Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 20th World Congress on Nutrition & Food Sciences Tokyo, Japan.

Day 1 :

Keynote Forum

Claude Billeaud

Ex-President, Association for Pediatric Education in Europe

Keynote: Randomized Trial of Nutritional Intervention Evaluating the Effect of Rapeseedoil, Margarine Enriched

Time : 09:00-09:40

Nutrition 2018 International Conference Keynote Speaker Claude Billeaud photo
Biography:

Claude Billeaud received his MD degree from the Medical University of Bordeaux ( France) in 1979 after a graduation in human cytogenetics (1976). He then studied pediatrics and has been the Clinical Assistant Director of Bordeaux University in the departments of Pediatrics, Neonatology and Intensive Care since 1983. He currently serves as a pediatrician in the neonatal unit at the Children’s Hospital of Bordeaux, as a scientific manager of Bordeaux-Marmande human milk bank, as a lecturer and head of research (HDR : Habilitation to direct research) in neonatal nutrition at the Medical University of Bordeaux. His particular interest in research led him to graduate in Biology and Health (1988, Bordeaux), be awarded a master in statistics applied to clinical research ( 1991, Montreal) and complete a PhD in nutrition and food science (2000, Bordeaux). Along his career he has often been invited as a guest professor specialised in nutrition and neonatology in various universities abroad ( Montreal, Corrientes in Argentina). Over the last 35 years, he has been an active member of different scientific organisations, either French, European or American, specialised in perinatal medicine (neonatology, pediatrics and nutrition). In this instance, he has served as the President of the Association for Pediatric Education in Europe (A.P.E.E) since 2008 and behalf APEE he is Member of European Academy of Paediatrics (EAP). He has also been very involved in the French human milk banking association (ADLF) for more than 10 years, sharing his academic knowledge focused in nutrition and his long clinical experience in neonatology. He is currently carrying out several researches on the composition of human milk. As an expert in nutrition and perinatal medicine, he is also the author and co-author of numerous scientific publications.      

Abstract:

Introduction / Objectives :

Polyunsaturatedfattyacids (PUFA) long chain, especially n-6 arachidonicacid (ARA) and the docosahexaenoicacid n-3 (DHA), are important in the development and maturation of the newbornbrainsystem .Their content in human milk (HM) varies with the mother's diet. Supplementationwith n-3 PUFA (α-linolenicacid, ALA) could increase the concentration of DHA in milk. The objective of the study was to assess the composition of breast milk after 15 days supplementation of n-3 PUFAs.

Material and Methods:

Multicentric randomized trial (human milk banks : Bordeaux, Lyon, Paris, Montpellier), according to a factorial design 4 groups of 20 women each. From D0 to D15, same diet (olive oil), and from D15 to D30 : diets were 1) Olive oil (O) 2) margarine rich in n-3 PUFA (M), 3) rapeseed oil (C) and 4) M + C (MC). Diets 1-4 provided an increasing ALA intake. In the 4 groups, there was constant supply of DHA (500mg / d, 170 g Mackerel 2 times / week). The FA composition of milk (% of total FA) was determined by direct transesterification and analyzed by GC-FID, blinded group. Nutritional surveys were analyzed by Bilnut software. FA concentrations at day 30 were compared between groups by linear regression, with a test interaction between M and C.

Results:

80 mothers of term infants were  included: age 31.5 ± 4.2, 66.1 ± 9.8 kg (mean ± SD). ALA was higher in MC (2.2%) C (1.3%) and M (1.1%) groups (p <0.003), vs. group O (0.8%). There was a tendency for the DHA to be more higher in the MC group (0.54%) vs O group (0.39%) (p = 0.11). The ratio LA / ALA was the lowest = 5.5 (p <0.001) in the group MC and bonded to an ALA rate to 2.1%. ARA is the highest in group C (0.37% vs. 0.33% MC, M and O 0.32 0.34%) (p = 0.02).The dietary survey showed a slightly high fat diet compared to RDA.

Conclusion:

We recommend for lactating women, a balanced varied diet consisting of 170g mackere l2 times / week or equivalent., which covers the needs for DHA and ARA. Margarine consumption Omega 3 and rapeseed oil improves the ratio LA / ALA (5.5) the most favorable ratio to increase the synthesis of DHA from ALA.

Keynote Forum

Osama O Ibrahim

BioInnovation LLC, United States

Keynote: Oligosaccharides: Chemicals Structure, Manufacturing Process, Regulations, and Applications

Time : 09:40-10:20

Nutrition 2018 International Conference Keynote Speaker Osama O Ibrahim photo
Biography:

Osama O Ibrahim is a highly-experienced Principal Research Scientist with particular expertise in the field of microbiology, molecular biology, food safety and bioprocessing for both pharmaceutical and food ingredients. He is knowledgeable in microbial screening, culture improvement; molecular biology and fermentation research for antibiotics, enzymes, therapeutic proteins, organic acids and food flavors; biochemistry for metabolic pathways and enzymes kinetics, enzymes immobilization, bioconversion and analytical biochemistry. He was an External Research Liaison for Kraft Foods with Universities for research projects related to molecular biology and microbial screening and holds three bioprocessing patents and multiple publications. Upon his retirement from Kraft Foods he established his own biotechnology company providing technical and marketing consultation for new startup biotechnology and food companies. He has received his BS in Biochemistry with honor and two MS degrees in Microbial Physiology/Fermentation and in Applied Microbiology. He has received his PhD in Basic Medical Science (Microbiology, Immunology and Molecular biology) from New York Medical College. He is a Member of American Chemical Society, American Society of Microbiology and Society of Industrial Microbiology since 1979.

Abstract:

Oligosaccharides are carbohydrates that have three to six units of simple sugars (monosaccharides). They are one of the components of fibers, found in many plants with large amounts include Jerusalem artichokes from which most commercial inulin is extracted. They are also found in onions, garlic, legumes, wheat, asparagus and other plant foods.  Most oligosaccharides have a mildly sweet taste and have certain other characteristics, such as mouth feel they lend to food. This mouth feels characteristic interest food industry to add oligosaccharides in some foods as a partial substitute for fat and sugars and to improve texture.  Because 90 % of oligosaccharides escapes digestion in small intestine  and reach  the clone where it  perform  a different function  as a growth factor  (prebiotics) that enhance the growth of beneficial bacteria (probiotics) in the colon. This recent benefit has increased the global market for oligosaccharides. Because increasing demand of oligosaccharide more and more of the oligosaccharides are synthetically produced as a replacement to plants extraction methods. Properties, benefits, legal status and manufacturing process for oligosaccharides available in the market will be highlighted in this presentation.

  • Food Science & Chemistry | Public Health Research | Dietary Supplements & Functional Foods | Probiotics & Prebiotics | Human Nutrition & Dietetics | Clinical Nutrition | Nutraceuticals & Medicinal Foods | Nutrition & Metabolism | Nutritional Epidemiology & Malnutrition

Session Introduction

Dean A. Sewell

Heriot-Watt University, School of EGIS, Edinburgh, U.K.

Title: Towards reducing allergy prevalence from an early age
Speaker
Biography:

Dean Sewell is an experienced researcher and teacher with experience in both academia and industry. He is a Registered Nutritionist, and a former Honorary External Affairs Officer of The Nutrition Society (UK). Collaboration has resulted in a network of contacts locally, nationally and internationally.

 

Abstract:

         IgE-mediated allergy such as eczema, food allergy, hayfever and asthma may affect 50% of the paediatric population. Diet may have a role in the primary prevention of allergy as the pre-natal period and the first few months of life are a critical period when there can be modulation of growth and development of the respiratory, immune and gastrointestinal systems.  It has been hypothesised that maternal diet during pregnancy modulates the development of allergy and asthma by influencing foetal airway and/or immune development.  Reducing or delaying allergic disease onset in early life could have a significant public health impact.

Our programme of work to date includes an extensive systematic review and meta-analysis examining the strength of the scientific evidence for associations between dietary intake of foods and nutrients by pregnant women and children and the risk of children developing allergy and asthma.  Results found a potentially protective role for candidates including the Mediterranean diet (MD), and we hypothesise that adherence to a MD might represent an effective primary prevention strategy.  Although observational studies have reported potentially beneficial associations with a MD, it remains to be seen whether an intervention to promote the MD reduces the likelihood of childhood allergy and asthma.  This needs to be investigated through formal experimental studies, as there are currently no randomised controlled trials (RCT’s) testing the hypothesis that enhancing MD adherence in the mother will decrease the risk of allergic disease in children.  We have engaged in a programme of work to design an adequately powered RCT to investigate the potential protective effects of the MD on the risk of developing allergy and asthma. Our pilot RCT investigated maternal recruitment and retention to a MD intervention and we have published the protocol and results, and shortly, a follow-up of the infants born to women who participated in the pilot RCT. Infant follow-up included allergic outcomes at 6 months determined through birth and environment data, and at 12 months of age determined through clinical history, allergen skin prick testing (SPT) and clinical examination.

We will soon begin a well-designed and adequately powered RCT to investigate the potential protective effects of the MD on the risk of developing allergy and asthma, following a framework for developing and evaluating complex interventions.

 

Speaker
Biography:

Thilini Keerthirathne is currently a PhD student in the College of Science and Engineering, Flinders University, South Australia.  She has obtained her B.Sc. in Genetics from the University of Bangalore, India in 2010 and completed her postgraduate studies in Biochemistry and Molecular Biology at the University of Colombo, Sri Lanka. She was working as a research assistant at the National Institute of Fundamental studies, Kandy, Sri Lanka before coming to Australia. She has published four peer-reviewed journal articles, one extended abstract, 4 conference abstracts and one conference poster in national and international conferences.

 

Abstract:

In Australia the incidence of salmonellosis is increasing with Salmonella Typhimurium linked to eggs and raw egg products identified as the main cause. Australian guidelines recommend raw egg mayonnaise is prepared and stored under 50C and adjusted to a pH of 4.6. This study investigated the combined effect of pH and temperature on the survival of S. Typhimurium. Nutrient broths with a pH of either 4.2, 4.4, 4.6, or 7.0 (adjusted with acetic acid) were inoculated with 1 mL log phase S. Typhimurium (105 CFU/ mL) and were incubated at either 370C, 230C or 40C. All experiments were conducted in triplicate. The survival of Salmonella over time was determined using the drop plate method on X.L.D. agar, also conducted in triplicate. Survival was significantly (P<0.05) improved when cultures were incubated at 40C compared to 230C and 370C for all pH conditions tested. After 24 hours of incubation at 370C, there were no viable Salmonella at pH 4.2 and 4.4 but there was survival at 230C and 40C for the same pH conditions. The maximum survival was at 4 0C. In conclusion, lower temperatures protected S. Typhimurium from the bactericidal effect of low pH.

 

Speaker
Biography:

Dr Xiao Su is an Associate Professor in the Victoria University, Australia. She completed her PhD study and postdoctoral training at the University of Tasmania, Hobart, Australia in 1995. She then took an academic position at the Victoria University, Australia and has worked there since 1996. Dr Su’s research focuses on the therapeutic role of natural products in chronic disease including obesity, diabetes and cancer. She has received several national and international research grants, and has published widely in the quality scientific journals. She has been invited to present at the international conference and is a reviewer of several international journals.  

 

Abstract:

Colorectal cancer (CRC) is one of the major causes of cancer death worldwide. The currently available chemotherapy is associated with numerous side-effects. Therefore, the effective nutraceutical agents with low or no side-effects are desirable. Krill oil (KO) is a rich source of long-chain omega-3 polyunsaturated fatty acids, mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Our preliminary data showed that the free fatty acid extract (FFAE) from the KO suppresses cell growth and induces apoptosis of human CRC cells. The aim of this study was to investigate the effect of FFAE from KO and potential mechanism underlying it’s anti-cancer effect on human CRC cells. DLD-1, HT-29 and LIM-2405 cell lines were treated with EPA (50 - 200 µM), DHA (50 - 300 µM) and FFAE of krill oil (0.03 - 0.24 µL/100µL) for 24h and 48h to evaluate their effect on cell proliferation using a water-soluble tetrazolium-1 (WST-1) assay kit. The effect of FFAE from KO (0.12 µL/100µL), EPA (200 µM) and DHA (250 µM) on mitochondrial membrane potential was determined using a JC10 mitochondrial assay kit, and the effect on reactive oxygen species (ROS) was determined using a MitoSOX™ Red M36008 kit. The FFAE of KO (0.06 - 0.24 µL), EPA (200 µM) and DHA (200 - 300 µM) significantly inhibited the cell proliferation in all three CRC cell lines. Moreover, they have reduced the formation of ROS. Treatment with the FFAE of KO also resulted in a significant increase in the mitochondrial membrane potential. However, no such change was observed after EPA and DHA treatments. These results suggest that the anti-proliferative effect of FFAE from KO may be associated with the intrinsic (mitochondrial) pathway although other active components are yet to be determined. The supplementation with krill oil may provide a novel and safe therapy for CRC treatment.  

 

Speaker
Biography:

Fatemeh Mahmoodani is currently a final year PhD student at the School of Chemical Sciences, University of Auckland, New Zealand. Her current research focuses on vitamin D3 degradation in whole milk powder and identification of vitamin D3 isomerization and oxidation products. Her PhD project is collaboration with Fonterra Cooperative Group Ltd. and funded by the Primary Growth Partnership Program of New Zeeland.

Abstract:

In a successful fortification program, the stability of micronutrients added to the food is one of the most important factors. The added vitamin D3 is known to sometimes decline during storage of fortified milk powders, and oxidation through fatty acid lipoxidatoin is suspected as the likely cause. The main objective of this study is to find a method to extract and to identify vitamin D3 oxidation products (VDOPs) in stored whole milk powder. Identification of VDOPs in natural foods is a challenge due to the low amount of their contents. An extraction method using a liquid-liquid extraction (LLE) followed by a solid phase extraction (SPE) was optimized to extract VDOPs. The bottleneck of the VDOPs’ identification could not be overcome without mass spectra prediction tools. The fragmentation trees and MSn spectral trees provided by Mass Frontier software gave reliable methods to identify vitamin D3 unknown compounds such as VDOPs. Methods based on high mass accuracy MS2 and MSn analysis have been developed for the identification of vitamin D3 and its oxidation products. The multi stage mass spectrometry (MSn) spectra can help to propose plausible schemes for unknown compounds and their fragmentations. This study was focused on identifying the fragmentation rules for some VDOPs by incorporating MS data with in silico calculated MS fragmentation pathways. Diels-Alder derivatization was used to enhance the sensitivity and selectivity for mass spectrometry data collection. Finally, the confirmed PTAD derivatised target compounds were separated and analyzed using ESI(+)-UHPLC-MS/MS in multiple reaction monitoring (MRM) mode in model samples.

 

Speaker
Biography:

Xiaofang Jia has completed her PhD in the Graduate School of Medicine, Nagoya University, Japan. Currently, she is an associate professor in National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, and interested in the field of Nutrional Epidemiology. She has published more than 10 papers in reputed journals. 

Abstract:

Vitamin C is essential for human health. However, dietary vitamin C intake in Chinese population have changed with nutritional transition in recent decades and needs to be estimated. Present study used data of “China Nutritional Transition Cohort Study” in 2015 and selected those aged 18-65 years with completed data of socio-demographic characteristics, anthropometry and dietary measurements as subjects. We analyzed daily dietary vitamin C intake and food sources across demographic factors, evaluated the prevalence of vitamin C deficiency using the estimated average requirement cut-off point, and explored underlying influencing factors. Mean and median levels of dietary vitamin C intake in adults was 78.1 and 65.4 mg/day, respectively. Prevalence of vitamin C deficiency in study population and proportion of subjects with lower probability of vitamin C inadequacy were 65.1% and 25.9%, respectively. Both the distribution of vitamin C intake and the prevalence of vitamin C deficiency differed by gender, educational level, residence area, geographic location, smoking, alcohol intake and vegetable consumption frequency. Moreover, gender, educational level, residence area, geographic location and vegetable consumption were found to be independent determinants for vitamin C deficiency. Top four food sources of vitamin C in adults were light vegetables, dark vegetables, fruits and tubers, totally contributing 97.3% to total vitamin C. In conclusion, dietary vitamin C deficiency is severe in Chinese adults. Vitamin C intake on a recommended basis in Chinese population is required, especially women and population living in town and rural regions.

 

LI LI

National Institute for Nutrition and Health, China.

Title: Thiamin, Riboflavin and Niacin intakes in Chinese adults in 15 provinces
Biography:

LI Li had graduated from the London School of Hygiene & Tropical Medicine in 2005 with a master's degree in Medical Parasitology. From 1996 to 2012, She mainly engaged in infectious diseases control, scientific research and teaching in province level. Since 2013, she joined in China CDC, and mainly involved in food safety, foodborne diseases control and nutrition epidemiology in National Institute of Nutrition and Health, China CDC. She has published more than 8 papers in reputed journals.

 

Abstract:

B vitamins have been neglected in China. Most people who lack it belong to subclinical conditions. Well understanding the prevalence of B vitamins will provide guidance for nutritional policies and interventions in China. The data were used from a longitudinal survey in 2015, China health and nutrition survey (CHNS) among 18-64 year old. Dietary data of subjects on three consecutive days were recorded. The EAR cut-point method (EAR cut-point) was used to assess the prevalence of the adequacy intake of three B vitamins, thiamin, riboflavin and niacin by sex, age, areas of residence, south and north parts of China, education, and income. For male, the prevalence of inadequate intake of vitamin B1,B2 and niacin was 77.39%,85.47% and 25.60% respectively. For female, the prevalence of inadequate intake of vitamin B1, B2 and niacin was 75.84%,81.71% and 26.49%, respectively. Vitamin B1 and B2 are seriously deficient in both sex in 18-64 year old adults. Better income or better education levels of both sex have higher intake of vitamin B2 and niacin than those with lower incomes or education. The intake of vitamin B1, B2 and niacin is inadequate among 18-64 years old in China. Improved nutritional status in China is needed by well-tailored strategies and policies.

 

Yongmin Xiong

Director of the Endemic Institute of Xi'an Jiaotong University, China

Title: The Study on GPX3 DNA Methylation for the Cartilage Damage Mechanism in Kashin-Beck Disease
Speaker
Biography:

YongMin Xiong holds the positions of director of the Endemic Institute of Xi'an Jiaotong University, deputy director of the Key Laboratory of Trace Element and Endemic Diseases of National Health and Family Planning Commission, China. Her qualifications include a Bachelor of Medicine, PhD of Medicine. She is currently the Committee member of Society of Endemic Diseases of Chinese Medical Association and chairman of Trace Elements Branch in Shaanxi Province Medical Association. She has a particular intrest in the environment and gene interactions in the molecular mechanism of endemic osteoarthritis diseases. She has published more than 30 articles in reputed journals.

 

Abstract:

Kashin-Beck disease (KBD) is an endemic and disabling osteoarthropathy. Epidemiological investigations have shown that selenium deficiency may contribute to the etiopathogenesis of KBD, however, the mechanisms of Se deficient in cartilage damage of KBD are still not clear. Glutathione peroxidases3 (GPX3), a member of selenoproteins, is found to paly a crucial role in protecting against oxidative damage in human body. The aim of the study was to investigate the effects of GPX3 DNA methylation on cartilage damage. GPX3 methylation were detected in whole blood of KBD patients and chondrocytes by methylation-specific polymerase chain reaction. The GPX3 mRNA were measured in whole blood of KBD patients and chondrocyte by qRT-PCR. The expression of PI3K/Akt/c-fos signaling molecules in whole blood of KBD patients and chondrocytes using Western blotting. The results showed that the frequency of GPX3 methylation significantly was increased and GPX3 mRNA was decreased in KBD patients than that of controls, and protein expression of Gβγ, PI3Kp110, pAkt and c-fos in KBD group were up-regulated. In the cell experiments, the rates of cell apoptosis and GPX3 methylation were increased, and GPX3 mRNA was decreased and PI3K/Akt/c-fos expression were up-regulated in injured group using tert-Butyl hydroperoxide. However, The rates of apoptosis and GPX3 methylation were decreased, GPX3 mRNA was increased and PI3K/Akt/c-fos signaling pathway were down-regulated in three Se pre-protection groups, suggesting that aberrant DNA methylation of GPX3 might be one of cartilage damage mechanisms in KBD (This research were supported by National Natural Science Foundation in China No.81573104, 81773372).

 

Speaker
Biography:

Ping Zhang, a graduate student in Jinan University, China, currently working in the Key Laboratory for Regenerative Medicine of the Ministry of Education and Division of Histology & Embryology, under the guidance of professor Xuesong Yang. Maintaining a high degree of enthusiasm and professional research attitude, she is been devoted into the program “Alcohol exposure induces chick craniofacial bone defects by negatively affecting cranial neural crest development” and has published the research results  on Toxicology Letters.

Abstract:

It is now known that excess alcohol consumption during pregnancy can cause fetal alcohol syndrome (FAS) in which several characteristic craniofacial abnormalities are often visible. However, the molecular mechanisms of how excess ethanol exposure affecting cranial neural crest cells (CNCCs), the progenitor cells of the cranial skeleton, is still not clear. In the study, we investigated the effects of ethanol exposure on CNCCs migration both in early chick embryo and in vitro explant culture. First of all, we demonstrated that ethanol treatment caused alizarin red-stained craniofacial developmental defects including parietal defect. Second, Immunofluorescent staining with neural crest special markers indicated that CNCCs generation was inhibited by ethanol exposure. And, double immunofluorescent stainings (Ap-2α/PHIS3, HNK1/BrdU and AP-2α/c-caspase3) revealed that ethanol exposure inhibited CNCCs proliferation and increased apoptosis. In addition, it inhibited NCCs production by repressing the expression level of key transcription factors which regulate neural crest development by altering expression of Epithelial-mesenchymal transition (EMT)-related adhesion molecules in the developing neural crests. In sum, we have provided experimental evidence that excess ethanol exposure during embryogenesis disrupts CNCCs survival, EMT and migration, which in turn causes defective cranial bone development.

 

Biography:

Xiaoxia Dai has completed her PhD in the year of 2006 from Xi’an Jiaotong University and postdoctoral studies from University of Iowa Carver College of Medicine, Department of Microbiology and Immunology. Now she is the researcher and teacher in School of Public Health, Xi′an Jiaotong University Health Science Center. She published more than 20 papers and has been serving as a member of the society of trace elements in shaanxi province.

 

Abstract:

Many studies suggest that adequate intake of Se is required to ensure optimal immune function and to prevent malignancy. Various components of the immune system fail to function correctly if there is dietary Se deficiency. In the present study, the effect of selenium supplements on the clearance of immune complexes of patients with KBD was evaluated. The effect of different selenium preparations on (1) erythrocyte selenium content, (2) activities of glutathione peroxidase, (3) erythrocyte immune adherence function, and (4) circulating immune complexes (CIC) content of patients with KBD was determined. The effect of sodium selenite and selenium yeast were also compared.

     In this randomized study, fifty Kashin-Beck disease patients from the endemic area, aged 13-16 years, were divided into two groups and were given either selenium yeast or sodium selenite orally to provide 200µg of selenium per day for 12 weeks. After supplementing with selenium for 12 weeks, erythrocyte selenium level, glutathione peroxidase activity, and E-C3bRR showed significantly high values, the difference in E-ICRR content was not significant. The effect of selenium in enhancing blood selenium levels and the clearance of immune complexes is greater when supplements provided as selenium yeast is used as compare to sodium selenite. This work was supported by the National Nature Scientific Foundation of China (Grant No. 81673117 and 81573140).

 

Biography:

Hafiz Arbab Sakandar is a graduate in School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People’s Republic of China.

Abstract:

The present study was undertaken to isolate and characterize gliadin hydrolyzing microbiota from locally fermented sourdoughs to evaluate their potential as a candidate for probiotics. Sixty autochthonous sourdough samples were collected from different areas. Eighteen bacterial and three yeast isolates were screened for the presence of gluten-degrading ability. These isolates were subcultured on different media for presumptive preselection by biochemical assays. Five bacterial isolates were LAB and two were bacillus among 18 bacterial isolates which were further characterized based on autoaggregation, ß-galactosidase activity, antibiotic susceptibility, inhibition against pathogens, bile salts tolerance, pH tolerance, surface hydrophobicity and cholesterol assimilation. In this study Lactobacillus plantrum ATCC 14917 was used as control strain to compare the probiotic potential of isolated strains. 16S and 28S rRNA sequencing revealed the presence of four Enterococcus faecalis strains along with Enterococcus mundtii, Bacillus cereus and Bacillus megaterium strains. Yeast strains were identified as Wicherhamomyces anomalus. Among all these strains E. mundtii QAUSD01 and W. anomalus QAUWA03 had the ability to tolerate low pH, bile salt properties and hydrophobicity compared to other gluten-degrading strains. These two strains exhibited the potential to be used as a probiotic for sourdough fermentation.

 

Speaker
Biography:

Souheir Alia, has her expertise in health education and promotion in both community setting as well as hospital setting, and works as a clinical dietitian in Rashid Hospital, DHA, in the city if Dubai, in UAE, and is in the process of finishing the requirement to finish her masters’ degree in food science, and her thesis being research based focusing on patients post bariatric surgery dietary protocol.

 

Abstract:

Obesity is considered to be a disease, which stands alone by itself, and it is accompanied by much co-morbidity, and that is why many means to treat obesity comes into account and one of the most prevalent ways in UAE is by bariatric surgery. Despite the vast research assessing nutrition knowledge of patients of several health conditions, none of the papers assessed the nutrition knowledge of patients’ post-bariatric surgeries, although this category of patients is very susceptible to malnutrition post-surgery. My aim in this study was to assess the general nutrition knowledge and the knowledge specific to the dietary protocol post-surgery, the medical and nutritional complications, and their awareness and understanding of dumping syndrome specifically, the clarity of information conveyed by the dietitians and its effect on their levels of compliance of the post-bariatric surgery dietary protocol and the follow up appointments with the dietitians, and finally a small part gave a glimpse of their quality of life post-surgery.

         The methods used to analyze the questionnaire were with the help of SPSS version 23.0. Descriptive statistics such as frequencies, proportions, means, and standard deviations were used. Statistical tests such as Chi-Square test of independence, and Pearson’s correlation were used to test correlation. To test significant differences between values of quantitative variable were used using the statistical test ANOVA or its equivalent non-parametric test named Kruskal-Wallis. Normality was tested using the Shapiro-Wilk test, while Levene’s test was used to test the equality of variance.

           The results of this study showed a fairly good general nutrition knowledge of both groups, the questions assessing nutrition knowledge of the dietary protocol post-surgery were added up and given a score out of 14, in which it showed that only 19.4% of participants had very good knowledge, 66.2% had average knowledge, and 14.4% had poor knowledge. In addition, most patients didn’t know what dumping syndrome is, and of those who knew what it is 66.6% of them knew the food that promote its occurrence, and almost half of the participants who answered yes knew the symptoms of it. On the other hand, 79.5% of the patients followed up with a dietitian, and only 30.1% showed compliance to the dietitian’s instructions, which was strongly related to patients finding the information conveyed vague and unclear as 71.2% considered it as aforementioned. However, the most experienced symptom post-bariatric surgeries were nausea, followed by dizziness, dehydration, and finally vomiting. As for the overall quality of life of participants the highest percentage 45.8% was given to participants who never felt agitated, fatigued and/or regretted their decision of getting operated, and as much as 83.1% found their daily activities to be more enjoyable. In conclusion, patients who undergo bariatric surgeries are a great area of improvement now that we can spot some gaps in the health care provided. 

Speaker
Biography:

Mona Ahmed J. Alzahrani is a PhD student at the School of Chemical Sciences – Food Science. Mona completed her master’s degree in Engineering, specialized in chemical and materials engineering from the University of Auckland (2011). Mona also studied a PGDip in Science – Food Science at the University of Auckland. In 2007, Mona obtained her Bachelor’s degree from Umm Al-Qura University in Saudi Arabia specialized in Nutrition and Food sciences. Professionally, Mona has managed a number of municipal health-related projects in Saudi Arabia. Mona’s research interests lies in novel food processing technologies, mainly as an aid for extraction process of bioactive compounds from microalgae as a sustainable approach compared to conventional sources.

Abstract:

Nitzschia laevis is used to extract highly pure eicosapentaenoic acid (EPA) for commercial use in food and pharmaceutical applications, and the biowaste is used for animal feed. This research focuses on the aqueous production of bioactive proteins and hydrolysates from the by-product of EPA production from N. laevis.  A comparison of antioxidant and antihypertensive activities was established between the extracts of N. laevis and other well-known microalgae that are used as food ingredients/supplements.  In this study: 1) Proteins were extracted from N. laevis,  2) Hydrolysates were produced from proteins extracts using Alcalase®CLEA™, Flavourzyme® and Trypsin, 3) Proteins and hydrolysates were purified and partially characterized, 4) in vitro bioactivities were screened using chemical and enzymatic assays. The hydrolysis process enhanced the antioxidant and antihypertension activities in general, especially those obtained using Alcalase®CLEA™. Nitzschia showed the highest total phenolic content and reducing capacity (2.4±0.02mg GAE/100g) after 90 minutes of hydrolysis with Alcalase®CLEA™. Moreover, hydrolysates at 120 minutes showed the highest ABTS scavenging activity (66.77±0.003%), but DPPH was low (29.599±0.024%). A correlation study was established between DPPH and ABTS scavenging activity with total phenolic contents, trypsin hydrolysates showed the highest positive correlation. The antihypertensive activity was significantly enhanced after hydrolysis; Alcalase®CLEA™ hydrolysates of N. laevis showed higher ACE inhibition activity after 30 minutes (64.81% ACE inhibition) and Flavourzyme® hydrolysates also showed high activity after 60 minutes (60.81% ACE inhibition) compared to controls. The interactions between proteins and hydrolysates with other residues are likely to contribute to the measured antioxidant and antihypertension activities.

 

Biography:

Dr.Nisreen Rajeh has completed her PhD at the age of 40 years from SurreyUniversity (UK) and postdoctoral studies from KAU University School of Medicine. she has published more than 10 papers in reputed journals.

Abstract:

Objectives:

To explore renal toxicity caused by sub-acute exposure of acrylamide, which is known food toxicant , and to study the protective effect of 5-Aminosalicylic acid (5-ASA) and Vitamin E (vit-E)on Acrylamide (ACR) induced renal toxicity.

Methods:

This study was conducted at King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, between August and November 2015. A total of 49 adult Wistar rats (250 } 20g) aged 60 days were kept in a controlled environment and used in the present study. The rats were divided into 7 groups (control, ACR alone, ACR+5-ASA, ACR+vit-E, ACR+ASA+vit-E, vit-E alone, and ASA alone). After 5 days of ACR oral gavage treatment, the rats were observed for 24 hours then killed. Histopathology for the kidney and lactate dehydrogenase assay were carried out.

Results:

Acrylamide produced significant pathological changes in the kidney with acute tubular necrosis in the distal tubules that could be reversed by concomitant injection of rat with 5-ASA. Together with vitamin E, 5-ASA, showed maximum renal protection. No statistically significant difference was observed in either body weights or lactate dehydrogenase activity of ACR treated rats.

Conclusion:

Acrylamide exposure leads to adverse clinical pathologies of renal tubules, which were reversed by a concomitant treatment with 5-ASA and vitamin-E .

 

Biography:

Gülşen Delikanlı is a research assistant in Department of Nutrition and Dietetics, Trabzon, Turkey.

Abstract:

Statement of the Problem: It’s been found that many factors such as education and socioeconomic level are effective on breastfeeding and infant feeding practices in studies conducted in developed and developing countries. Due to untrained and unconscious eating behaviors of the mothers, the breastfeeding activity is not ensured sufficiently. The level of development of the countries is determined by the health level of the children in that country and compared with the child mortality rate. The purpose of this study is to determine the knowledge levels and behaviors for breastfeeding and complementary nutrition of mothers 0-24 month postpartum. Method:The age, occupation and educational level of 250 women, the duration and frequency of breastfeeding of the infants were questioned.The level of knowledge and applications of maternal breastfeeding and infant feeding have been determined. Results: Mothers with low levels of education and socioeconomic status have worse knowledge of infant feeding. There was a statistically significant difference with breastfeeding duration and thinking to continue breastfeeding with the increase in the educational level. There was no statistical significance in the level of knowledge and practice according to education level. Conclusion: The knowledge level of women about breastfeeding and infant feeding varies according to the level of education and the place they live. Increasing the education level of women, developing courses and skills is important for healthy infants and society. All employees involved in basic health services should be educated about the importance of breast milk and breastfeeding.

Nilva Kazue Sakomura

Universidade Estadual Paulista “Júlio de Mesquita Filho”, Brazil

Title: Optimal tryptophan intake for laying hens
Speaker
Biography:

Nilva Kazue Sakomura has completed her PhD at Universidade Federal de Viçosa and postdoctoral studies at University of Arkansas (USA) and University of KwaZulu-Natal (RSA). She acts as professor at the Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCAV/UNESP and coordinates the Laboratory of Avian Sciences at same university. She participated in the elaboration of the last edition of Brazilian Tables for Poultry and Swine. Her research area is mainly foccused on modeling amino acid requirements of broilers, laying hens and broiler breeders.           

Abstract:

Modern poultry industry has been gradually decreasing crude protein content of poultry diets and meeting poultry amino acid requirement more precisely, in order to save production costs and reduce the negative impact of livestock on environment. In this context, determining tryptophan (Trp) requirements of laying hens could allow further reductions on dietary CP supply. A total of sixty 29-week-old Hysex White laying hens were individually allocated into 6 dietary treatments with 10 replicates. The 6 Trp levels studied in this trial (0.81; 1.06; 1.50; 1.86; 2.05 and 2.30 g/kg) were obtained using the dilution technique. The trial lasted 10 weeks, but only those from the final 4 weeks were used in subsequent analysis. Optimal Trp intake was estimated using polynomial, broken-line regression model and the association between both models. Graded Trp intake elicited a quadratic response on egg production (EP) and egg output (EO), which were maximized at 196 and 208 mg Trp/bird/day, respectively, whereas feed conversion ratio per egg output (FCR) were optimized at 185 mg Trp/bird/day. According to broken-line regression model, the breakpoint estimated for maximum EP and EO; and optimal FCR occurred with a daily Trp intake of 117, 134 and 50 mg/bird. The association between polynomial and broken-line regression models produced the optimal Trp intake of 161, 173 and 66 mg/bird/day for EP, EO and FCR. Based on EO responses estimated by associating both regression models, the optimal Trp intake for laying hens is 173 mg/bird/day.

 

Nilva Kazue Sakomura

Universidade Estadual Paulista “Júlio de Mesquita Filho”, Brazil

Title: Ideal in-feed amino acid ratio for laying hens
Speaker
Biography:

Nilva Kazue Sakomura has completed her PhD at Universidade Federal de Viçosa and postdoctoral studies at University of Arkansas (USA) and University of KwaZulu-Natal (RSA). She acts as professor at the Universidade Estadual Paulista “Júlio de Mesquita Filho”, FCAV/UNESP and coordinates the Laboratory of Avian Sciences at same university. She participated in the elaboration of the last edition of Brazilian Tables for Poultry and Swine. Her research area is mainly foccused on modeling amino acid requirements of broilers, laying hens and broiler breeders.           

Abstract:

The research was conducted to re-evaluate the assumptions of ideal ratios (IAAR) of the essential amino acids for Hy-line W-36 laying hens, using the amino acid deletion and nitrogen balance methods. The nitrogen balance trial was performed from 28 to 32 weeks of age, 12 treatments with 8 replicates and 1 bird per cage were used. A balanced diet (BD) was formulated to meet the IAAR and the requirement of other nutrients. The limiting diets were formulated diluting BD with corn starch and refilled with synthetic amino acids (AA) and other feed ingredients, except for the AA studied. The trial lasted 25 d, being the first 5 d for adaptation and the other 20 d for total collection of excreta and eggs. Diets, excreta and eggs samples were analyzed for nitrogen content to quantify nitrogen intake and excretion. From these data, nitrogen retention (NR) was calculated. The estimated requirement of each AA (AAR) was calculated as AAR= 100 x (1-NRi/NRcontrol) where NRi is the NR associated with each limiting diet calculated and NRcontrol is the NR associated with the balanced control diet. The estimated optimum ratio between each AA and Lys [(AA/Lys) * 100] and the optimum level of each AA (values in parentheses) were: Lys = 100 (0.673), Met+Cys = 82 (0.552), Thr = 69 (0.464), Trp = 21 (0.141), Arg = 104 (0.700), Val = 91 (0.612), Ile = 78 (0.525), Leu = 121 (0.814), Phe+Tyr = 119 (0.801), Gly+Ser = 77 (0.518) and His = 29 (0.195).

 

Speaker
Biography:

Nabil Bosco currently works at the Nestlé Research Center, Nestlé S.A.. Nabil does research in Human Biology, Cell Biology and Immunology. 

Abstract:

BACKGROUND

Elderly adults over 65 years of age are recommended to receive seasonal influenza vaccination as they are at a higher risk of infection than the younger community. The elderly population is often stratified according to frailty status where frail individuals are more susceptible to adverse health outcomes than their non-frail counterparts, however, it is not known whether protective immunity induced by influenza vaccination is impaired in the frail elderly.

STUDY DESIGN

205 elderly subjects of Chinese ethnicity in Singapore (mean age 73.3 ± 5.3 years, 128 females and 77 males) were administered the recommended trivalent inactivated 2013-14 seasonal influenza vaccine (VaxigripÔ) containing A/H1N1, A/H3N2 and B strains. The elderly subjects were stratified into three groups according to Fried’s frailty criteria (59 frail, 85 pre-frail, 61 robust) and were also ranked by Rockwood’s frailty index (RFI) or nutritional status. Statistical associations were evaluated between frailty status, pre- and post-vaccination antibody titers in sera, modulation of leukocyte populations, cytokine responses and gene expression profiles of peripheral blood mononuclear cells.

RESULTS

Post-vaccination, 93.2% of the elderly were seroprotected against A/H1N1, 97.1% against A/H3N2 and 99.5% against the B strain. Seroprotection rates were similar in all three frailty groups (Anova, A/H1N1, p = 0.605; A/H3N2, p = 0.585, B, p = 0.585) and uncorrelated with RFI (Spearman, r = 0.023, p = 0.738). No statistically significant differences were observed in vaccine-induced modulation of leukocyte populations, cytokine responses and gene expression profiles of peripheral blood mononuclear cells.

CONCLUSION

The majority of the elderly subjects were seroprotected against seasonal influenza upon vaccination, and importantly, influenza vaccination-induced humoral immune responses and seroprotection were similar across the frailty strata, indicating that frail individuals may also benefit from influenza vaccination.

 

Speaker
Biography:

Joao Batista Kochenborger Fernandes has completed his PhD at the age of 25 years from “Universidade Estadual Paulista Júlio de Mesquita Filho”, UNESP, Brazil and postdoctoral studies from “University of Arkansas, USA and University of Kwazulu-Natal”, UKZN, South Africa. Since 1990, he has been a Specialized Fish Technician at the Aquaculture Center of UNESP, Jaboticabal-SP, Brazil, developing activities with rural producers through consultancies, projects and technical assistance.

 

Abstract:

The aquaculture look for more and more an efficient production, less environmental impact and increased profitability. Knowing that the correct feeding and balanced diet promotes maximization of growth and health, reduce the production costs and water pollution, the present study aimed to estimate the essential amino acids (EAA) ratio for juveniles of pacu (Piaractus mesopotamicus) using the deletion method. For this, 635 pacus (6.23g) were distributed in thirty three tanks connected to a closed water recirculation system equipped with filter, water heater (27.8ºC) and aeration. The experiment lasted 113 days and consisted of 11 experimental diets with three replications each, distributed in a completely randomized design. The fishes were fed twice a day until apparent satiation. The semi-purified and extruded diets were formulated by the deletion technique, where the control diet presented all the EAA in a balanced way, while the experimental diets presented the deletion of 45% of the respective tested EAA. In this way it was possible to obtain 11 isonutrient diets varying only the tested EAA. The determination of the essential amino acids ratio was performed by the ratio between the nitrogen retention in fish body and the amount of the EAA deleted in the tested diet. Thus, assuming that each EAA is equally limiting, our results show the ideal dietary EAA profile relative to lysine was estimate to be: lysine 1.000, arginine 0.593, threonine 0.336, valine 0.282, leucine 0.396, isoleucine 0.220, phenylalanine 0.226, methionine 0.126, tryptophan 0.063.

 

Pedro M. Rodrigues

Universidade do Algarve, Portugal.

Title: Allergenicity modulation of farmed fish
Speaker
Biography:

Pedro M. Rodrigues has a PhD in Chemistry from the Universidade Nova de Lisboa and is presently a Professor at University of Algarve, Portugal since 2000 and a member of the Center of Marine Science of the Algarve. He has been working in the use of proteomics in Aquaculture to access fish quality, welfare and allergies for the past 10 years and he has been involved in several research projects, two COST actions and published 34 papers in reputed journals, 3 book chapters and has also been serving as an editorial board member of repute.

 

Abstract:

Food allergies are a significant public health concern throughout the world. Fish is a food product increasingly consumed worldwide due to its high nutritional value and healthy meat. Unfortunately, like many food sources, fish can cause adverse immune-mediate reactions in some individuals. This allergic reaction to food, affects 1-3% of the population and about 4-6% of children. The main fish allergen is β-parvalbumin; a small and highly stable muscle protein. Fish parvalbumins are highly conserved proteins, which are binding bivalent ions, calcium or magnesium. In fish-allergic patients, specific IgE cross-react mostly with parvalbumins from different fish, especially when recognizing highly identical protein regions involved in the ion binding. Other fish allergens have been identified as well - such as enolases, aldolases or fish gelatin - but their importance has been only shown for a limited number of fish species. Currently, there is no cure available for fish allergies with the clinical management of fish allergy solely relying on a strict avoidance diet. Modulation of fish allergenicity towards the production of a low allergen farmed fish was firstly attempted by our group. This has been done with specifically designed fish diets, enriched in components and small changes introduced in the fish farming process that target the expression or inactivation of the main fish allergen; parvalbumin. Proteomics is the chosen technique to access fish allergens characterization and expression in muscle while IgE assays to confirm the lower allergenic potential of this fish are conducted in patients with history of fish allergies.

 

Speaker
Biography:

Mr. Selekane Ananias Motadi is a junior lecturer of Nutrition at the University of Venda. He received his junior degree BSc in Nutrition at the University of Venda. In 2010, He registered for Master Degree in Public Nutrition at the same university. He is a registered Nutritionist with Health Professional Council of South Africa. He was offered tenure in the Department of Nutrition. In addition to teaching, Mr. Motadi is a regular contributor to the micronutrient malnutrition particularly zinc and a Mamelodi sundowns United fan. He has registered for postgraduate diploma in health professional education at the University of Cape Town which he envisages completing in 2015. He has collaborated on manuscripts with Prof XG Mbhenyane, Dr. RL Mamabolo, Ms. HV Mbhatsani and Mr. NS Mabapa entitled “Prevalence of zinc deficiency among children aged 3-5 years in Vhembe district, Limpopo province, South Africa”. He currently resides in Limpopo Province, South Africa with his brother.

 

Abstract:

Objective: To describe the prevalence of overweight and obesity among adults aged 18-45 years residing in and around Giyani town.

Methods: This study included 100 participants recruited from a location in Greater Giyani Municipality of Mopani district, Limpopo Province, South Africa. This Municipality was purposively selected and convenience sampling was used to choose study participants. Body weight and height were measured using standard techniques. Waist circumference, systolic, diastolic, glucose, cholesterol and body fat levels were also assessed. 

Results: The prevalence of overweight and obesity was 18.1% and 20.5%, respectively. Obesity was more prevalent in females (19%) than males (1.5%). The risks found in study participants included hypertension, diabetes, high total cholesterol, alcohol consumption and cigarette smoking. There was a significant difference in systolic blood pressure (p≤0.03) between males and females.  Systolic blood pressure of about 22.9% of male participants compared to females (20%) was in the hypertensive range.  However, only 9.2% of females had prehypertension of between 85-89 mmHg. Furthermore, 17.1% of males had hypertension values ≥90 mmHg as compared to 9.2% of their female counterparts.

Conclusion: Overweight and obesity were common in adults residing in urban areas. The results observed here call for intervention to combat the rising problem of overweight and obesity among adults in the form of nutrition education to ensure food diversification and reduction of food containing fats and hidden fats.

Keywords: overweight, obesity, body mass index, body fat, waist circumference, total cholesterol.

Speaker
Biography:

Su Bin Park has been completing her master’s degree from Gyeongsang National University, division of Agriculture and Life science. She has published two domestic papers associated with functional foods

 

Abstract:

To estimate physiological effect of ethylacetate fraction of Aruncus dioicus var. kamtschaticus (EFAD) on cognitive dysfunction, in vivo and ex vivo test were conducted in neurodegenerative animal model. The animal model is established as injecting trimethyltin (TMT) (7.1 𝜇g/kg of body weight, intraperitoneal injection) in Institute of Cancer Research (ICR) mice. EFAD showed the excellent ameliorating effects on reduced spatial memory ability, short- and long- term memory deficits in the mice through in vivo tests such as the Y-maze, passive avoidance and Morris water maze test. After the behavioral tests, to ascertain the protective effects of EFAD on the cholinergic and antioxidant system, a series of biochemical indicators were measured in brain tissue of mice. Acetylcholine (ACh) as a neurotransmitter and acetylcholinesterase (AChE) as an enzyme that catalyzes the breakdown ACh were measured. EFAD has been proved to have the significant effects on cholinergic system by increasing ACh levles and decreasing AChE activity. In addition, EFAD improve the antioxidant system by diminishing malondialdehyde (MDA), an indicator of lipid peroxide, and elevating antioxidant such as contents of reduced glutathione (GSH) and superoxide dismutase (SOD).

 

Speaker
Biography:

Jin Yong Kang is Ph.D student at Gyeongsang National University (Jinju 52828, Republic of Korea). He is mainly studing on functional foods. He has published 2 papers in SCI(E) journals and participated as a co-author in more than 5  paper.

Abstract:

This study was performed to confirm the influence of chlorogenic acid (CGA) and 3,5-dicaffeyolquinic acid (3,5-diCQA) intake on disorders caused by high-fat diets. They have been shown to improve impared glucose tolerance and inhibit weight gain caused by high fat diet although CGA and 3,5-diCQA differ in their activity. In biopsy, it is confirmed that CGA inhibits visceral fat and liver fat accumulation, and 3,5-diCQA also inhibited visceral fat accumulation, but a 3,5-diCQA increased liver fat accumulation. Both CGA and 3,5-diCQA inhibit accumulation of visceral fat by stimulating AMPK phosphorylation in epididymal white adipose tissue. CGA also increased AMPK phosphorylation in the liver tissue, but 3,5-diCQA reduced AMPK phosphorylation. This result in liver tissue is presumed to be related to Akt phosphorylation. AKT phosphorylation was significantly increased in liver of the 3,5-diCQA group. Increased AKT phosphorylation improved glucose tolerance, but it inhibted AMPK phosphorylation and thereby increase fat accumulation in liver. In addtion, as a result of confirming the degree of liver damage, the liver fat accumulation induces oxidative stress, but 3,5-diCQA reduced oxidative damage through its antioxidant activity. To sum up, a CGA is effective in improving the problems caused by high-fat diets while maintaining balance. A 3,5-diCQA also has shown a positive effect on problems caused by high-fat diets, but it increased liver fat accumulation and thereby cause negative consequences.

 

Speaker
Biography:

Yongmin Kim is PhD student in Bioinformatic at Kangwon National University in Korea and works at National Instititue of Animal Science as Junior Researcher. I’m interested in animal breeding and meat quality of swine.

 

Abstract:

This study was conducted to determine the effects of breed and sex on meat quality and sensory properties of the loin in three-way crossbred pigs: Landrace × Yorkshire × Duroc (LYD) and Landrace × Yorkshire × Woori black pig, as Korean native pig (LYW). Carcass traits did not differ by breed. Carcass weight and backfat thickness were higher in castrates than in gilts (p < 0.01). Fat content, cooking loss, and water-holding capacity were higher in LYW than LYD (p < 0.05). Redness and yellowness of the meat were higher in LYW than in LYD (p < 0.01). Further, LYW had lower pH and shear force than LYD (p < 0.001). Two sensory properties, color and flavor, were better in LYW than in LYD, and in gilts than in castrates (p < 0.05). However, other sensory traits did not differ by breed or sex. Capric acid (C10:0) was higher in LYD than LYW (p < 0.001). However, stearic acid (C18:0) and saturated fatty acid (SFA) contents were higher in LYW than LYD (p < 0.05). Eicosenoic acid (C20:2) and the n6/n3 ratio were higher in gilts than in castrates, whereas SFA content was higher in castrates than in gilts. These results suggest that certain physicochemical qualities of meat and sensory properties are improved in LYW as compared to LYD. This study provided basic data on meat quality of crossbred pigs with Woori black pig as a terminal sire.

 

Speaker
Biography:

Jan Petros J. Esparagoza, a 20 year old student from the University of the Philippines, Manila, is currently undergoing his medical degree.

Abstract:

Schools are essential environments for growth and development of both students and faculties. However, there are gaps in the literature regarding food environment in the Philippines particularly around schools. As such, the study characterized the food environment around a university in Manila in terms of food availability and food accessibility.

            A descriptive research design was utilized and total enumeration of food stores within three blocks from the campus was done. Food availability was assessed through direct observation using EPI Info v. 7 and was analyzed through descriptive statistics. Food accessibility was assessed using a mobile GPS application and was visualized through Arc GIS v. 10 through kernel density mapping and hotspot mapping. Buffers were also set at 100 meters and 200 meters from the centroids assigned.

            High-sugar/energy-dense beverages, empty calories and canned meat products were found to be sold immediately outside the campus, and were more prevalent compared to healthier alternatives. Only a few food stores sold vegetables and fruits, with a few found immediate outside. In addition, stores that do sell fruits/vegetables were found to have only a few varieties, with only one store selling more than 10 kinds of fresh produce within 200 meters from the centroids in the campus.

            Findings showed that the food environment around the university is potentially obesogenic and is less capable of providing the essential nutrients needed by an individual. Thus, a healthy food environment should be promoted within and around the university to encourage its constituents to avail of healthier food products.

 

Speaker
Biography:

Cherry Ann Garcia is a nurse and an educator. She is passionate in conducting free public health teachings in rural communities in their province. Her expertise is on medical-surgical nursing and psychotherapy and counseling. She is a pastor’s wife.

 

Abstract:

INTRODUCTION:

Atopic dermatitis (AD), also known as eczema, is the most common chronic relapsing skin disease in children, affecting approximately 10% to 30% of children worldwide. For this reason, the research community investigated possible innovative prevention and treatment strategies for AD. One of these strategies was the manipulation of the intestinal flora through probiotics.

METHOD:

This study is a cross-sectional, analytic study on 680 mothers and 680 children recruited in selected urban communities in Laguna, Philippines.

RESULTS:

Most mothers (92%, n=625) had highly positive attitude to probiotics. Composite scores were computed and statistical data revealed that attitude is significantly correlated with behavioral control (r=0.2087, p-value <0.001) and intention (r=0.2934, p-value <0.001). Furthermore, it was found out that both intention (r=0.3703, p-value <0.001) and perceived control (r=0.2355, p-value <0.001) were significantly correlated to total amount of intake of probiotic food. Female children (42.43%) have higher intake than males (30.61%). High intake was also noted among children of mothers with educational attainment of Vocational (45.45%) and College and higher (41.09%); among low- (38.92%) and middle-income families (44.06%); and among those without family history of AD (38.55%). As for the frequency of consumption of probiotic food, 3 out of 10 of the respondents reported daily intake of at least one bottle of the probiotic foods enumerated. Among 680 respondents, 18.09% (n=123) were diagnosed as having atopic dermatitis, while 81.91% (n=557) were without AD. This study showed that the odds of having atopic dermatitis was 2.4 times higher among those with low intake status and 4.3 times higher among those with none intake status compared to children with high intake status.

CONCLUSION:

Intake of probiotics are positively correlated with maternal education, income, highly positive attitude, high perceived control, and intention of mothers. Consumption of probiotics is a protective factor against atopic dermatitis.

 

Speaker
Biography:

Edzil C. Besonia has completed her MS Food Science and Technology in Visayas State University, Philippines.

Abstract:

Information about food composition is necessary for the assessment of diet quality, development and application of food-based dietary guidelines, providing a useful tool for the field of public health nutrition. The study was conducted to determine the effects of varying levels of frying time, cooking time and spin-drying time on the physico-chemical properties of vacuum fried cassava bites. A 33 fractional factorial experiment following Central Composite Design with different levels of frying time (30, 45, 60 minutes), cooking time (10, 15, 20 minutes) and spin-drying time (2, 4, 6 minutes) were used. Data for all experimental combinations were analyzed employing response surface regression analysis. The physico-chemical, nutritional and proximate, water activity, microbial and storage properties of the optimum process of vacuum fried cassava bites with 50 minutes frying time, 20 minutes cooking time and 6 minutes spin-drying time were evaluated.

            Results on the response surface regression on the physico-chemical properties of the vacuum fried cassava bites revealed significant effects on the product except for the percent recovery and b* color unit. Optimized product has high calorie content along with the other macronutrient like fat, carbohydrates, sodium and crude protein. Storage stability of the product in the weekly monitoring of moisture content, water activity, color determination and sensorial descriptions showed significant difference over the observation period. Evaluation for total plate count and presence of yeast and molds showed a negative result.

 

Speaker
Biography:

Ronalyn V. Amper has completed her MS in Visayas State University, Philippines.

Abstract:

The combination action of α-amylase, xylanase and composite flour was analysed to determine their effects on the sensory quality of the bread through optimization using Response Surface Methodology (Central Composite Design). Fifteen bread formulations containing different concentrations of α-amylase (0, 0.0005 and 0.003% w/w), xylanase (0, 0.001 and 0.003% w/w) and composite flour (15, 30, 45%w/w) were used in the analysis. The study revealed that the addition of α-amylase had significant influence on the response of crust color, crumb structure, flavor and general acceptability of the bread. The presence of xylanase causes a change on the response of crust color, crumb texture and the general acceptability only, however, its crossproduct interaction with composite flour level affect the response of crust color, crumb texture and structure, flavor so as the overall acceptability of the product. The synergistic effect of α-amylase and xylanase displayed an influence positively on the overall acceptability of the bread. The optimum formulation set at acceptability rating of >7.20 on the 9-point Hedonic scale was determined at 38% composite flour with 0.002% α-amylase and 0.0012% xylanase considering a cost of P41.14 per 421.27g of loaf. The observed acceptability mean for all sensory attributes of the formulation was not significantly different with the predicted acceptability mean value of the model according to verification test. This formulation was not significantly different with the commercial bread available in the local market of Baybay, Leyte based on the consumer acceptance results with children and adults as potential target market.

 

Biography:

Anna Monica B. Dumalaog is a graduate in Department of Food Science and Nutrition, University of the Philippines Diliman, Quezon City Philippines.

Abstract:

Student-athletes from weight-class sports are exposed to high pressure conditions due to heavy academic load and frequent weigh-ins. Collegiate Varsity Members drastically change food intake before weigh-ins to meet specific weight classes. Such conditions lead to the development of eating disorders and binge diets, and as a result, excess and severe depletion of nutrient stores may pose adverse health effects later on, alongside decreased athletic performance. Thus, proper nutrition management is needed. The study investigated the differences in energy balance of Collegiate Athletes before, during, and after training days. Study participants from the University of the Philippines Judo Team (n=20) completed an adapted Nutrition Knowledge, Attitudes, and Practices Questionnaires, 3-day non-consecutive 24-hour estimated food records, and 24-hour physical activity diaries before, during, and after the training days. Through the one tailed t-test (p=0.05), a significant difference was recorded between the mean caloric intake of participants from training to post training days (t=-1.967). Meanwhile, the caloric expenditure of participants were significantly higher during training days, compared to no training (t=5.03) and post training (t=6.42) days. This difference in caloric intake and expenditure may lead to athletes developing lifestyle diseases such as obesity if they decide not to pursue Judo in later life. Pearson correlation coefficient test between nutrition knowledge and attitude scores to energy balance of participants showed only minimal correlation between the variables. No correlation was found between nutrition knowledge scores and energy balance  (R=0.00). On the other hand, a weak positive correlation was found between the score for sports and nutrition attitudes and energy balance. (R=0.33). Present data suggest that a more positive energy balance, or less negative energy balance can be weakly associated with increased preference towards the power and performance model of sports participation.

 

Speaker
Biography:

Garner  has recently completed his Masters of Science in Fisheries-Fish Processing Technology  at the age of 24 years from University of the Philippines Visayas.      

Abstract:

Skin gelatin of Oneknife unicorn fish (Naso thynnoides) was hydrolysed using a crude protease from Bacillus sp. under optimum hydrolysis conditions. Resulting hydrolysates was subjected to centrifugal ultrafiltration to produce fractions of ≤10KDa molecular weight. Antioxidant, antihypertensive and functional properties of the hydrolysate fraction were determined. Results showed that DPPH (a,a-diphenyl-b-picrylhydrazyl) scavenging activity (63%) and ferric reducing antioxidant power (25.90 Trolox Equivalent (mM/mg)) increased as protein concentration increased. Angiotensin converting enzyme-I (ACE-I) inhibitory activity is directly proportional to the protein concentration with highest value obtained at 33.97% and IC50 determined to be 10.17 µg /ml. Gel electrophoresis (SDS-PAGE) revealed that the gelatin hydrolysates contain mostly peptides with molecular weight ranging between 5 KDa and 30 KDa. Amino acid profile of the hydrolysates showed that it is rich in residues of glycine (Gly) (40.70%) and glutamic acid+glutamine (Glx) (25.40%). The hydrolysates was soluble over wide pH range (79.38 - 97.12%). Foaming properties increased while emulsion properties decreased as the gelatin hydrolysate concentration was increased. Results of the present study revealed that Oneknife unicornfish gelatin hydrolysates could be a potential food ingredient with antioxidant and antihypertensive properties.

 

Biography:

Precious Dee L. Herbalega is a graduate student in University of the Philippines Visayas, Miagao, Iloilo 5023 Philippines.

 

Abstract:

Lactic acid bacteria (LAB) from Philippine traditional fermented rice-shrimp mixture locally known as Balao-balao was isolated and screened. From a total of 20 selected acidproducing isolates, four isolates (LM8, LM12, LM17, and LM20) showed promising inhibitory activity against pathogens namely S. aureus, E.coli, B. subtilis and V. harveyi. Among the four isolates, LM12 showed the highest inhibitory activity. Optimum incubation period to achieve the highest activity was observed at 48 h. Isolate LM12 was identified as Pediococcus pentosaceus (Genbank Accession No. KY689941) by phenotypical tests and 16s rDNA analysis. Complete inactivation on the activity of the cell free supernatant was observed after treatment with trypsin and proteinase-K. The inhibitory activity was not affected by the addition of catalase and hydrogen peroxide, thus, the antimicrobial substance was proteinaceous in nature. Its molecular weight was approximately 5 kDa by tris-tricine SDS-PAGE. The bacteriocin of P. pentosaceus LM12 was sensitive to proteases but insensitive to lipase and amylase. It was also pH (pH 2.011.0) and temperature stable (0ᵒC-121ᵒC). After testing P. pentosaceus LM12 bacteriocin, results showed that it was active against both Gram-positive and Gram-negative bacteria. This is the first report of a bacteriocin-producing P. pentosaceus isolated from a fermented fish product with positive antagonism against Gram-positive and Gram- negative bacteria. 

 

Speaker
Biography:

Nahla Mohamed has completed her MS in Food Chemistry at the American University in Cairo, School of Sciences and Engineering, Chemistry Department. She is a Research Assistance at Chemistry Department, American University in Cairo.

 

Abstract:

Consumer demand for ready-to-eat ‘fresh’ and safe food products with less synthetic preservatives together with well documented food-borne microbial outbreaks drive both research and food industry toward new innovative methods for microbial growth inhibition while keeping food freshness, quality, and safety. Incorporation of natural bioactive agents in the packaging material to increase the shelf life of meat products is a promising technology. Grapes are of special interest because of their high content of phenolic compounds that showed antimicrobial and antioxidant effects. The aim of the present work was to investigate grape seed (GSE) and skin (GSKE) extracts’ antibacterial activity and developing bioactive LDPE/PET films that could be used as food packaging for poultry and meat products. Commercial corona treated LDPE and PET were coated with either grape seed or grape skin extract. Agar plate diffusion method was used for the investigation of the microbial properties of both extracts’ coated films against E.coli as a Gram-negative bacterium and Staphylococcus aureus as a Gram-negative one. LDPE and PET films coated with GSE showed inhibition zones of E.coli growth in the range of 16-25mm, while Staph.aureus growth inhibition zones were in the range of 15-20mm. For LDPE corona films coated with grape seed extract, the minimum inhibitory concentration (MIC) was 0.002g/1cm2 for E.coli and 0.003g/1cm2 for Staph.aureus. While for corona treated PET films/GSE, the MIC for both E.coli and Staph.aureus was 0.002g/1cm2. Corona treated LDPE and PET coated with GSKE showed inhibition zone range of 13-16.3mm with E.coli and 12-20mm with Staph.aureus.  For LDPE corona films/GSKE, the minimum inhibitory concentration (MIC) was 0.0009g/1cm2for E.coli and 0.003g/1cm2for Staph.aureus. While for corona treated PET films/GSKE, the MIC was 0.002g/1cm2 for E.coli and 0.003g/1cm2 for Staph. aureus. The Total Phenolic Content of both GSE and GSKE was determined using the Folin- Ciocalteu methodology to be 315.32g (GAE)/kg, and 265.326g (GAE)/kg for GSE and GSKE respectively.  The coated films; LDPE/GSE or GSKE, were used to wrap fresh chicken fillets, TVC, Pesudmonads, Brochothrix thermosphacta, Lactic acid bacteria and Enterobacteriaceae counts were determined during the storage period; 10 days for test samples and 8 days for controls. Microbiological analysis for tested samples was done on day 0, 2,4, 6, 8, and10, while for control till day 8. There was a reduction in the populations of the examined bacteria in the range of 0.2-1.4 log cfu/g in case of GSE, while with GSKE the reduction of bacterial populations range was 0.3-1.95 log cfu/g.