Liezl M. Atienza
University of the Philippines Los Banos, Philippines
Title: Red Raspberry Crude Extracts (Rce) Reduced Oxidative Stress And Inflammation In Human Umbilical Vein Endothelial Cell (Huvec) Induced By Glucose
Biography
Biography: Liezl M. Atienza
Abstract
Introduction: Oxidative stress and inflammation are known to play key roles in both pathogenesis and progression of diabetes mellitus (DM). The study aimed to determine the effects of red raspberries (Rubus ideaus L.) crude extract (RCE) on biomarkers of oxidative stress and inflammation in glucose-induced hyperglycemia in human umbilical vein endothelial cells (HUVECs) that mimicked hyperglycemia in DM. Methodology: The following were determined:1) bioactive compounds in red raspberries including a) dietary fiber; b) antioxidant activity; c) total polyphenol contents; and d) identity of individual polyphenol, and 2) effects of raspberry crude extracts on biomarkers of oxidative stress and inflammation in glucose-induced hyperglycemic HUVECs in terms of a) ROS scavenging activity; b) lipid peroxidation inhibition activity; and c) expression of transcription factors nuclear factor (erthyroid derived-2)-like (Nrf2), peroxisome proliferator-activated receptor-gamma (PPAR-γ) and nuclear factor-kB (NF-kB) and cytokine interleukin (IL)-6. Findings: Results revealed that red raspberries contain polyphenols such as quercetin and flavonoids that are known to exert strong antioxidant capacity. Results of in vitro study showed that RCE attenuate oxidative stress by significantly inhibiting reactive oxygen species (ROS) (p<0.05) and lipid peroxidation (P<0.05). However, at higher concentration (20ug/mL), RCE behaved as pro-oxidant and significantly decreased cell proliferation (p<0.05). Also, RCE was observed to interact with molecular signaling pathways that are involved in oxidative stress and inflammation. It significantly up-regulated the expression of transcription factors Nrf2 and PPAR-γ in non-dose dependent manner. Also, RCE significantly down-regulated the expression of pro-inflammatory transcription factor NF-kB and inflammatory IL-6 in dose-dependent manner. Conclusion and Recommendation: Given these findings, in vitro and in vivo studies focused on signaling pathways and molecular targets using different varieties of raspberries merit further investigations