglycemic HUVECs in terms of a) ROS scavenging activity; b) lipid peroxidation inhibition activity; and c)  expression of transcription factors nuclear factor (erthyroid derived-2)-like (Nrf2), peroxisome proliferator-activated receptor-gamma (PPAR-γ) and nuclear factor-kB (NF-kB) and cytokine interleukin (IL)-6. Findings: Results revealed that red raspberries contain polyphenols such as quercetin and flavonoids that are known to exert strong antioxidant capacity. Results of in vitro study showed that RCE attenuate oxidative stress by significantly inhibiting reactive oxygen species (ROS) (p<0.05) and lipid peroxidation (P<0.05). However, at higher concentration (20ug/mL), RCE behaved as pro-oxidant and significantly decreased cell proliferation (p<0.05). Also, RCE was observed to interact with molecular signaling pathways that are involved in oxidative stress and inflammation. It significantly up-regulated the expression of transcription factors Nrf2 and PPAR-γ in non-dose dependent manner. Also, RCE significantly down-regulated the expression of pro-inflammatory transcription factor NF-kB and inflammatory IL-6 in dose-dependent manner. Conclusion and Recommendation: Given these findings, in vitro and in vivo studies focused on signaling pathways and molecular targets using different varieties of raspberries merit further investigations

Speaker
Biography:

Dina Keumala Sari has her expertise in vitamin D and polymorphism vitamin D receptor gene. Her interest in vitamin D creates a theory about microevolution in North Sumatera women in Indonesia

Abstract:

Background: A large proportion of women living in tropical regions experience Vitamin D deficiency especially in North Sumatera, Indonesia, and lifestyle being one of the predisposing factors. Other causes may be attributed to the presence of polymorphism in the Vitamin D receptor gene TaqI and BsmI.

Objective: To assess the effect of 1000 IU vitamin D supplementation on 25(OH)D serum and calcium levels in North Sumatera women with vitamin D re ceptor gene polymorphism (TaqI and BsmI).

Design: The study was a parallel clinical trial, thirtysix subjects with vitamin D receptor gene polymorphism (TaqI and BsmI) were selected using certain criterias. The subjects were devided into two groups using block randomization. Nineteen subjects in vitamin D group received 1000 IU vitamin D supplementation per day and dietary counseling (D), while seventeen subjects in counseling group received placebo and dietary counseling (C). Serum 25-hydroxyvitamin D and calcium levels were assessed on day 0 and 28. Before and after treatment, dietary intake were assessed with 2x24 hour recal methods.

Results: Nineteen subjects in D group and seventeen subjects in C group completed the study. There was no significant differences in baseline data both groups. After 28 days treatment, there was no significant differences in nutrients intake in two groups except vitamin D intake in D group. There was significant increase in 25(OH)D serum level in D group while no change was observed in the C group (p=0.04). Serum calcium concentration showed no significant change in both groups. There was significant differences between both group but all subjects did not reach normal value in 25(OH)D serum level (normal value >30 ng/mL).

Conclusions: The result shows that vitamin D deficiency can occur in women with Vitamin D receptor gene polymorphism even already supplemented with 1000 IU vitamin D.

Speaker
Biography:

Cherry Ann Garcia is a Master in Science in Public Health Major in Nutrition. She worked as a Nursing Review Lecturer for Medical-Surgical Nursing and Maternal and Child Nursing. 

Abstract:

In the Philippines, 2-3 out of 10 children suffer from atopic dermatitis (AD) or eczema. The most common treatment of atopic dermatitis is the application of topical corticosteroids especially for acute exacerbation. Antihistamine is also given to reduce the pruritus. However, despite the presence of pharmacological management, prevention of attacks by identifying and avoiding triggering factors is the focus of health teaching for parents of children with AD. Food allergy is found to co-exist in approximately 40% of children with moderate to severe AD. Nonetheless, potential food allergens can be difficult to avoid because most common food triggers are egg, milk, wheat, peanut, and soy, which are used in many food preparations. For this reason, the research community is investigating possible innovative prevention and treatment strategies for atopic dermatitis. One of these strategies is the manipulation of the intestinal flora through probiotics. Probiotics are defined by the FAO/WHO as “live microorganisms which when administered in adequate amounts, confer a health benefit on the host.” One of the biggest companies that supplies probiotic drink in the country released their annual report in 2014, and about 15% of the total population avails of the probiotic drink with average sales of 15.9 million bottles in one day. Despite of this data, there is very little research on the specific reasons why consumers avail of probiotics. Furthermore, the perspective of mothers on probiotics and its use is an area of research that is not yet explored, evidenced by the lack of published works in local peer-reviewed journals. Through a face-to-face interview using a guide questionnaire, this cross-sectional study gathers information on maternal perspectives on probiotics. A food frequency questionnaire is used to determine the frequency and amount of probiotic food consumption by the child. Using the diagnostic criteria in the ISAAC (International Study of Asthma and Allergies in Childhood) questionnaire, a medical doctor collects data on eczema. The results will hopefully reveal the effect of probiotic consumption to the occurrence of atopic dermatitis in children age four to five.

Speaker
Biography:

Nindy Sabrina is a second year master student in the School of Nutrition and Health Sciences, Taipei Medical University, Taiwan. Nindy originally from Malang, Indonesia. She received her undergraduate education at Brawijaya University, Indonesia, with a degree in Nutrition and Health. Currently, she is actively involved in Indonesia Student Association in Taiwan. Nindy is broadly interested in human nutrition research particularly obesity, iron, and advanced glycation end products. In her master research, she is seeking to understand the relationships between dietary intake, serum iron biomarkers and advanced glycation end products in Non-Alcoholic Fatty Liver Disease (NAFLD) patient in Taipei

Abstract:

Background: Recent evidence suggests that calorie restriction (CR) is more effective for obesity treatment that dietary fat restriction. Meal replacement (MR) also have shown as an effective strategy for the long term maintenance of weight loss compared with a traditional reduced calorie diet regimen. However, it is not clear whether combination between calorie restriction with meal replacement diet (CRMR) would promote favorable change in body weight and body composition. The aim of this study was to compare the effects of dietary intervention focusing on either calorie restriction alone or calorie restriction plus meal replacement on weight loss and body composition.

Subjects/Methods: The 99 eligible subjects were randomized to either 1500-kcal CR and CRMR groups for 12 weeks. The CRMR group was supplied with low-calorie nutrition drink mix powder. The changes in anthropometry and body composition were evaluated. Body composition was measured with dual energy x-ray absorptiometry (DEXA).

Results: Seventy subjects have completed the study. During weight loss period, body weight and body mass index (BMI) decreased similarly (P<0.001) in the CRMR group (-7.39 ± 5.09 %;-7.40 ± 5.10 %, respectively) and CR group (-2.31 ± 2.07 %; -2.31 ± 2.09 %, respectively). Waist circumference decreased similarly (P<0.001) in the CRMR and CR groups (-6.80 ± 7.59 % and -5.96 ± 4.80 % respectively). Fat mass decreased more (P<0.001) in the CRMR group (-15.99 ± 9.37 %) versus the CR group (-4.20 ± 6.82). Android fat and gynoid fat decreased similarly (P<0.001) in the CRMR group by -7.44 ± 7.82 % and -3.26 ± 7.16 %, respectively.

Conclusion: These findings suggest that CRMR is effective to body weight and body composition of obese in comparison with CR alone