Scientific Program

Conference Series LLC Ltd invites all the participants across the globe to attend 20th World Congress on Nutrition & Food Sciences Tokyo, Japan.

Day 1 :

Keynote Forum

Almagul Kushugulova

Nazarbayev University, Astana, Kazakhstan

Keynote: Clinical efficiency of mare's milk in children

Time : 09:30-10:10

Nutrition 2018 International Conference Keynote Speaker Almagul Kushugulova  photo
Biography:

Almagul Kushugulova has completed her PhD at the age of 25 years from Astana Medical State University and taken Degree of D.M.Sc at National Biotechnological Centre. She is a head of Human Microbiome Lab of National laboratory Astana Nazarbayev University. She has published more than 100 papers in Kazakhstan journals, 15 in reputed Journals and has been serving as an editorial board member of Journal of Clinical Medicine of Kazakhstan. 

Abstract:

Background: The main task of joint health care is the prevention of diseases. Functional products play a significant role in preventive medicine. One such promising nutraingredient is the freeze-dried mare's milk. Due to its physiological, balanced nutrient composition, easy digestibility, the mare's milk can be used with great benefit in dietary regimens when a diet is required, which as a result helps to cleanse the body, normalize metabolism, improve blood circulation and, to some extent, slow the aging process.

Objective: The purpose of the work is to study the clinical effectiveness of freeze-dried mare's milk in inflammatory bowel diseases and chronic constipation because of the dolechosigma.

Methods: The company-producer was granted freeze-dried mare milk "Saumal". A pilot study was conducted on a two groups of children 9-12 years old with inflammatory bowel diseases and chronic constipation because of the dolechosigma.

Results: In the first group, the product as an additional food was given to children aged 9-12 years with ulcerative colitis; children had a body mass deficit of more than 10%. Patients with this disease are contraindicated in the use of cow's milk, while at the same time requires a high-calorie diet with high protein content. As a result, after 90 days of application of the product, were observed the weight stabilization with a moderate increase of 500 g.

In the second group, the product was given to children aged 9-12 years, with constipation, with diagnosis of dolichosigma, with unsiignificant effect from standard therapy. Normalization of stool frequency and consistency during the few weeks from the beginning of mare's milk was noted, no side effects were observed.

Conclusion: Thus, the testing of freeze-dried mare's milk "Saumal" showed good clinical efficacy in children with inflammatory bowel diseases and chronic constipation. Further in-depth studies of the effectiveness of the therapeutic use of mare's milk in children are needed

Nutrition 2018 International Conference Keynote Speaker Claude Billeaud photo
Biography:

Claude Billeaud received his MD degree from the Medical University of Bordeaux ( France) in 1979 after a graduation in human cytogenetics (1976). He then studied pediatrics and has been the Clinical Assistant Director of Bordeaux University in the departments of Pediatrics, Neonatology and Intensive Care since 1983. He currently serves as a pediatrician in the neonatal unit at the Children’s Hospital of Bordeaux, as a scientific manager of Bordeaux-Marmande human milk bank, as a lecturer and head of research (HDR : Habilitation to direct research) in neonatal nutrition at the Medical University of Bordeaux. His particular interest in research led him to graduate in Biology and Health (1988, Bordeaux), be awarded a master in statistics applied to clinical research ( 1991, Montreal) and complete a PhD in nutrition and food science (2000, Bordeaux). Along his career he has often been invited as a guest professor specialised in nutrition and neonatology in various universities abroad ( Montreal, Corrientes in Argentina). Over the last 35 years, he has been an active member of different scientific organisations, either French, European or American, specialised in perinatal medicine (neonatology, pediatrics and nutrition). In this instance, he has served as the President of the Association for Pediatric Education in Europe (A.P.E.E) since 2008 and behalf APEE he is Member of European Academy of Paediatrics (EAP). He has also been very involved in the French human milk banking association (ADLF) for more than 10 years, sharing his academic knowledge focused in nutrition and his long clinical experience in neonatology. He is currently carrying out several researches on the composition of human milk. As an expert in nutrition and perinatal medicine, he is also the author and co-author of numerous scientific publications.      

Abstract:

Introduction / Objectives :

Polyunsaturatedfattyacids (PUFA) long chain, especially n-6 arachidonicacid (ARA) and the docosahexaenoicacid n-3 (DHA), are important in the development and maturation of the newbornbrainsystem .Their content in human milk (HM) varies with the mother's diet. Supplementationwith n-3 PUFA (α-linolenicacid, ALA) could increase the concentration of DHA in milk. The objective of the study was to assess the composition of breast milk after 15 days supplementation of n-3 PUFAs.

Material and Methods:

Multicentric randomized trial (human milk banks : Bordeaux, Lyon, Paris, Montpellier), according to a factorial design 4 groups of 20 women each. From D0 to D15, same diet (olive oil), and from D15 to D30 : diets were 1) Olive oil (O) 2) margarine rich in n-3 PUFA (M), 3) rapeseed oil (C) and 4) M + C (MC). Diets 1-4 provided an increasing ALA intake. In the 4 groups, there was constant supply of DHA (500mg / d, 170 g Mackerel 2 times / week). The FA composition of milk (% of total FA) was determined by direct transesterification and analyzed by GC-FID, blinded group. Nutritional surveys were analyzed by Bilnut software. FA concentrations at day 30 were compared between groups by linear regression, with a test interaction between M and C.

Results:

80 mothers of term infants were  included: age 31.5 ± 4.2, 66.1 ± 9.8 kg (mean ± SD). ALA was higher in MC (2.2%) C (1.3%) and M (1.1%) groups (p <0.003), vs. group O (0.8%). There was a tendency for the DHA to be more higher in the MC group (0.54%) vs O group (0.39%) (p = 0.11). The ratio LA / ALA was the lowest = 5.5 (p <0.001) in the group MC and bonded to an ALA rate to 2.1%. ARA is the highest in group C (0.37% vs. 0.33% MC, M and O 0.32 0.34%) (p = 0.02).The dietary survey showed a slightly high fat diet compared to RDA.

Conclusion:

We recommend for lactating women, a balanced varied diet consisting of 170g mackere l2 times / week or equivalent., which covers the needs for DHA and ARA. Margarine consumption Omega 3 and rapeseed oil improves the ratio LA / ALA (5.5) the most favorable ratio to increase the synthesis of DHA from ALA.

  • Food Science & Chemistry | Public Health Research | Dietary Supplements & Functional Foods | Probiotics & Prebiotics | Human Nutrition & Dietetics | Clinical Nutrition | Nutraceuticals & Medicinal Foods | Nutrition & Metabolism | Nutritional Epidemiology & Malnutrition

Session Introduction

Ayaulym Nurgozhina

Junior researcher at National Laboratory Astana

Title: Food Frequency Questionnaire and FETA software to assess the dietary intake among Patients with Metabolic Syndrome

Time : .

Speaker
Biography:

Ayaulym Nurgozhina has completed Master of Science in Biomedical and Molecular Sciences Research at King’s College London and Bachelor of Science in Biological Sciences at Nazarbayev University. She is the junior researcher at National Laboratory Astana, established under the aegis of Nazarbayev University and Kazakstani government.
 

 

Abstract:

Patients from 25 to 75 years old with and without metabolic syndrome answered food frequency questionnaire. Total number of participants was 84. Questions covered all food preferences, daily intake number of fruits, beverages, milk products, etc. More than 120 food products are listed in the survey. The aim of the study was to identify intakes of different Vitamins in two groups by age. After questionnaire FETA tool was used to analyze all answers (http://www.srl.cam.ac.uk/epic/epicffq/). It is a tool to calculate nutrient and food group data from food frequency questionnaires. This research offered to see differences in more than 20 Vitamins in all age groups and patients with metabolic syndrome and healthy ones. The results showed differences in Vitamins by groups and gender. Patients with metabolic syndrome consume food almost twice more than healthy patients. Therefore, indicators of vitamins were much higher than normal index. For example, Iron’s Recommended Dietary Allowance is 8 mg however male patients with metabolic syndrome aged 60-75 have 23 mg per day as an average value. Recommended Dietary Allowance of each vitamin and mineral was compared with the values from the food frequency questionnaire. It was very important to find which product is responsible for the appearance of the vitamin index in the FETA software results. Moreover, it was suggested to add some more products which are common for Central Asian population such as horse meat, mare’s milk, etc. The results allowed concluding that a high accuracy of the FFQ FETA was achieved. This tool for converting food frequency questionnaire data into nutrient and food group values can be used widely for different study populations. 

 

Kozhakhmetov Samat

Nazarbayev University, Kazakhstan

Title: Metabiotic based on Saumal (mare's milk)

Time : .

Speaker
Biography:

Samat Kozhakhmetov has completed her PhD at the age of 25 years from S.Seifullin Kazakh Agro Technical University and taken Degree of C.M.Sc at National Biotechnological Centre. He is a senior researcher of Human Microbiome Lab of National Laboratory Astana Nazarbayev University. She has published more than 80 papers in Kazakhstan journals, 10 in reputed Journals.

 

Abstract:

During performance of scientific project on order for government account metabolic compound pill having beside useful properties proven in befor clinical tests, was developed. During additional researches pill formula ont the base of natural mare's milk saumal was advanced. Offered product possesses expressed DNA by protective activities, possesses ability to increase adoptational properties of the organism, eliminates infringements of intestinal microbic bias, improves digestion, vegetable fibres and prebiotic improve motor functions of the intestines.

Results showed that probiotic strain Lactobacillus rhamnosus with DNA – protective action, at multiple application, does not cause infringements a functional condition of the main units and the systems of the organism, nor possesses toxic action.

Conducted before clinical researches of first-aid efficiency of strain Lactobacillus rhamnosus on the model of experimental toxic poisoning tetrachlormethan showed that probitoc Lactobacillus rhamnosus at reception in sufficient concentration is capable to lower disturbing influence of toxic гепатотропных substances to intestines microflora.

Studying of DNA-protective activity of the substance on the basis of living probiotical cells Lactobacillus rhamnosus in vivo, testified that daily reception of the substance “Probiotic withDNA-protective activity” during 7 days after creation of the model of sharp toxic poisoning tetrachlormethan, causes a first-aid efficiency.

New product produced from vegetable, animal and mineral raw materials of natural origin having pharmacological activity. It takes place between medical products and foodstuffs, and is ideally combined with rational therapy.

 

Speaker
Biography:

Almagul Kushugulova has completed her PhD at the age of 25 years from Astana Medical State University and taken Degree of D.M.Sc at National Biotechnological Centre. She is a head of Human Microbiome Lab of National laboratory Astana Nazarbayev University. She has published more than 100 papers in Kazakhstan journals, 15 in reputed Journals and has been serving as an editorial board member of Journal of Clinical Medicine of Kazakhstan. 

Abstract:

Recent studies have shown that probiotic yoghurt consumption may have a positive role in the regulation of body weight as well as act to prevent cardiovascular diseases. Dairy products are a source of high quality protein, and due to the functional properties of live bacteria, yoghurt have unique properties. Objective is to test the efficacy of new a synbiotic formulation in adults with metabolic syndrome.

This randomized double-blind placebo controlled trial was conducted among 180 participants aged 25 to 75 years. Subjects with and without metabolic syndrome were randomly assigned to receive synbiotic or placebo daily for three months.

134 of 180 participants completed the study. Compared with the placebo group, the synbiotic group had significantly decreased total cholesterol.

Our study is the first large clinical trial of probiotics in Kazakhstan and the first study on the new synbiotic yoghurt NAR. The uniqueness of this product lies in the fact that it consists of probiotic component strains isolated from a traditional Kazakh koumiss product. Primarily, the product is aimed at people with digestive problems. Therefore, it is rich in easily digestible trace elements and amino acids, essential for the gut microbiota to flourish. We adhered to best practices for clinical trials to ensure that both investigators and patients were blinded to the study and that the data analysis was carried out by independent statisticians.

The present findings suggest positive influence of synbiotic on controlling excess weight and treating some metabolic disorders in adults.

 

Nilva Kazue Sakomura

Universidade Estadual Paulista “Júlio de Mesquita Filho”, Brazil

Title: Optimal tryptophan intake for laying hens

Time : .

Speaker
Biography:

Nilva Kazue Sakomura has completed her PhD at Universidade Federal de Viçosa and postdoctoral studies at University of Arkansas (USA) and University of KwaZulu-Natal (RSA). She acts as professor at the Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCAV/UNESP and coordinates the Laboratory of Avian Sciences at same university. She participated in the elaboration of the last edition of Brazilian Tables for Poultry and Swine. Her research area is mainly foccused on modeling amino acid requirements of broilers, laying hens and broiler breeders.           

Abstract:

Modern poultry industry has been gradually decreasing crude protein content of poultry diets and meeting poultry amino acid requirement more precisely, in order to save production costs and reduce the negative impact of livestock on environment. In this context, determining tryptophan (Trp) requirements of laying hens could allow further reductions on dietary CP supply. A total of sixty 29-week-old Hysex White laying hens were individually allocated into 6 dietary treatments with 10 replicates. The 6 Trp levels studied in this trial (0.81; 1.06; 1.50; 1.86; 2.05 and 2.30 g/kg) were obtained using the dilution technique. The trial lasted 10 weeks, but only those from the final 4 weeks were used in subsequent analysis. Optimal Trp intake was estimated using polynomial, broken-line regression model and the association between both models. Graded Trp intake elicited a quadratic response on egg production (EP) and egg output (EO), which were maximized at 196 and 208 mg Trp/bird/day, respectively, whereas feed conversion ratio per egg output (FCR) were optimized at 185 mg Trp/bird/day. According to broken-line regression model, the breakpoint estimated for maximum EP and EO; and optimal FCR occurred with a daily Trp intake of 117, 134 and 50 mg/bird. The association between polynomial and broken-line regression models produced the optimal Trp intake of 161, 173 and 66 mg/bird/day for EP, EO and FCR. Based on EO responses estimated by associating both regression models, the optimal Trp intake for laying hens is 173 mg/bird/day.

 

Speaker
Biography:

Khairul Bariah is working at Malaysian Cocoa Board for almost 15 years. Currently she is pursuing PhD in food processing at Universiti Sains Malaysia. 

Abstract:

Fermentation is an important process in cocoa that enable the formation of flavor precursor. The process is influenced by various factors, in which will eventually determine the final flavor quality of dried cocoa beans. Therefore, this study was to evaluate the effect of different fermentation duration using a shallow box on the volatile compounds of the dried cocoa beans. The fermentation was conducted at the Cocoa Research and Development Center, Bagan Datuk using 150 kg of fresh cocoa seeds. During fermentation, 15 kg of wet beans was randomly taken out at 0, 24, 48, 72, 96 and 120 hours of duration and subsequently sun-dried until the moisture content reduced to 7.5%. The volatile compounds of cocoa powder prepared from samples were extracted by Solid Phase Micro Extraction using 65μm Polydimethylsiloxane-divinylbenzene coating fiber and analyzed in Gas Chromatography system equipped with Mass Spectrometer Detector. The study identified a total of 126 compounds in the 6 samples of dried cocoa beans which classified into 20 groups. Esters, acids, hydrocarbons, ketones and pyrazines were presented as the main volatiles with more than 10 compounds in each groups. Cocoa beans which fermented for 48 hours using shallow box exhibited highest number of volatiles which associated with cocoa specific aroma such as ethyl laurate, phenethyl acetate, ethyl cinnamate, methyl cinnamate,isoamyl acetate, octanoic acid, a-ethylidene benzeneacetaldehyde, benzeneacetaldehyde and phenylethyl alcohol.

 

Speaker
Biography:

Thilini Keerthirathne is currently a PhD student in the College of Science and Engineering, Flinders University, South Australia.  She has obtained her B.Sc. in Genetics from the University of Bangalore, India in 2010 and completed her postgraduate studies in Biochemistry and Molecular Biology at the University of Colombo, Sri Lanka. She was working as a research assistant at the National Institute of Fundamental studies, Kandy, Sri Lanka before coming to Australia. She has published four peer-reviewed journal articles, one extended abstract, 4 conference abstracts and one conference poster in national and international conferences.

 

Abstract:

In Australia the incidence of salmonellosis is increasing with Salmonella Typhimurium linked to eggs and raw egg products identified as the main cause. Australian guidelines recommend raw egg mayonnaise is prepared and stored under 50C and adjusted to a pH of 4.6. This study investigated the combined effect of pH and temperature on the survival of S. Typhimurium. Nutrient broths with a pH of either 4.2, 4.4, 4.6, or 7.0 (adjusted with acetic acid) were inoculated with 1 mL log phase S. Typhimurium (105 CFU/ mL) and were incubated at either 370C, 230C or 40C. All experiments were conducted in triplicate. The survival of Salmonella over time was determined using the drop plate method on X.L.D. agar, also conducted in triplicate. Survival was significantly (P<0.05) improved when cultures were incubated at 40C compared to 230C and 370C for all pH conditions tested. After 24 hours of incubation at 370C, there were no viable Salmonella at pH 4.2 and 4.4 but there was survival at 230C and 40C for the same pH conditions. The maximum survival was at 4 0C. In conclusion, lower temperatures protected S. Typhimurium from the bactericidal effect of low pH.

 

Speaker
Biography:

Wan Aidah Wan Ibrahim has a degree in Bachelor of Science (Hons) in Food Science from University of Leeds, U.K and a Master of Science in Food Technology from University Kebangsaan Malaysia. She is currently working as a Research Officer with Malaysian Cocoa Board in Cocoa Downstream Technology division.

 

Abstract:

Polyols are commonly used to replace sucrose in the production of chocolate that is low in calorie, tooth friendly and suitable for diabetics. Three types of polyols; isomalt, maltitol and erythritol, as well as sucrose were used in pilot plant scale production of milk chocolate. Sensory evaluation was carried out using quantitative descriptive analysis by 10 trained panelists. Result showed that replacing sucrose with different types of polyols did not significantly (p≥0.05) affects the cocoa taste, sweetness, milky taste, smoothness and melting behavior of the chocolates in the mouth. Consumer test from 100 respondents indicated that chocolate with isomalt and maltitol were equally preferred while chocolate with erythritol was less preferred mainly due to the cooling sensation detected in this sample. The processing parameters used in this study were able to produce sucrose free chocolates with comparable particle size distribution to chocolate with sucrose (measured by Malvern particle size analyser, UK). Hardness analyses measured at 24°C using an SMS Texture Analyzer  (TA.XT plus, UK) showed that freshly made milk chocolate with maltitol and isomalt were percieved to be significantly (p≤0.05) harder to break compared to chocolate with erythritol and sucrose. Chocolate containing isomalt and maltitol displayed a quite similar rheological behavior (by HADV 11+ Brookfield viscometer, UK) and chocolate with erythritol showed a closer rheological property to that of sucrose. Color (by Hunterlab MiniScan XE Plus, USA) and surface gloss (by Sheen Tri-microgloss, UK) analyses showed that the physical appearance of chocolates with polyols were fairly stable when stored at chill temperature (16±1°C).

 

Speaker
Biography:

Wiphada Mitbumrung graduated bachelor’s degree from Department of Biotechnology, Faculty of Science, Mahidol University. At present, she is a master’s degree student in food science for nutrition program at Institute of Nutrition, Mahidol University. Research interest is an application of natural hydrocolloid and Application of emulsion for nutrient and non-nutrient delivery system. 

 

Abstract:

Microfibrillated cellulose (MFC) was an expanded cellulose prepared by hot alkali pretreatment and mechanical force. Normally, MFC was extracted from agricultural sources so, this study used mangosteen rind for MFC production because it was a by-product from the food industry.MFC could be used as a natural emulsifier for encapsulation of fat-soluble vitamin in emulsion system. The aim of this study was to investigate the effect of MFC concentration on emulsion properties and stability. Oil-in-water (O/W)emulsion; oil phase (0.01% vitamin D3, 9.99% soybean oil), aqueous phase (MFC, 10mM phosphate buffer pH7) was performed by using different MFC concentration (0.3, 0.5, 0.7% w/w) using a 2-stage high pressure homogenizer. Encapsulation efficiency, oil droplet size, color, ζ-potential, microstructure, and creaming stability of the emulsion samples were measured. The result found that MFC concentration affected to emulsion properties and stability. The emulsion containing 0.3, 0.5 and 0.7% MFC expressed encapsulation efficiency 87, 93 and 96%, respectively. All emulsions exhibited mean droplet diameter in range 10 to 100 µm. The increasing of MFC concentration exhibited larger oil droplet, provided darker color and increased magnitude of ζ-potential. SEM micrograph showed MFC formed physical barrier around oil droplet which provided stability of emulsion droplets against coalescence. However, it was found phase separation at concentration 0.3%MFC but did not cream at concentration 0.5 and 0.7%MFC throughout 90 days.

 

Nilva Kazue Sakomura

Universidade Estadual Paulista “Júlio de Mesquita Filho”, Brazil

Title: Ideal in-feed amino acid ratio for laying hens

Time : .

Speaker
Biography:

Nilva Kazue Sakomura has completed her PhD at Universidade Federal de Viçosa and postdoctoral studies at University of Arkansas (USA) and University of KwaZulu-Natal (RSA). She acts as professor at the Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCAV/UNESP and coordinates the Laboratory of Avian Sciences at same university. She participated in the elaboration of the last edition of Brazilian Tables for Poultry and Swine. Her research area is mainly foccused on modeling amino acid requirements of broilers, laying hens and broiler breeders.           

Abstract:

The research was conducted to re-evaluate the assumptions of ideal ratios (IAAR) of the essential amino acids for Hy-line W-36 laying hens, using the amino acid deletion and nitrogen balance methods. The nitrogen balance trial was performed from 28 to 32 weeks of age, 12 treatments with 8 replicates and 1 bird per cage were used. A balanced diet (BD) was formulated to meet the IAAR and the requirement of other nutrients. The limiting diets were formulated diluting BD with corn starch and refilled with synthetic amino acids (AA) and other feed ingredients, except for the AA studied. The trial lasted 25 d, being the first 5 d for adaptation and the other 20 d for total collection of excreta and eggs. Diets, excreta and eggs samples were analyzed for nitrogen content to quantify nitrogen intake and excretion. From these data, nitrogen retention (NR) was calculated. The estimated requirement of each AA (AAR) was calculated as AAR= 100 x (1-NRi/NRcontrol) where NRi is the NR associated with each limiting diet calculated and NRcontrol is the NR associated with the balanced control diet. The estimated optimum ratio between each AA and Lys [(AA/Lys) * 100] and the optimum level of each AA (values in parentheses) were: Lys = 100 (0.673), Met+Cys = 82 (0.552), Thr = 69 (0.464), Trp = 21 (0.141), Arg = 104 (0.700), Val = 91 (0.612), Ile = 78 (0.525), Leu = 121 (0.814), Phe+Tyr = 119 (0.801), Gly+Ser = 77 (0.518) and His = 29 (0.195).